Delineation of a novel environmental phylogroup of the genus
Acinetobacter encompassing Acinetobacter terrae sp. nov.,
Acinetobacter terrestris sp. nov. and three other tentative
species

J 2021

Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species

NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ, Matěj NEMEC, Petra ŠPANĚLOVÁ et. al.

Základní údaje

Originální název

Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species

Autoři

NEMEC, Alexandr (203 Česká republika), Lenka RADOLFOVÁ-KŘÍŽOVÁ (203 Česká republika), Martina MAIXNEROVÁ (203 Česká republika), Matěj NEMEC (203 Česká republika), Petra ŠPANĚLOVÁ (203 Česká republika), Renata ŠAFRÁNKOVÁ (203 Česká republika), Ondrej ŠEDO (203 Česká republika, garant, domácí), Bruno S. LOPES (826 Velká Británie a Severní Irsko) a Paul G. HIGGINS (276 Německo)

Vydání

Systematic and Applied Microbiology, Urban & Fischer, 2021, 0723-2020

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 4.064

Kód RIV

RIV/00216224:14740/21:00123929

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.syapm.2021.126217

UT WoS

000680042300010

Klíčová slova anglicky

Whole genome sequence; Core genome phylogeny; MALDI-TOF MS; Carbon source assimilation

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:42, Ing. Martina Blahová

Anotace

V originále

This study aimed to define the taxonomic position and structure of a novel, taxonomically unique group of 26 Acinetobacter strains, provisionally designated Taxon 24 (T24). The strains were recovered from soil and freshwater ecosystems (n = 21) or animals (n = 5) in Czechia, Scotland, Germany, the Netherlands and Turkey between 1993 and 2015. They were non-glucose-acidifying, nonhemolytic, nonproteolytic, growing at 32 degrees C and on acetate and ethanol as single carbon sources, but not on 4-hydroxybenzoate and mostly not at 37 degrees C. Their whole-genome sequences were 3.0-3.7 Mb in size, with GC contents of 39.8-41.3%. Based on core genome phylogenetic analysis, the 26 strains formed a distinct Glade within the genus Acinetobacter, with strongly supported subclades termed T24A (n = 11), T24B (n = 8), T24C (n = 2), T24D (n = 3) and T24E (n = 2). The internal genomic ANIb values for these subclades were >94.8%, while the ANIb values between them were <92.5%. The results of MALDI-TOF MS-based analyses agreed with this classification. The five subclades differed from each other in the results of one to six carbon source assimilation tests. Given the genomic and phenotypic distinctness, internal coherence, numbers of available strains and geographically diverse origin of T24A and T24B, we propose the names Acinetobacter terrae sp. nov. and Acinetobacter terrestris sp. nov. for these two taxa, respectively. The type strains are ANC 4282(v) (= CCM 8986(T) = CCUG 73811(T) = CNCTC 8082(T)) and ANC 4471(T) (= CCM 8985(T) = CCUG 73812(T) = CNCTC 8093(T)), respectively. We conclude that these two species together with the other T24 strains represent a widely dispersed Acinetobacter Glade primarily associated with terrestrial ecosystems.

Návaznosti

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ, Matěj NEMEC, Petra ŠPANĚLOVÁ, Renata ŠAFRÁNKOVÁ, Ondrej ŠEDO, Bruno S. LOPES a Paul G. HIGGINS. Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species. Systematic and Applied Microbiology. Urban & Fischer, 2021, roč. 44, č. 4, s. "126217", 9 s. ISSN 0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2021.126217.

@article{1826397,
   author = {Nemec, Alexandr and RadolfováandKřížová, Lenka and Maixnerová, Martina and Nemec, Matěj and Španělová, Petra and Šafránková, Renata and Šedo, Ondrej and Lopes, Bruno S. and Higgins, Paul G.},
   article_number = {4},
   doi = {http://dx.doi.org/10.1016/j.syapm.2021.126217},
   keywords = {Whole genome sequence; Core genome phylogeny; MALDI-TOF MS; Carbon source assimilation},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species},
   url = {https://www.sciencedirect.com/science/article/pii/S0723202021000400?via%3Dihub},
   volume = {44},
   year = {2021}
}

TY  - JOUR
ID  - 1826397
AU  - Nemec, Alexandr - Radolfová-Křížová, Lenka - Maixnerová, Martina - Nemec, Matěj - Španělová, Petra - Šafránková, Renata - Šedo, Ondrej - Lopes, Bruno S. - Higgins, Paul G.
PY  - 2021
TI  - Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species
JF  - Systematic and Applied Microbiology
VL  - 44
IS  - 4
SP  - "126217"
EP  - "126217"
PB  - Urban & Fischer
SN  - 07232020
KW  - Whole genome sequence
KW  - Core genome phylogeny
KW  - MALDI-TOF MS
KW  - Carbon source assimilation
UR  - https://www.sciencedirect.com/science/article/pii/S0723202021000400?via%3Dihub
N2  - This study aimed to define the taxonomic position and structure of a novel, taxonomically unique group of 26 Acinetobacter strains, provisionally designated Taxon 24 (T24). The strains were recovered from soil and freshwater ecosystems (n = 21) or animals (n = 5) in Czechia, Scotland, Germany, the Netherlands and Turkey between 1993 and 2015. They were non-glucose-acidifying, nonhemolytic, nonproteolytic, growing at 32 degrees C and on acetate and ethanol as single carbon sources, but not on 4-hydroxybenzoate and mostly not at 37 degrees C. Their whole-genome sequences were 3.0-3.7 Mb in size, with GC contents of 39.8-41.3%. Based on core genome phylogenetic analysis, the 26 strains formed a distinct Glade within the genus Acinetobacter, with strongly supported subclades termed T24A (n = 11), T24B (n = 8), T24C (n = 2), T24D (n = 3) and T24E (n = 2). The internal genomic ANIb values for these subclades were >94.8%, while the ANIb values between them were <92.5%. The results of MALDI-TOF MS-based analyses agreed with this classification. The five subclades differed from each other in the results of one to six carbon source assimilation tests. Given the genomic and phenotypic distinctness, internal coherence, numbers of available strains and geographically diverse origin of T24A and T24B, we propose the names Acinetobacter terrae sp. nov. and Acinetobacter terrestris sp. nov. for these two taxa, respectively. The type strains are ANC 4282(v) (= CCM 8986(T) = CCUG 73811(T) = CNCTC 8082(T)) and ANC 4471(T) (= CCM 8985(T) = CCUG 73812(T) = CNCTC 8093(T)), respectively. We conclude that these two species together with the other T24 strains represent a widely dispersed Acinetobacter Glade primarily associated with terrestrial ecosystems.
ER  -

NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ, Matěj NEMEC, Petra ŠPANĚLOVÁ, Renata ŠAFRÁNKOVÁ, Ondrej ŠEDO, Bruno S. LOPES a Paul G. HIGGINS. Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species. \textit{Systematic and Applied Microbiology}. Urban \&{} Fischer, 2021, roč.~44, č.~4, s.~''126217'', 9 s. ISSN~0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2021.126217.

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