IIntravenous immunoglobulin modulates the number and function
of low density neutrophils in patients with CVID

a 2021

IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID

SLANINA, Peter; Julie ŠTÍCHOVÁ; Jiří LITZMAN; Zita CHOVANCOVÁ; Jan FRIČ et. al.

Basic information

Original name

IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID

Name in Czech

Intravenózní imunoglobuliny ovlivňují počet a funkci neutrofilů s nízkou denzitou u pacientů s CVID

Authors

SLANINA, Peter (703 Slovakia, belonging to the institution); Julie ŠTÍCHOVÁ (203 Czech Republic, belonging to the institution); Jiří LITZMAN (203 Czech Republic, belonging to the institution); Zita CHOVANCOVÁ (203 Czech Republic, belonging to the institution); Jan FRIČ (203 Czech Republic, belonging to the institution) and Marcela VLKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

11th International Conference Analytical Cytometry, 2021

Other information

Language

English

Type of outcome

Conference abstract

Field of Study

30102 Immunology

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

RIV identification code

RIV/00216224:14110/21:00123777

Organization unit

Faculty of Medicine

ISBN

978-80-88214-26-7

Keywords (in Czech)

Běžný variabilní imunodeficit; neutrofily s nízkou denzitou

Keywords in English

Common variable immunodeficiency; Low Density Neutrophils

Abstract

In the original language

Common variable immunodeficiency disorder (CVID) is the most common form of clinically significant primary immunodeficiency. Majority of patients receive intravenous immunoglobulin (IVIg) replacement therapy with potent immunomodulatory properties on individual components of the immune system. CVID is associated with chronic granulocytic activation and an increased percentage of Low Density Neutrophils (LDN) observed in the layer of peripheral blood mononuclear cells (PBMC). Changes in the percentage of LDN in PBMC and expression of their surface markers were studied in 25 CVID patients and 27 healthy donors (HD) in a whole blood sample and after in-vitro stimulation of whole blood with IVIg. We confirmed an increased number of LDN in the PBMC layer in a fresh blood sample from CVID patients. The LDN of patients and a HD group consisted of mature and immature neutrophils distinguished based on the expression of CD10 and CD16. Stimulation of whole blood with IVIg caused an increase of LDN in both CVID patients and HD. This enhancement in LDN was caused by mature neutrophils CD16+CD10+ in both groups. The suppressive effect of induced LDN on T-cell proliferation was confirmed. Flow cytometry measurements revealed altered expression of CD274 and CD10 on mature LDN of CVID patients. The addition of IVIg inhibited the expression of CD11b on the mature LDN in both experimental groups. These results indicate that IVIg supports the formation of LDN, which can negatively affect the immune response and can significantly contribute to the chronic inflammation observed in patients with CVID.

Links

MUNI/A/1244/2021, interní kód MU

Name: Vrozená imunita a její abnormality v rozvoji imunopatologických stavů

Investor: Masaryk University

Cite

SLANINA, Peter; Julie ŠTÍCHOVÁ; Jiří LITZMAN; Zita CHOVANCOVÁ; Jan FRIČ and Marcela VLKOVÁ. IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID (Intravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID). In 11th International Conference Analytical Cytometry. 2021. ISBN 978-80-88214-26-7.

@proceedings{1822819,
   author = {Slanina, Peter and Štíchová, Julie and Litzman, Jiří and Chovancová, Zita and Frič, Jan and Vlková, Marcela},
   booktitle = {11th International Conference Analytical Cytometry},
   keywords = {Common variable immunodeficiency; Low Density Neutrophils},
   language = {eng},
   isbn = {978-80-88214-26-7},
   title = {IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID},
   url = {https://www.conference.csac.cz/},
   year = {2021}
}

TY  - CONF
ID  - 1822819
AU  - Slanina, Peter - Štíchová, Julie - Litzman, Jiří - Chovancová, Zita - Frič, Jan - Vlková, Marcela
PY  - 2021
TI  - IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID
SN  - 9788088214267
KW  - Common variable immunodeficiency
KW  - Low Density Neutrophils
UR  - https://www.conference.csac.cz/
N2  - Common variable immunodeficiency disorder (CVID) is the most common form of clinically significant primary immunodeficiency. Majority of patients receive intravenous immunoglobulin (IVIg) replacement therapy with potent immunomodulatory properties on individual components of the immune system. CVID is associated with chronic granulocytic activation and an increased percentage of Low Density Neutrophils (LDN) observed in the layer of peripheral blood mononuclear cells (PBMC). Changes in the percentage of LDN in PBMC and expression of their surface markers were studied in 25 CVID patients and 27 healthy donors (HD) in a whole blood sample and after in-vitro stimulation of whole blood with IVIg. We confirmed an increased number of LDN in the PBMC layer in a fresh blood sample from CVID patients. The LDN of patients and a HD group consisted of mature and immature neutrophils distinguished based on the expression of CD10 and CD16. Stimulation of whole blood with IVIg caused an increase of LDN in both CVID patients and HD. This enhancement in LDN was caused by mature neutrophils CD16+CD10+ in both groups. The suppressive effect of induced LDN on T-cell proliferation was confirmed. Flow cytometry measurements revealed altered expression of CD274 and CD10 on mature LDN of CVID patients. The addition of IVIg inhibited the expression of CD11b on the mature LDN in both experimental groups. These results indicate that IVIg supports the formation of LDN, which can negatively affect the immune response and can significantly contribute to the chronic inflammation observed in patients with CVID.
ER  -

SLANINA, Peter; Julie ŠTÍCHOVÁ; Jiří LITZMAN; Zita CHOVANCOVÁ; Jan FRIČ and Marcela VLKOVÁ. IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID (Intravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID). In \textit{11th International Conference Analytical Cytometry}. 2021. ISBN~978-80-88214-26-7.

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