p38-MAPK-mediated translation regulation during early
blastocyst development is required for primitive endoderm
differentiation in mice

J 2021

p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice

BORA, Pablo; Lenka GAHUROVÁ; Tomáš MAŠEK; Andrea HAUSEROVÁ; David POTĚŠIL et. al.

Základní údaje

Originální název

p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice

Autoři

BORA, Pablo (203 Česká republika); Lenka GAHUROVÁ (203 Česká republika); Tomáš MAŠEK (203 Česká republika); Andrea HAUSEROVÁ (203 Česká republika); David POTĚŠIL (203 Česká republika, domácí); Denisa JANSOVÁ (203 Česká republika); Andrej SUSOR; Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí); Anna AJDUK (616 Polsko); Martin POSPÍŠEK (203 Česká republika) a Alexander W. BRUCE

Vydání

Communications Biology, 2021, 2399-3642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10700 1.7 Other natural sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 6.548

Kód RIV

RIV/00216224:14740/21:00123879

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/s42003-021-02290-z

UT WoS

000668739100001

EID Scopus

2-s2.0-85111784798

Klíčová slova anglicky

ACTIVATED PROTEIN-KINASE; INNER CELL MASS; MAP KINASE; PREIMPLANTATION EMBRYO; MESSENGER-RNA; NA/K-ATPASE; 1A MYBBP1A; P38 MAPK; MOUSE; GROWTH

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:46, Ing. Martina Blahová

Anotace

V originále

Successful specification of the two mouse blastocyst inner cell mass (ICM) lineages (the primitive endoderm (PrE) and epiblast) is a prerequisite for continued development and requires active fibroblast growth factor 4 (FGF4) signaling. Previously, we identified a role for p38 mitogen-activated protein kinases (p38-MAPKs) during PrE differentiation, but the underlying mechanisms have remained unresolved. Here, we report an early blastocyst window of p38-MAPK activity that is required to regulate ribosome-related gene expression, rRNA precursor processing, polysome formation and protein translation. We show that p38-MAPK inhibition-induced PrE phenotypes can be partially rescued by activating the translational regulator mTOR. However, similar PrE phenotypes associated with extracellular signal-regulated kinase (ERK) pathway inhibition targeting active FGF4 signaling are not affected by mTOR activation. These data indicate a specific role for p38-MAPKs in providing a permissive translational environment during mouse blastocyst PrE differentiation that is distinct from classically reported FGF4-based mechanisms. Bora et al. show that an early blastocyst window of p38-MAPK activity regulates ribosome-related gene expression, rRNA precursor processing, polysome formation, and protein translation. This study suggests a distinct role of p38-MAPKs for providing a permissive translational environment during mouse blastocyst primitive endoderm differentiation.

Návaznosti

LM2018129, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

BORA, Pablo; Lenka GAHUROVÁ; Tomáš MAŠEK; Andrea HAUSEROVÁ; David POTĚŠIL; Denisa JANSOVÁ; Andrej SUSOR; Zbyněk ZDRÁHAL; Anna AJDUK; Martin POSPÍŠEK a Alexander W. BRUCE. p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice. Communications Biology. 2021, roč. 4, č. 1, s. "788", 19 s. ISSN 2399-3642. Dostupné z: https://dx.doi.org/10.1038/s42003-021-02290-z.

@article{1824697,
   author = {Bora, Pablo and Gahurová, Lenka and Mašek, Tomáš and Hauserová, Andrea and Potěšil, David and Jansová, Denisa and Susor, Andrej and Zdráhal, Zbyněk and Ajduk, Anna and Pospíšek, Martin and Bruce, Alexander W.},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s42003-021-02290-z},
   keywords = {ACTIVATED PROTEIN-KINASE; INNER CELL MASS; MAP KINASE; PREIMPLANTATION EMBRYO; MESSENGER-RNA; NA/K-ATPASE; 1A MYBBP1A; P38 MAPK; MOUSE; GROWTH},
   language = {eng},
   issn = {2399-3642},
   journal = {Communications Biology},
   title = {p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice},
   url = {https://www.nature.com/articles/s42003-021-02290-z.pdf},
   volume = {4},
   year = {2021}
}

TY  - JOUR
ID  - 1824697
AU  - Bora, Pablo - Gahurová, Lenka - Mašek, Tomáš - Hauserová, Andrea - Potěšil, David - Jansová, Denisa - Susor, Andrej - Zdráhal, Zbyněk - Ajduk, Anna - Pospíšek, Martin - Bruce, Alexander W.
PY  - 2021
TI  - p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice
JF  - Communications Biology
VL  - 4
IS  - 1
SP  - "788"
EP  - "788"
SN  - 23993642
KW  - ACTIVATED PROTEIN-KINASE
KW  - INNER CELL MASS
KW  - MAP KINASE
KW  - PREIMPLANTATION EMBRYO
KW  - MESSENGER-RNA
KW  - NA/K-ATPASE
KW  - 1A MYBBP1A
KW  - P38 MAPK
KW  - MOUSE
KW  - GROWTH
UR  - https://www.nature.com/articles/s42003-021-02290-z.pdf
N2  - Successful specification of the two mouse blastocyst inner cell mass (ICM) lineages (the primitive endoderm (PrE) and epiblast) is a prerequisite for continued development and requires active fibroblast growth factor 4 (FGF4) signaling. Previously, we identified a role for p38 mitogen-activated protein kinases (p38-MAPKs) during PrE differentiation, but the underlying mechanisms have remained unresolved. Here, we report an early blastocyst window of p38-MAPK activity that is required to regulate ribosome-related gene expression, rRNA precursor processing, polysome formation and protein translation. We show that p38-MAPK inhibition-induced PrE phenotypes can be partially rescued by activating the translational regulator mTOR. However, similar PrE phenotypes associated with extracellular signal-regulated kinase (ERK) pathway inhibition targeting active FGF4 signaling are not affected by mTOR activation. These data indicate a specific role for p38-MAPKs in providing a permissive translational environment during mouse blastocyst PrE differentiation that is distinct from classically reported FGF4-based mechanisms. Bora et al. show that an early blastocyst window of p38-MAPK activity regulates ribosome-related gene expression, rRNA precursor processing, polysome formation, and protein translation. This study suggests a distinct role of p38-MAPKs for providing a permissive translational environment during mouse blastocyst primitive endoderm differentiation.
ER  -

BORA, Pablo; Lenka GAHUROVÁ; Tomáš MAŠEK; Andrea HAUSEROVÁ; David POTĚŠIL; Denisa JANSOVÁ; Andrej SUSOR; Zbyněk ZDRÁHAL; Anna AJDUK; Martin POSPÍŠEK a Alexander W. BRUCE. p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice. \textit{Communications Biology}. 2021, roč.~4, č.~1, s.~''788'', 19 s. ISSN~2399-3642. Dostupné z: https://dx.doi.org/10.1038/s42003-021-02290-z.

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