14-3-3ζ phosphomimicking mutants: an alternative to
phosphorylation?

D 2021

14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?

NÁPLAVOVÁ, Alexandra, Aneta KOZELEKOVÁ a Jozef HRITZ

Základní údaje

Originální název

14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?

Autoři

NÁPLAVOVÁ, Alexandra (203 Česká republika, domácí), Aneta KOZELEKOVÁ (203 Česká republika, domácí) a Jozef HRITZ (703 Slovensko, garant, domácí)

Vydání

Bratislava, Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov, od s. 703-708, 6 s. 2021

Nakladatel

Univerzita Komenského v Bratislave

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-223-5132-4

Klíčová slova anglicky

14-3-3; 14-3-3zeta; phosphomimicking mutant; monomerization; thermal stability

Štítky

rivok

Příznaky

Recenzováno

Změněno: 16. 5. 2024 09:24, Mgr. Eva Dubská

Anotace

V originále

The 14-3-3 proteins are typically found in dimeric form crucial for their proper function in cells. Yet, a unique role of monomers has been debated in recent studies. Monomerization of 14-3-3ζ is regulated via phosphorylation of Ser58 located at dimer interface. However, it is difficult to obtain 14-3-3ζ specifically phosphorylated at Ser58 (pS58) in sufficient purity and amount. For these reasons three distinct phosphomimicking mutants, i. e. 14-3-3ζ S58E, S58D and S57D S58D were designed and studied in terms of the oligomeric nature and thermodynamical properties. Finally, they were compared to 14-3-3ζ wild type and monomeric mutant L12E M78K, used to approximate monomeric 14-3-3ζ pS58, to discuss whether they could be valid alternative for study of 14-3-3ζ pS58. Although phosphomimicking mutants S58E and S58D and 14-3-3ζ pS58 share negative charge at position 58, they were observed to exhibit distinct properties than monomeric 14-3-3ζ. Designed double mutant S57D S58D with additional negative charge in the Ser58 surrounding appeared as more appropriate alternative of pS58 protein.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LTAUSA18168, projekt VaV

Název: Selektivní NMR značení jako nástroj pro charakterizaci proteinových komplexů zapojených do neurodegenerativních onemocnění

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Selektivní NMR značení jako nástroj pro charakterizaci proteinových komplexů zapojených do neurodegenerativních onemocnění, INTER-ACTION

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

NÁPLAVOVÁ, Alexandra, Aneta KOZELEKOVÁ a Jozef HRITZ. 14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?. Online. In Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov. Bratislava: Univerzita Komenského v Bratislave, 2021, s. 703-708. ISBN 978-80-223-5132-4.

@inproceedings{1825177,
   author = {Náplavová, Alexandra and Kozeleková, Aneta and Hritz, Jozef},
   address = {Bratislava},
   booktitle = {Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov},
   keywords = {14-3-3; 14-3-3zeta; phosphomimicking mutant; monomerization; thermal stability},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Bratislava},
   isbn = {978-80-223-5132-4},
   pages = {703-708},
   publisher = {Univerzita Komenského v Bratislave},
   title = {14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?},
   url = {https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf},
   year = {2021}
}

TY  - JOUR
ID  - 1825177
AU  - Náplavová, Alexandra - Kozeleková, Aneta - Hritz, Jozef
PY  - 2021
TI  - 14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?
PB  - Univerzita Komenského v Bratislave
CY  - Bratislava
SN  - 9788022351324
KW  - 14-3-3
KW  - 14-3-3zeta
KW  - phosphomimicking mutant
KW  - monomerization
KW  - thermal stability
UR  - https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf
N2  - The 14-3-3 proteins are typically found in dimeric form crucial for their proper function in cells. Yet, a unique role of monomers has been debated in recent studies. Monomerization of 14-3-3ζ is regulated via phosphorylation of Ser58 located at dimer interface. However, it is difficult to obtain 14-3-3ζ specifically phosphorylated at Ser58 (pS58) in sufficient purity and amount. For these reasons three distinct phosphomimicking mutants, i. e. 14-3-3ζ S58E, S58D and S57D S58D were designed and studied in terms of the oligomeric nature and thermodynamical properties. Finally, they were compared to 14-3-3ζ wild type and monomeric mutant L12E M78K, used to approximate monomeric 14-3-3ζ pS58, to discuss whether they could be valid alternative for study of 14-3-3ζ pS58. Although phosphomimicking mutants S58E and S58D and 14-3-3ζ pS58 share negative charge at position 58, they were observed to exhibit distinct properties than monomeric 14-3-3ζ. Designed double mutant S57D S58D with additional negative charge in the Ser58 surrounding appeared as more appropriate alternative of pS58 protein.
ER  -

NÁPLAVOVÁ, Alexandra, Aneta KOZELEKOVÁ a Jozef HRITZ. 14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?. Online. In \textit{Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov}. Bratislava: Univerzita Komenského v Bratislave, 2021, s.~703-708. ISBN~978-80-223-5132-4.

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