Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation

BÍŇOVSKÝ, Ján, Marta ŠIBOROVÁ, Jiří NOVÁČEK, M. VAN RAAIJ a Pavel PLEVKA. Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation. In Phage/Virus Assembly 2021. 2021.

Základní údaje

• Originální název: Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation
• Autoři: BÍŇOVSKÝ, Ján (703 Slovensko, domácí), Marta ŠIBOROVÁ (203 Česká republika, domácí), Jiří NOVÁČEK (203 Česká republika, domácí), M. VAN RAAIJ a Pavel PLEVKA (203 Česká republika, garant, domácí).
• Vydání: Phage/Virus Assembly 2021, 2021.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 10607 Virology
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Kód RIV: RIV/00216224:14740/21:00123931
• Organizační jednotka: Středoevropský technologický institut
• Klíčová slova anglicky: Staphylococcus aureus; Bacteriophage; Myoviridae
• Štítky: rivok

Anotace

Antibiotic-resistant strains of Staphylococcus aureus cause human infections that are difficult to treat and can lead to death . Bacteriophage (phage) phi812K1/420 from the family Myoviridae infects 95% of S. aureus isolates and therefore is a promising candidate for a phage therapy agent . As the native phage particle approaches its host cell, phage receptor-binding proteins make a contact with the host cell wall. This interaction triggers a cascade of structural changes in the baseplate, resulting in phage tail contraction and genome ejection . Mechanistic description of the baseplate re-organization, however, remains unknown. Using cryo-electron microscopy (cryo-EM), we reconstructed the phage baseplate in native and contracted states. The reconstruction of the center of native baseplate reaches resolution of 4 Å, which enables us to build individual protein structures. Also, selected proteins involved in host cell wall binding and penetration were produced in recombinant form and their structures were solved using X-ray crystallography and cryo-EM single-particle reconstruction. The protein structures will be fitted into reconstruction of the contracted baseplate. Our results provide first structural characterisation of contractile phage infecting a Gram-positive bacterium. Comparison of the two distinct baseplate states will allow us to describe molecular mechanism of initial stage of phage infection in detail.

Návaznosti

• LL1906, projekt VaV: Název: Replikace fágů v bakteriálním biofilmu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Replikace fágů v bakteriálním biofilmu