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@article{1824758,
author = {Popovič, Mikuláš and Liu, Yiling and Lattová, Erika and Mann, Dean and Curreli, Sabrina and Zdráhal, Zbyněk and Edelman, Martin and Bryant, Joseph},
article_number = {3},
doi = {http://dx.doi.org/10.4149/neo_2021_200906N953},
keywords = {cell-based model of LAC; lung adenocarcinoma; growth pattern conversion; OCT-4; SOX-2; NANOG; pluripotent genes},
language = {eng},
issn = {0028-2685},
journal = {Neoplasma},
title = {In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion},
url = {http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7102&category_id=172&option=com_virtuemart&vmcchk=1&Itemid=1},
volume = {68},
year = {2021}
}
TY - JOUR
ID - 1824758
AU - Popovič, Mikuláš - Liu, Yiling - Lattová, Erika - Mann, Dean - Curreli, Sabrina - Zdráhal, Zbyněk - Edelman, Martin - Bryant, Joseph
PY - 2021
TI - In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion
JF - Neoplasma
VL - 68
IS - 3
SP - 498-508
EP - 498-508
PB - Slovenská akademie vied
SN - 00282685
KW - cell-based model of LAC
KW - lung adenocarcinoma
KW - growth pattern conversion
KW - OCT-4
KW - SOX-2
KW - NANOG
KW - pluripotent genes
UR - http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7102&category_id=172&option=com_virtuemart&vmcchk=1&Itemid=1
N2 - Lung adenocarcinoma (LAC) is a common and aggressive form of lung cancer that is increasing in incidence among never smokers at a younger age. Current treatment of patients with LAC is insufficient and there is a need for identification of effective biomarkers and development of therapeutic targets. These demands require also unproved models for in vivo and in vitro experimentation. In this study, we describe the establishment of two LAC cell lines, named LuCa-3 and LuCa-6. Both were derived from pleural effusion (PE) cells of LAC patients (13 and 16) and readily propagated as tumor xenografts in immunodeficient mice. PE cells from the patient L6 exhibited also the capacity for in vitro growth and were cultured in two forms: (i) as a suspension growing cell population, labeled LuCa-6S, composed of non-clumping single cells; and (ii) as a monolayer-like culture, labeled LuCa-6A, exhibiting tight cell-to-cell and to culture surface adherence. Unique features of these two sublines and their cell clones are the capacity to convert from a non-clumping single-cell suspension into the adherent growth pattern and vice versa. Immunostaining of patients' tumor tissue xenografts and cultured subline cells displayed markers specific for the phenotype of human LAC. LuCa-6S and LuCa-6A cells did not reveal a noticeable disparity in quantitative growth characteristics. However, a number of differences were detected between these two cell populations manifested in detection or intensities of antigen expressions on the cell surface (CD133, SFTPC) and in the nucleus (TTF-1) including pluripotcnt (OCT-4, SOX-2, NANOG) genes in cancer stem-like cells (CSCs). Dissimilarities between these two sublines were also detected in N-glycan profiles and in the sensitivity to natural killer cells. Salient features of these subline cell populations are responsiveness to selective upregulation of the pluripotent genes in subsets of CSCs via conversion of their growth patterns and/or by using culture stem media with growth factors. The described in vivo/in vitro model enables broader experimental approaches in studies of lung adenocarcinoma.
ER -
POPOVIČ, Mikuláš, Yiling LIU, Erika LATTOVÁ, Dean MANN, Sabrina CURRELI, Zbyněk ZDRÁHAL, Martin EDELMAN and Joseph BRYANT. In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion. \textit{Neoplasma}. Slovenská akademie vied, 2021, vol.~68, No~3, p.~498-508. ISSN~0028-2685. Available from: https://dx.doi.org/10.4149/neo\_{}2021\_{}200906N953.
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