Další formáty:
BibTeX
LaTeX
RIS
@inproceedings{1825177,
author = {Náplavová, Alexandra and Kozeleková, Aneta and Hritz, Jozef},
address = {Bratislava},
booktitle = {Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov},
keywords = {14-3-3; 14-3-3zeta; phosphomimicking mutant; monomerization; thermal stability},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Bratislava},
isbn = {978-80-223-5132-4},
pages = {703-708},
publisher = {Univerzita Komenského v Bratislave},
title = {14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?},
url = {https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf},
year = {2021}
}
TY - JOUR
ID - 1825177
AU - Náplavová, Alexandra - Kozeleková, Aneta - Hritz, Jozef
PY - 2021
TI - 14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?
PB - Univerzita Komenského v Bratislave
CY - Bratislava
SN - 9788022351324
KW - 14-3-3
KW - 14-3-3zeta
KW - phosphomimicking mutant
KW - monomerization
KW - thermal stability
UR - https://fns.uniba.sk/fileadmin/prif/svk/zborniky/Zbornik2021.pdf
N2 - The 14-3-3 proteins are typically found in dimeric form crucial for their proper function in cells. Yet, a unique role of monomers has been debated in recent studies. Monomerization of 14-3-3ζ is regulated via phosphorylation of Ser58 located at dimer interface. However, it is difficult to obtain 14-3-3ζ specifically phosphorylated at Ser58 (pS58) in sufficient purity and amount. For these reasons three distinct phosphomimicking mutants, i. e. 14-3-3ζ S58E, S58D and S57D S58D were designed and studied in terms of the oligomeric nature and thermodynamical properties. Finally, they were compared to 14-3-3ζ wild type and monomeric mutant L12E M78K, used to approximate monomeric 14-3-3ζ pS58, to discuss whether they could be valid alternative for study of 14-3-3ζ pS58. Although phosphomimicking mutants S58E and S58D and 14-3-3ζ pS58 share negative charge at position 58, they were observed to exhibit distinct properties than monomeric 14-3-3ζ. Designed double mutant S57D S58D with additional negative charge in the Ser58 surrounding appeared as more appropriate alternative of pS58 protein.
ER -
NÁPLAVOVÁ, Alexandra, Aneta KOZELEKOVÁ a Jozef HRITZ. 14-3-3ζ phosphomimicking mutants: an alternative to phosphorylation?. Online. In \textit{Študentská vedecká konferencia PriF UK 2021: zborník recenzovaných príspevkov}. Bratislava: Univerzita Komenského v Bratislave, 2021, s.~703-708. ISBN~978-80-223-5132-4.
|
