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@proceedings{1826480, author = {Bíňovský, Ján and Šiborová, Marta and Nováček, Jiří and Van Raaij, M. and Plevka, Pavel}, booktitle = {Phage/Virus Assembly 2021}, keywords = {Staphylococcus aureus; Bacteriophage; Myoviridae}, language = {eng}, title = {Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation}, url = {https://pvaconference.wixsite.com/pva2021}, year = {2021} }
TY - CONF ID - 1826480 AU - Bíňovský, Ján - Šiborová, Marta - Nováček, Jiří - Van Raaij, M. - Plevka, Pavel PY - 2021 TI - Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation KW - Staphylococcus aureus KW - Bacteriophage KW - Myoviridae UR - https://pvaconference.wixsite.com/pva2021 N2 - Antibiotic-resistant strains of Staphylococcus aureus cause human infections that are difficult to treat and can lead to death . Bacteriophage (phage) phi812K1/420 from the family Myoviridae infects 95% of S. aureus isolates and therefore is a promising candidate for a phage therapy agent . As the native phage particle approaches its host cell, phage receptor-binding proteins make a contact with the host cell wall. This interaction triggers a cascade of structural changes in the baseplate, resulting in phage tail contraction and genome ejection . Mechanistic description of the baseplate re-organization, however, remains unknown. Using cryo-electron microscopy (cryo-EM), we reconstructed the phage baseplate in native and contracted states. The reconstruction of the center of native baseplate reaches resolution of 4 Å, which enables us to build individual protein structures. Also, selected proteins involved in host cell wall binding and penetration were produced in recombinant form and their structures were solved using X-ray crystallography and cryo-EM single-particle reconstruction. The protein structures will be fitted into reconstruction of the contracted baseplate. Our results provide first structural characterisation of contractile phage infecting a Gram-positive bacterium. Comparison of the two distinct baseplate states will allow us to describe molecular mechanism of initial stage of phage infection in detail. ER -
BÍŇOVSKÝ, Ján, Marta ŠIBOROVÁ, Jiří NOVÁČEK, M. VAN RAAIJ a Pavel PLEVKA. Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation. In \textit{Phage/Virus Assembly 2021}. 2021.
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