Guinea Pig ECG Changes under the Effect of New Drug Candidate
TP28b

k 2021

Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b

BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ and Marie NOVÁKOVÁ

Basic information

Original name

Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b

Name in Czech

Anestezované morče jako model pro testování nových léčiv

Authors

BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ and Marie NOVÁKOVÁ

Edition

48. ročník konference "Computing in Cardiology" 2021

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Medicine

Keywords (in Czech)

morče, celotělový model, beta-blokátor, arytmogenní potenciál

Keywords in English

guinea pig, whole body model, beta-blocker, arrhythmogenic potential

Abstract

V originále

Introduction: Anesthetised guinea pig represents a model for evaluation of arrhythmogenic potential of drugs. It allows to evaluate complex reaction of cardiovascular system. Moreover, cardiac action potential of guinea pig is quite comparable to that of human and the results are thus well translatable to clinical medicine. Aim of this study was to evaluate arrhythmogenic potential of the TP-1, a new drug candidate with possible beta adrenergic action, in the model of anesthetised guinea pig. Methods: 24 guinea pigs were divided into three groups: positive control (esmolol, cardioselective beta-1 receptor blocker), negative control (vehiculum) and test group (TP-1). Animals were anesthetised by isoflurane, fixed to the heated pad, their neck and chest shaved, and jugular vein cannulated. ECG was recorded by needle electrodes subcutaneously fixed on the chest. Animal was stabilized. Then 4 doses of a drug or vehiculum were administered by rapid i.v. infusion. ECG and body temperature was recorded continually during the whole experiment using PowerLab (AD Instruments Ltd., CO, USA). ECG record was analysed using LabChart 8 Pro (AD Instruments Ltd., CO, USA). RR intervals and QT intervals were measured. QT was corrected according to Bazzet’s and Fridericia’s formulas. Incidence of arrhythmias was evaluated. Statistical analyses were performed in GraphPad Prism 5 (GraphPad Software, CA, USA). The results were compared between the groups and between particular doses within each group. Results: Dose-dependent changes of RR interval as well as QTc interval were observed in all groups. As expected according to in silico analyses, rapid onset of TP-1 effect and its short-term action on guinea pig ECG were observed in test group. Detailed results will be included in fulltext. Conclusion: Since effects of TP-1 has not been tested up to now, further experiments are needed to obtain deeper insights into its action on cardiac electrical activity.

Links

MUNI/A/1246/2020, interní kód MU

Name: Kardiovaskulární systém: od iontového kanálu k celotělovému modelu (Acronym: KAVASYKAMO)

Investor: Masaryk University

MUNI/IGA/1098/2020, interní kód MU

Name: Arrhythmogenic potential of TP-1 in animal model

Investor: Masaryk University

Citovat

BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ and Marie NOVÁKOVÁ. Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b. In 48. ročník konference "Computing in Cardiology". 2021.

@proceedings{1822797,
   author = {Bartáková, Anna and Stračina, Tibor and Opatřilová, Eva and Nováková, Marie},
   booktitle = {48. ročník konference "Computing in Cardiology"},
   keywords = {guinea pig, whole body model, beta-blocker, arrhythmogenic potential},
   language = {eng},
   title = {Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b},
   url = {https://ieeexplore.ieee.org/document/9662793},
   year = {2021}
}

TY  - CONF
ID  - 1822797
AU  - Bartáková, Anna - Stračina, Tibor - Opatřilová, Eva - Nováková, Marie
PY  - 2021
TI  - Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b
KW  - guinea pig, whole body model, beta-blocker, arrhythmogenic potential
UR  - https://ieeexplore.ieee.org/document/9662793
N2  - Introduction: Anesthetised guinea pig represents a model for evaluation of arrhythmogenic potential of drugs. It allows to evaluate complex reaction of cardiovascular system. Moreover, cardiac action potential of guinea pig is quite comparable to that of human and the results are thus well translatable to clinical medicine. Aim of this study was to evaluate arrhythmogenic potential of the TP-1, a new drug candidate with possible beta adrenergic action, in the model of anesthetised guinea pig. Methods: 24 guinea pigs were divided into three groups: positive control (esmolol, cardioselective beta-1 receptor blocker), negative control (vehiculum) and test group (TP-1). Animals were anesthetised by isoflurane, fixed to the heated pad, their neck and chest shaved, and jugular vein cannulated. ECG was recorded by needle electrodes subcutaneously fixed on the chest. Animal was stabilized. Then 4 doses of a drug or vehiculum were administered by rapid i.v. infusion. ECG and body temperature was recorded continually during the whole experiment using PowerLab (AD Instruments Ltd., CO, USA). ECG record was analysed using LabChart 8 Pro (AD Instruments Ltd., CO, USA). RR intervals and QT intervals were measured. QT was corrected according to Bazzet’s and Fridericia’s formulas. Incidence of arrhythmias was evaluated. Statistical analyses were performed in GraphPad Prism 5 (GraphPad Software, CA, USA). The results were compared between the groups and between particular doses within each group. Results: Dose-dependent changes of RR interval as well as QTc interval were observed in all groups. As expected according to in silico analyses, rapid onset of TP-1 effect and its short-term action on guinea pig ECG were observed in test group. Detailed results will be included in fulltext. Conclusion: Since effects of TP-1 has not been tested up to now, further experiments are needed to obtain deeper insights into its action on cardiac electrical activity.
ER  -

BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ and Marie NOVÁKOVÁ. Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b. In \textit{48. ročník konference ''Computing in Cardiology''}. 2021.

Detailed Information on Publication Record