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@inproceedings{1823518,
author = {Assi, Navid and Pavelicova, Kristyna and Vaculovicova, Marketa and Vaculovič, Tomáš},
address = {Ostrava},
booktitle = {12th International Conference on Nanomaterials - Research and Application, NANOCON 2020},
doi = {http://dx.doi.org/10.37904/nanocon.2020.3752},
keywords = {Antibody; antigen; quantum dots; cleavage disulfide bonds; fluorescent},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Ostrava},
isbn = {978-80-87294-98-7},
pages = {395-398},
publisher = {TANGER Ltd.},
title = {Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection},
url = {https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection},
year = {2021}
}
TY - JOUR
ID - 1823518
AU - Assi, Navid - Pavelicova, Kristyna - Vaculovicova, Marketa - Vaculovič, Tomáš
PY - 2021
TI - Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection
PB - TANGER Ltd.
CY - Ostrava
SN - 9788087294987
KW - Antibody
KW - antigen
KW - quantum dots
KW - cleavage disulfide bonds
KW - fluorescent
UR - https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection
N2 - Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).
ER -
ASSI, Navid, Kristyna PAVELICOVA, Marketa VACULOVICOVA a Tomáš VACULOVIČ. Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection. Online. In \textit{12th International Conference on Nanomaterials - Research and Application, NANOCON 2020}. Ostrava: TANGER Ltd., 2021, s.~395-398. ISBN~978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3752.
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