Comparative analyses of angiosperm secretomes identify
apoplastic pollen tube functions and novel secreted peptides

J 2021

Comparative analyses of angiosperm secretomes identify apoplastic pollen tube functions and novel secreted peptides

FLORES-TORNERO, Maria, Lele WANG, David POTĚŠIL, Said HAFIDH, Frank VOGLER et. al.

Základní údaje

Originální název

Comparative analyses of angiosperm secretomes identify apoplastic pollen tube functions and novel secreted peptides

Autoři

FLORES-TORNERO, Maria (276 Německo), Lele WANG (203 Česká republika), David POTĚŠIL (203 Česká republika, domácí), Said HAFIDH (203 Česká republika), Frank VOGLER (276 Německo), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí), David HONYS (203 Česká republika), Stefanie SPRUNCK (276 Německo) a Thomas DRESSELHAUS (276 Německo)

Vydání

Plant Reproduction, 2021, 2194-7953

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 4.217

Kód RIV

RIV/00216224:14740/21:00123882

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s00497-020-00399-5

UT WoS

000594809800001

Klíčová slova anglicky

Pollen tube; Proteomics; Secretome; Cell wall; CRP; Signaling; Amborella; Maize; Tobacco

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 10. 2024 14:11, Ing. Martina Blahová

Anotace

V originále

Key message Analyses of secretomes of in vitro grown pollen tubes from Amborella, maize and tobacco identified many components of processes associated with the cell wall, signaling and metabolism as well as novel small secreted peptides. Flowering plants (angiosperms) generate pollen grains that germinate on the stigma and produce tubes to transport their sperm cells cargo deep into the maternal reproductive tissues toward the ovules for a double fertilization process. During their journey, pollen tubes secrete many proteins (secreted proteome or secretome) required, for example, for communication with the maternal reproductive tissues, to build a solid own cell wall that withstands their high turgor pressure while softening simultaneously maternal cell wall tissue. The composition and species specificity or family specificity of the pollen tube secretome is poorly understood. Here, we provide a suitable method to obtain the pollen tube secretome from in vitro grown pollen tubes of the basal angiosperm Amborella trichopoda (Amborella) and the Poaceae model maize. The previously published secretome of tobacco pollen tubes was used as an example of eudicotyledonous plants in this comparative study. The secretome of the three species is each strongly different compared to the respective protein composition of pollen grains and tubes. In Amborella and maize, about 40% proteins are secreted by the conventional "classic" pathway and 30% by unconventional pathways. The latter pathway is expanded in tobacco. Proteins enriched in the secretome are especially involved in functions associated with the cell wall, cell surface, energy and lipid metabolism, proteolysis and redox processes. Expansins, pectin methylesterase inhibitors and RALFs are enriched in maize, while tobacco secretes many proteins involved, for example, in proteolysis and signaling. While the majority of proteins detected in the secretome occur also in pollen grains and pollen tubes, and correlate in the number of mapped peptides with relative gene expression levels, some novel secreted small proteins were identified. Moreover, the identification of secreted proteins containing pro-peptides indicates that these are processed in the apoplast. In conclusion, we provide a proteome resource from three distinct angiosperm clades that can be utilized among others to study the localization, abundance and processing of known secreted proteins and help to identify novel pollen tube secreted proteins for functional studies.

Návaznosti

EF16_019/0000738, projekt VaV

Název: Centrum experimentální biologie rostlin

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

FLORES-TORNERO, Maria, Lele WANG, David POTĚŠIL, Said HAFIDH, Frank VOGLER, Zbyněk ZDRÁHAL, David HONYS, Stefanie SPRUNCK a Thomas DRESSELHAUS. Comparative analyses of angiosperm secretomes identify apoplastic pollen tube functions and novel secreted peptides. Plant Reproduction. 2021, roč. 34, č. 1, s. 47-60. ISSN 2194-7953. Dostupné z: https://dx.doi.org/10.1007/s00497-020-00399-5.

@article{1824763,
   author = {FloresandTornero, Maria and Wang, Lele and Potěšil, David and Hafidh, Said and Vogler, Frank and Zdráhal, Zbyněk and Honys, David and Sprunck, Stefanie and Dresselhaus, Thomas},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s00497-020-00399-5},
   keywords = {Pollen tube; Proteomics; Secretome; Cell wall; CRP; Signaling; Amborella; Maize; Tobacco},
   language = {eng},
   issn = {2194-7953},
   journal = {Plant Reproduction},
   title = {Comparative analyses of angiosperm secretomes identify apoplastic pollen tube functions and novel secreted peptides},
   url = {https://link.springer.com/article/10.1007/s00497-020-00399-5},
   volume = {34},
   year = {2021}
}

TY  - JOUR
ID  - 1824763
AU  - Flores-Tornero, Maria - Wang, Lele - Potěšil, David - Hafidh, Said - Vogler, Frank - Zdráhal, Zbyněk - Honys, David - Sprunck, Stefanie - Dresselhaus, Thomas
PY  - 2021
TI  - Comparative analyses of angiosperm secretomes identify apoplastic pollen tube functions and novel secreted peptides
JF  - Plant Reproduction
VL  - 34
IS  - 1
SP  - 47-60
EP  - 47-60
SN  - 21947953
KW  - Pollen tube
KW  - Proteomics
KW  - Secretome
KW  - Cell wall
KW  - CRP
KW  - Signaling
KW  - Amborella
KW  - Maize
KW  - Tobacco
UR  - https://link.springer.com/article/10.1007/s00497-020-00399-5
N2  - Key message Analyses of secretomes of in vitro grown pollen tubes from Amborella, maize and tobacco identified many components of processes associated with the cell wall, signaling and metabolism as well as novel small secreted peptides. Flowering plants (angiosperms) generate pollen grains that germinate on the stigma and produce tubes to transport their sperm cells cargo deep into the maternal reproductive tissues toward the ovules for a double fertilization process. During their journey, pollen tubes secrete many proteins (secreted proteome or secretome) required, for example, for communication with the maternal reproductive tissues, to build a solid own cell wall that withstands their high turgor pressure while softening simultaneously maternal cell wall tissue. The composition and species specificity or family specificity of the pollen tube secretome is poorly understood. Here, we provide a suitable method to obtain the pollen tube secretome from in vitro grown pollen tubes of the basal angiosperm Amborella trichopoda (Amborella) and the Poaceae model maize. The previously published secretome of tobacco pollen tubes was used as an example of eudicotyledonous plants in this comparative study. The secretome of the three species is each strongly different compared to the respective protein composition of pollen grains and tubes. In Amborella and maize, about 40% proteins are secreted by the conventional "classic" pathway and 30% by unconventional pathways. The latter pathway is expanded in tobacco. Proteins enriched in the secretome are especially involved in functions associated with the cell wall, cell surface, energy and lipid metabolism, proteolysis and redox processes. Expansins, pectin methylesterase inhibitors and RALFs are enriched in maize, while tobacco secretes many proteins involved, for example, in proteolysis and signaling. While the majority of proteins detected in the secretome occur also in pollen grains and pollen tubes, and correlate in the number of mapped peptides with relative gene expression levels, some novel secreted small proteins were identified. Moreover, the identification of secreted proteins containing pro-peptides indicates that these are processed in the apoplast. In conclusion, we provide a proteome resource from three distinct angiosperm clades that can be utilized among others to study the localization, abundance and processing of known secreted proteins and help to identify novel pollen tube secreted proteins for functional studies.
ER  -

FLORES-TORNERO, Maria, Lele WANG, David POTĚŠIL, Said HAFIDH, Frank VOGLER, Zbyněk ZDRÁHAL, David HONYS, Stefanie SPRUNCK a Thomas DRESSELHAUS. Comparative analyses of angiosperm secretomes identify apoplastic pollen tube functions and novel secreted peptides. \textit{Plant Reproduction}. 2021, roč.~34, č.~1, s.~47-60. ISSN~2194-7953. Dostupné z: https://dx.doi.org/10.1007/s00497-020-00399-5.

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