Structure of Pseudomonas aeruginosa infecting bacteriophage
JBD30 solved by cryo-electron microscopy

a 2021

Structure of Pseudomonas aeruginosa infecting bacteriophage JBD30 solved by cryo-electron microscopy

VALENTOVÁ, Lucie, Tibor FÜZIK a Pavel PLEVKA

Základní údaje

Originální název

Structure of Pseudomonas aeruginosa infecting bacteriophage JBD30 solved by cryo-electron microscopy

Autoři

VALENTOVÁ, Lucie (203 Česká republika, domácí), Tibor FÜZIK (703 Slovensko, domácí) a Pavel PLEVKA (203 Česká republika, garant, domácí)

Vydání

1st Student Conference in Structural Biology, 2021

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/21:00123923

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Pseudomonas aeruginosa; bacteriophage JBD30; antibiotic resistance; cryo-electron microscopy; cryo-electron tomography

Štítky

rivok

Změněno: 24. 1. 2022 09:34, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Pseudomonas aeruginosa is an opportunistic human pathogen that causes acute and chronic infections, which can lead to life-threating septic shock. Treatment of these infections is further complicated by the ability of these bacteria to form biofilm. Biofilm serves as a shield through which the antibiotics cannot pass. Unlike antibiotics, bacteriophages are able to penetrate throug h biofilm, infect and kill bacterial cells. Here we present the structure of a bacteriophage JBD30 virion resolved by cryo- electron microscopy. Bacteriophage JBD30 belongs to the family Siphoviridae, order Caudovirales. Its virion is composed of non-enveloped icosahedral head of 60 nm in diameter connected via dodecameric portal with a non-contractile 180 nm long flexible tail. JBD30 tail is terminated with a baseplate embellished with side-tail fibres. Bacteriophage head is built from 415 copies of HK -97 l ike major capsid protein and further decorated on three-fold and quasi-three-fold axis with trimers of minor capsid protein. Data from cryo-electron tomography revealed that bacteriophage JBD30 uses the baseplate side-tail fibers for the attachment to its prey – bacterium Pseudomonas aeruginosa. P. aeruginosa pili type IV, that grow only from bacterial cell pole, serve as a primary receptor for the bacteriophage JBD30. After binding to pili, phage is pulled towards the cell surface by pili retraction, injects its nucleic acid into the bacterium and starts its replication cycle.

Návaznosti

LL1906, projekt VaV

Název: Replikace fágů v bakteriálním biofilmu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Replikace fágů v bakteriálním biofilmu

Citovat

VALENTOVÁ, Lucie, Tibor FÜZIK a Pavel PLEVKA. Structure of Pseudomonas aeruginosa infecting bacteriophage JBD30 solved by cryo-electron microscopy. In 1st Student Conference in Structural Biology. 2021.

@proceedings{1826243,
   author = {Valentová, Lucie and Füzik, Tibor and Plevka, Pavel},
   booktitle = {1st Student Conference in Structural Biology},
   keywords = {Pseudomonas aeruginosa; bacteriophage JBD30; antibiotic resistance; cryo-electron microscopy; cryo-electron tomography},
   language = {eng},
   title = {Structure of Pseudomonas aeruginosa infecting bacteriophage JBD30 solved by cryo-electron microscopy},
   url = {https://cssb.structbio.org/wp-content/uploads/1stSCSB_booklet_fin.pdf},
   year = {2021}
}

TY  - CONF
ID  - 1826243
AU  - Valentová, Lucie - Füzik, Tibor - Plevka, Pavel
PY  - 2021
TI  - Structure of Pseudomonas aeruginosa infecting bacteriophage JBD30 solved by cryo-electron microscopy
KW  - Pseudomonas aeruginosa
KW  - bacteriophage JBD30
KW  - antibiotic resistance
KW  - cryo-electron microscopy
KW  - cryo-electron tomography
UR  - https://cssb.structbio.org/wp-content/uploads/1stSCSB_booklet_fin.pdf
N2  - Pseudomonas aeruginosa is an opportunistic human pathogen that causes acute and chronic infections, which can lead to life-threating septic shock. Treatment of these infections is further complicated by the ability of these bacteria to form biofilm. Biofilm serves as a shield through which the antibiotics cannot pass. Unlike antibiotics, bacteriophages are able to penetrate throug h biofilm, infect and kill bacterial cells. Here we present the structure of a bacteriophage JBD30 virion resolved by cryo- electron microscopy. Bacteriophage JBD30 belongs to the family Siphoviridae, order Caudovirales. Its virion is composed of non-enveloped icosahedral head of 60 nm in diameter connected via dodecameric portal with a non-contractile 180 nm long flexible tail. JBD30 tail is terminated with a baseplate embellished with side-tail fibres. Bacteriophage head is built from 415 copies of HK -97 l ike major capsid protein and further decorated on three-fold and quasi-three-fold axis with trimers of minor capsid protein. Data from cryo-electron tomography revealed that bacteriophage JBD30 uses the baseplate side-tail fibers for the attachment to its prey – bacterium Pseudomonas aeruginosa. P. aeruginosa pili type IV, that grow only from bacterial cell pole, serve as a primary receptor for the bacteriophage JBD30. After binding to pili, phage is pulled towards the cell surface by pili retraction, injects its nucleic acid into the bacterium and starts its replication cycle.
ER  -

VALENTOVÁ, Lucie, Tibor FÜZIK a Pavel PLEVKA. Structure of Pseudomonas aeruginosa infecting bacteriophage JBD30 solved by cryo-electron microscopy. In \textit{1st Student Conference in Structural Biology}. 2021.

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