Guinea Pig ECG Changes under the Effect of New Drug Candidate
TP28b

k 2021

Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b

BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ a Marie NOVÁKOVÁ

Základní údaje

Originální název

Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b

Název česky

Anestezované morče jako model pro testování nových léčiv

Autoři

BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ a Marie NOVÁKOVÁ

Vydání

48. ročník konference "Computing in Cardiology" 2021

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

morče, celotělový model, beta-blokátor, arytmogenní potenciál

Klíčová slova anglicky

guinea pig, whole body model, beta-blocker, arrhythmogenic potential

Příznaky

Mezinárodní význam

Změněno: 18. 1. 2022 08:18, Mgr. Tereza Miškechová

Anotace

V originále

Introduction: Anesthetised guinea pig represents a model for evaluation of arrhythmogenic potential of drugs. It allows to evaluate complex reaction of cardiovascular system. Moreover, cardiac action potential of guinea pig is quite comparable to that of human and the results are thus well translatable to clinical medicine. Aim of this study was to evaluate arrhythmogenic potential of the TP-1, a new drug candidate with possible beta adrenergic action, in the model of anesthetised guinea pig. Methods: 24 guinea pigs were divided into three groups: positive control (esmolol, cardioselective beta-1 receptor blocker), negative control (vehiculum) and test group (TP-1). Animals were anesthetised by isoflurane, fixed to the heated pad, their neck and chest shaved, and jugular vein cannulated. ECG was recorded by needle electrodes subcutaneously fixed on the chest. Animal was stabilized. Then 4 doses of a drug or vehiculum were administered by rapid i.v. infusion. ECG and body temperature was recorded continually during the whole experiment using PowerLab (AD Instruments Ltd., CO, USA). ECG record was analysed using LabChart 8 Pro (AD Instruments Ltd., CO, USA). RR intervals and QT intervals were measured. QT was corrected according to Bazzet’s and Fridericia’s formulas. Incidence of arrhythmias was evaluated. Statistical analyses were performed in GraphPad Prism 5 (GraphPad Software, CA, USA). The results were compared between the groups and between particular doses within each group. Results: Dose-dependent changes of RR interval as well as QTc interval were observed in all groups. As expected according to in silico analyses, rapid onset of TP-1 effect and its short-term action on guinea pig ECG were observed in test group. Detailed results will be included in fulltext. Conclusion: Since effects of TP-1 has not been tested up to now, further experiments are needed to obtain deeper insights into its action on cardiac electrical activity.

Návaznosti

MUNI/A/1246/2020, interní kód MU

Název: Kardiovaskulární systém: od iontového kanálu k celotělovému modelu (Akronym: KAVASYKAMO)

Investor: Masarykova univerzita, Kardiovaskulární systém: od iontového kanálu k celotělovému modelu

MUNI/IGA/1098/2020, interní kód MU

Název: Arrhythmogenic potential of TP-1 in animal model

Investor: Masarykova univerzita, Arrhythmogenic potential of TP-1 in animal model

Citovat

BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ a Marie NOVÁKOVÁ. Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b. In 48. ročník konference "Computing in Cardiology". 2021.

@proceedings{1822797,
   author = {Bartáková, Anna and Stračina, Tibor and Opatřilová, Eva and Nováková, Marie},
   booktitle = {48. ročník konference "Computing in Cardiology"},
   keywords = {guinea pig, whole body model, beta-blocker, arrhythmogenic potential},
   language = {eng},
   title = {Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b},
   url = {https://ieeexplore.ieee.org/document/9662793},
   year = {2021}
}

TY  - CONF
ID  - 1822797
AU  - Bartáková, Anna - Stračina, Tibor - Opatřilová, Eva - Nováková, Marie
PY  - 2021
TI  - Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b
KW  - guinea pig, whole body model, beta-blocker, arrhythmogenic potential
UR  - https://ieeexplore.ieee.org/document/9662793
N2  - Introduction: Anesthetised guinea pig represents a model for evaluation of arrhythmogenic potential of drugs. It allows to evaluate complex reaction of cardiovascular system. Moreover, cardiac action potential of guinea pig is quite comparable to that of human and the results are thus well translatable to clinical medicine. Aim of this study was to evaluate arrhythmogenic potential of the TP-1, a new drug candidate with possible beta adrenergic action, in the model of anesthetised guinea pig. Methods: 24 guinea pigs were divided into three groups: positive control (esmolol, cardioselective beta-1 receptor blocker), negative control (vehiculum) and test group (TP-1). Animals were anesthetised by isoflurane, fixed to the heated pad, their neck and chest shaved, and jugular vein cannulated. ECG was recorded by needle electrodes subcutaneously fixed on the chest. Animal was stabilized. Then 4 doses of a drug or vehiculum were administered by rapid i.v. infusion. ECG and body temperature was recorded continually during the whole experiment using PowerLab (AD Instruments Ltd., CO, USA). ECG record was analysed using LabChart 8 Pro (AD Instruments Ltd., CO, USA). RR intervals and QT intervals were measured. QT was corrected according to Bazzet’s and Fridericia’s formulas. Incidence of arrhythmias was evaluated. Statistical analyses were performed in GraphPad Prism 5 (GraphPad Software, CA, USA). The results were compared between the groups and between particular doses within each group. Results: Dose-dependent changes of RR interval as well as QTc interval were observed in all groups. As expected according to in silico analyses, rapid onset of TP-1 effect and its short-term action on guinea pig ECG were observed in test group. Detailed results will be included in fulltext. Conclusion: Since effects of TP-1 has not been tested up to now, further experiments are needed to obtain deeper insights into its action on cardiac electrical activity.
ER  -

BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ a Marie NOVÁKOVÁ. Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b. In \textit{48. ročník konference ''Computing in Cardiology''}. 2021.

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