| WIESE, Anna J., Lenka STEINBACHOVÁ, Ljudmilla TIMOFEJEVA, Vojtěch ČERMÁK, Božena KLODOVÁ, Sri Ranjani GANJI, Mariana LIMONES-MENDEZ, Pavel BOKVAJ, Said HAFIDH, David POTĚŠIL and David HONYS. Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes. International Journal of Molecular Sciences. Molecular Diversity Preservation International, 2021, vol. 22, No 2, p. "530", 24 pp. ISSN 1661-6596. Available from: https://dx.doi.org/10.3390/ijms22020530. |
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@article{1826439,
author = {Wiese, Anna J. and Steinbachová, Lenka and Timofejeva, Ljudmilla and Čermák, Vojtěch and Klodová, Božena and Ganji, Sri Ranjani and LimonesandMendez, Mariana and Bokvaj, Pavel and Hafidh, Said and Potěšil, David and Honys, David},
article_number = {2},
doi = {http://dx.doi.org/10.3390/ijms22020530},
keywords = {bZIP; heat stress; Arabidopsis; 14– 3– 3; localization; transcriptomics},
language = {eng},
issn = {1661-6596},
journal = {International Journal of Molecular Sciences},
title = {Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes},
url = {https://www.mdpi.com/1422-0067/22/2/530},
volume = {22},
year = {2021}
}
TY - JOUR
ID - 1826439
AU - Wiese, Anna J. - Steinbachová, Lenka - Timofejeva, Ljudmilla - Čermák, Vojtěch - Klodová, Božena - Ganji, Sri Ranjani - Limones-Mendez, Mariana - Bokvaj, Pavel - Hafidh, Said - Potěšil, David - Honys, David
PY - 2021
TI - Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes
JF - International Journal of Molecular Sciences
VL - 22
IS - 2
SP - "530"
EP - "530"
PB - Molecular Diversity Preservation International
SN - 16616596
KW - bZIP
KW - heat stress
KW - Arabidopsis
KW - 14–
KW - 3–
KW - 3
KW - localization
KW - transcriptomics
UR - https://www.mdpi.com/1422-0067/22/2/530
N2 - Heat stress (HS) is a major abiotic stress that negatively impacts crop yields across the globe. Plants respond to elevated temperatures by changing gene expression, mediated by transcription factors (TFs) functioning to enhance HS tolerance. The involvement of Group I bZIP TFs in the heat stress response (HSR) is not known. In this study, bZIP18 and bZIP52 were investigated for their possible role in the HSR. Localization experiments revealed their nuclear accumulation following heat stress, which was found to be triggered by dephosphorylation. Both TFs were found to possess two motifs containing serine residues that are candidates for phosphorylation. These motifs are recognized by 14-3-3 proteins, and bZIP18 and bZIP52 were found to bind 14-3-3 epsilon, the interaction of which sequesters them to the cytoplasm. Mutation of both residues abolished 14-3-3 epsilon interaction and led to a strict nuclear localization for both TFs. RNA-seq analysis revealed coordinated downregulation of several metabolic pathways including energy metabolism and translation, and upregulation of numerous lncRNAs in particular. These results support the idea that bZIP18 and bZIP52 are sequestered to the cytoplasm under control conditions, and that heat stress leads to their re-localization to nuclei, where they jointly regulate gene expression.
ER -
WIESE, Anna J., Lenka STEINBACHOVÁ, Ljudmilla TIMOFEJEVA, Vojtěch ČERMÁK, Božena KLODOVÁ, Sri Ranjani GANJI, Mariana LIMONES-MENDEZ, Pavel BOKVAJ, Said HAFIDH, David POTĚŠIL and David HONYS. Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes. \textit{International Journal of Molecular Sciences}. Molecular Diversity Preservation International, 2021, vol.~22, No~2, p.~''530'', 24 pp. ISSN~1661-6596. Available from: https://dx.doi.org/10.3390/ijms22020530.
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