Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following
Heat Stress where They Regulate the Expression of a Similar Set
of Genes

J 2021

Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes

WIESE, Anna J., Lenka STEINBACHOVÁ, Ljudmilla TIMOFEJEVA, Vojtěch ČERMÁK, Božena KLODOVÁ et. al.

Základní údaje

Originální název

Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes

Autoři

WIESE, Anna J. (203 Česká republika), Lenka STEINBACHOVÁ (203 Česká republika), Ljudmilla TIMOFEJEVA (203 Česká republika), Vojtěch ČERMÁK (203 Česká republika), Božena KLODOVÁ (203 Česká republika), Sri Ranjani GANJI (203 Česká republika, domácí), Mariana LIMONES-MENDEZ (203 Česká republika), Pavel BOKVAJ (203 Česká republika), Said HAFIDH (203 Česká republika), David POTĚŠIL (203 Česká republika, garant, domácí) a David HONYS (203 Česká republika)

Vydání

International Journal of Molecular Sciences, Molecular Diversity Preservation International, 2021, 1661-6596

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 2.598 v roce 2011

Kód RIV

RIV/00216224:14740/21:00123930

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/ijms22020530

UT WoS

000611888100001

Klíčová slova anglicky

bZIP; heat stress; Arabidopsis; 14– 3– 3; localization; transcriptomics

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 10. 2024 14:44, Ing. Martina Blahová

Anotace

V originále

Heat stress (HS) is a major abiotic stress that negatively impacts crop yields across the globe. Plants respond to elevated temperatures by changing gene expression, mediated by transcription factors (TFs) functioning to enhance HS tolerance. The involvement of Group I bZIP TFs in the heat stress response (HSR) is not known. In this study, bZIP18 and bZIP52 were investigated for their possible role in the HSR. Localization experiments revealed their nuclear accumulation following heat stress, which was found to be triggered by dephosphorylation. Both TFs were found to possess two motifs containing serine residues that are candidates for phosphorylation. These motifs are recognized by 14-3-3 proteins, and bZIP18 and bZIP52 were found to bind 14-3-3 epsilon, the interaction of which sequesters them to the cytoplasm. Mutation of both residues abolished 14-3-3 epsilon interaction and led to a strict nuclear localization for both TFs. RNA-seq analysis revealed coordinated downregulation of several metabolic pathways including energy metabolism and translation, and upregulation of numerous lncRNAs in particular. These results support the idea that bZIP18 and bZIP52 are sequestered to the cytoplasm under control conditions, and that heat stress leads to their re-localization to nuclei, where they jointly regulate gene expression.

Návaznosti

EF16_019/0000738, projekt VaV

Název: Centrum experimentální biologie rostlin

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LM2018129, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

WIESE, Anna J., Lenka STEINBACHOVÁ, Ljudmilla TIMOFEJEVA, Vojtěch ČERMÁK, Božena KLODOVÁ, Sri Ranjani GANJI, Mariana LIMONES-MENDEZ, Pavel BOKVAJ, Said HAFIDH, David POTĚŠIL a David HONYS. Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes. International Journal of Molecular Sciences. Molecular Diversity Preservation International, 2021, roč. 22, č. 2, s. "530", 24 s. ISSN 1661-6596. Dostupné z: https://dx.doi.org/10.3390/ijms22020530.

@article{1826439,
   author = {Wiese, Anna J. and Steinbachová, Lenka and Timofejeva, Ljudmilla and Čermák, Vojtěch and Klodová, Božena and Ganji, Sri Ranjani and LimonesandMendez, Mariana and Bokvaj, Pavel and Hafidh, Said and Potěšil, David and Honys, David},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/ijms22020530},
   keywords = {bZIP; heat stress; Arabidopsis; 14– 3– 3; localization; transcriptomics},
   language = {eng},
   issn = {1661-6596},
   journal = {International Journal of Molecular Sciences},
   title = {Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes},
   url = {https://www.mdpi.com/1422-0067/22/2/530},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1826439
AU  - Wiese, Anna J. - Steinbachová, Lenka - Timofejeva, Ljudmilla - Čermák, Vojtěch - Klodová, Božena - Ganji, Sri Ranjani - Limones-Mendez, Mariana - Bokvaj, Pavel - Hafidh, Said - Potěšil, David - Honys, David
PY  - 2021
TI  - Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 2
SP  - "530"
EP  - "530"
PB  - Molecular Diversity Preservation International
SN  - 16616596
KW  - bZIP
KW  - heat stress
KW  - Arabidopsis
KW  - 14–
KW  - 3–
KW  - 3
KW  - localization
KW  - transcriptomics
UR  - https://www.mdpi.com/1422-0067/22/2/530
N2  - Heat stress (HS) is a major abiotic stress that negatively impacts crop yields across the globe. Plants respond to elevated temperatures by changing gene expression, mediated by transcription factors (TFs) functioning to enhance HS tolerance. The involvement of Group I bZIP TFs in the heat stress response (HSR) is not known. In this study, bZIP18 and bZIP52 were investigated for their possible role in the HSR. Localization experiments revealed their nuclear accumulation following heat stress, which was found to be triggered by dephosphorylation. Both TFs were found to possess two motifs containing serine residues that are candidates for phosphorylation. These motifs are recognized by 14-3-3 proteins, and bZIP18 and bZIP52 were found to bind 14-3-3 epsilon, the interaction of which sequesters them to the cytoplasm. Mutation of both residues abolished 14-3-3 epsilon interaction and led to a strict nuclear localization for both TFs. RNA-seq analysis revealed coordinated downregulation of several metabolic pathways including energy metabolism and translation, and upregulation of numerous lncRNAs in particular. These results support the idea that bZIP18 and bZIP52 are sequestered to the cytoplasm under control conditions, and that heat stress leads to their re-localization to nuclei, where they jointly regulate gene expression.
ER  -

WIESE, Anna J., Lenka STEINBACHOVÁ, Ljudmilla TIMOFEJEVA, Vojtěch ČERMÁK, Božena KLODOVÁ, Sri Ranjani GANJI, Mariana LIMONES-MENDEZ, Pavel BOKVAJ, Said HAFIDH, David POTĚŠIL a David HONYS. Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes. \textit{International Journal of Molecular Sciences}. Molecular Diversity Preservation International, 2021, roč.~22, č.~2, s.~''530'', 24 s. ISSN~1661-6596. Dostupné z: https://dx.doi.org/10.3390/ijms22020530.

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