DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for
mouse preimplantation embryo development and cell-fate
specification

J 2021

DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification

BORA, Pablo, Lenka GAHUROVÁ, Andrea HAUSEROVÁ, Martina STIBOROVÁ, Collier REBECCA et. al.

Základní údaje

Originální název

DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification

Autoři

BORA, Pablo (203 Česká republika), Lenka GAHUROVÁ (203 Česká republika), Andrea HAUSEROVÁ (203 Česká republika), Martina STIBOROVÁ (203 Česká republika), Collier REBECCA (203 Česká republika), David POTĚŠIL (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí) a Alexander W. BRUCE

Vydání

Open Biology, 2021, 2046-2441

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 7.124

Kód RIV

RIV/00216224:14740/21:00123878

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1098/rsob.210092

UT WoS

000674669000001

Klíčová slova anglicky

DDX21; p38-MAPK; preimplantation embryo development; cell fate specification

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 10. 2024 14:34, Ing. Martina Blahová

Anotace

V originále

Successful navigation of the mouse preimplantation stages of development, during which three distinct blastocyst lineages are derived, represents a prerequisite for continued development. We previously identified a role for p38-mitogen-activated kinases (p38-MAPK) regulating blastocyst inner cell mass (ICM) cell fate, specifically primitive endoderm (PrE) differentiation, that is intimately linked to rRNA precursor processing, polysome formation and protein translation regulation. Here, we develop this work by assaying the role of DEAD-box RNA helicase 21 (DDX21), a known regulator of rRNA processing, in the context of p38-MAPK regulation of preimplantation mouse embryo development. We show nuclear DDX21 protein is robustly expressed from the 16-cell stage, becoming exclusively nucleolar during blastocyst maturation, a localization dependent on active p38-MAPK. siRNA-mediated clonal Ddx21 knockdown within developing embryos is associated with profound cell-autonomous and non-autonomous proliferation defects and reduced blastocyst volume, by the equivalent peri-implantation blastocyst stage. Moreover, ICM residing Ddx21 knockdown clones express the EPI marker NANOG but rarely express the PrE differentiation marker GATA4. These data contribute further significance to the emerging importance of lineage-specific translation regulation, as identified for p38-MAPK, during mouse preimplantation development.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LM2018129, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

BORA, Pablo, Lenka GAHUROVÁ, Andrea HAUSEROVÁ, Martina STIBOROVÁ, Collier REBECCA, David POTĚŠIL, Zbyněk ZDRÁHAL a Alexander W. BRUCE. DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification. Open Biology. 2021, roč. 11, č. 7, s. "210092", 15 s. ISSN 2046-2441. Dostupné z: https://dx.doi.org/10.1098/rsob.210092.

@article{1824657,
   author = {Bora, Pablo and Gahurová, Lenka and Hauserová, Andrea and Stiborová, Martina and Rebecca, Collier and Potěšil, David and Zdráhal, Zbyněk and Bruce, Alexander W.},
   article_number = {7},
   doi = {http://dx.doi.org/10.1098/rsob.210092},
   keywords = {DDX21; p38-MAPK; preimplantation embryo development; cell fate specification},
   language = {eng},
   issn = {2046-2441},
   journal = {Open Biology},
   title = {DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification},
   url = {https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.210092},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1824657
AU  - Bora, Pablo - Gahurová, Lenka - Hauserová, Andrea - Stiborová, Martina - Rebecca, Collier - Potěšil, David - Zdráhal, Zbyněk - Bruce, Alexander W.
PY  - 2021
TI  - DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification
JF  - Open Biology
VL  - 11
IS  - 7
SP  - "210092"
EP  - "210092"
SN  - 20462441
KW  - DDX21
KW  - p38-MAPK
KW  - preimplantation embryo development
KW  - cell fate specification
UR  - https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.210092
N2  - Successful navigation of the mouse preimplantation stages of development, during which three distinct blastocyst lineages are derived, represents a prerequisite for continued development. We previously identified a role for p38-mitogen-activated kinases (p38-MAPK) regulating blastocyst inner cell mass (ICM) cell fate, specifically primitive endoderm (PrE) differentiation, that is intimately linked to rRNA precursor processing, polysome formation and protein translation regulation. Here, we develop this work by assaying the role of DEAD-box RNA helicase 21 (DDX21), a known regulator of rRNA processing, in the context of p38-MAPK regulation of preimplantation mouse embryo development. We show nuclear DDX21 protein is robustly expressed from the 16-cell stage, becoming exclusively nucleolar during blastocyst maturation, a localization dependent on active p38-MAPK. siRNA-mediated clonal Ddx21 knockdown within developing embryos is associated with profound cell-autonomous and non-autonomous proliferation defects and reduced blastocyst volume, by the equivalent peri-implantation blastocyst stage. Moreover, ICM residing Ddx21 knockdown clones express the EPI marker NANOG but rarely express the PrE differentiation marker GATA4. These data contribute further significance to the emerging importance of lineage-specific translation regulation, as identified for p38-MAPK, during mouse preimplantation development.
ER  -

BORA, Pablo, Lenka GAHUROVÁ, Andrea HAUSEROVÁ, Martina STIBOROVÁ, Collier REBECCA, David POTĚŠIL, Zbyněk ZDRÁHAL a Alexander W. BRUCE. DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification. \textit{Open Biology}. 2021, roč.~11, č.~7, s.~''210092'', 15 s. ISSN~2046-2441. Dostupné z: https://dx.doi.org/10.1098/rsob.210092.

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