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@proceedings{1826297,
author = {Bíňovský, Ján and Šiborová, Marta and Nováček, Jiří and Van Raaij, M. and Plevka, Pavel},
booktitle = {RUSSIAN INTERNATIONAL CONFERENCE ON CRYO-ELECTRON MICROSCOPY},
doi = {http://dx.doi.org/10.21103/IJBM.11.Suppl_1.OR6},
keywords = {Staphylococcus aureus; bacteriophage; cryo-EM},
language = {eng},
title = {Baseplate Structure of Bacteriophage Phi812 and Mechanism of Cell Wall Binding and Penetration},
url = {https://riccem.org/wp-content/uploads/2021/06/ijbm_11_s1_riccem-2021.pdf},
year = {2021}
}
TY - CONF
ID - 1826297
AU - Bíňovský, Ján - Šiborová, Marta - Nováček, Jiří - Van Raaij, M. - Plevka, Pavel
PY - 2021
TI - Baseplate Structure of Bacteriophage Phi812 and Mechanism of Cell Wall Binding and Penetration
KW - Staphylococcus aureus
KW - bacteriophage
KW - cryo-EM
UR - https://riccem.org/wp-content/uploads/2021/06/ijbm_11_s1_riccem-2021.pdf
N2 - Antibiotic-resistant strains of Staphylococcus aureus cause human infections that are difficult to treat and can lead to death. Bacteriophage (phage) phi812K1/420 from the family Myoviridae infects 95% of clinical isolates of S. aureus and therefore is a promising candidate for a phage therapy agent. As the native phage particle approaches its host cell, phage receptor-binding proteins make a contact with the host cell wall. This interaction triggers a cascade of structural changes in the baseplate resulting in phage tail contraction and genome ejection. Mechanistic description of the baseplate re-organization, however, remains unknown. Methods: Using cryo-electron microscopy (cryo-EM), we studied the baseplate of the phage phi812K1/420. Also, selected proteins involved in the host cell wall binding and penetration were produced in recombinant form and their structures were solved using X-ray crystallography and cryo-EM single-particle reconstruction. Results: We reconstructed the phage baseplate in native and contracted states. The reconstruction of the native baseplate reaches resolution of 4 Å, which enables us to discern individual protein structures. Solved protein structures will be fitted into reconstruction of the contracted baseplate. Conclusion: Our results provide first structural characterization of contractile phage infecting a Gram-positive bacterium. Comparison of the two distinct baseplate states will allow us to describe molecular mechanism of initial stage of phage infection in detail.
ER -
BÍŇOVSKÝ, Ján, Marta ŠIBOROVÁ, Jiří NOVÁČEK, M. VAN RAAIJ a Pavel PLEVKA. Baseplate Structure of Bacteriophage Phi812 and Mechanism of Cell Wall Binding and Penetration. In \textit{RUSSIAN INTERNATIONAL CONFERENCE ON CRYO-ELECTRON MICROSCOPY}. 2021. ISSN~2158-0510. Dostupné z: https://dx.doi.org/10.21103/IJBM.11.Suppl\_{}1.OR6.
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