Conformational changes of short tail fibers enable genome
delivery of Podoviridae phage SU10

k 2021

Conformational changes of short tail fibers enable genome delivery of Podoviridae phage SU10

ŠIBOROVÁ, Marta, Tibor FÜZIK, Michaela PROCHÁZKOVÁ, Jiří NOVÁČEK, Martin BENEŠÍK et. al.

Základní údaje

Originální název

Conformational changes of short tail fibers enable genome delivery of Podoviridae phage SU10

Autoři

ŠIBOROVÁ, Marta (203 Česká republika, domácí), Tibor FÜZIK (703 Slovensko, domácí), Michaela PROCHÁZKOVÁ (203 Česká republika, domácí), Jiří NOVÁČEK (203 Česká republika, domácí), Martin BENEŠÍK (203 Česká republika, domácí), AS. NILSSON a Pavel PLEVKA (203 Česká republika, garant, domácí)

Vydání

Phage/Virus Assembly 2021, 2021

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10607 Virology

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/21:00123936

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

phage SU10; Kuravirus; cryo-electron tomography

Štítky

rivok

Příznaky

Mezinárodní význam

Změněno: 24. 1. 2022 16:04, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Escherichia coli phage SU10 belongs to the genus Kuravirusfrom the family Podoviridaeof phages with short tails. Kuraviruseshave the potential to be used in phage therapy against antibiotic-resistant bacterial infections, however, their structure and genome delivery mechanism have not been characterized yet. Here we used cryo-electron microscopy of purified SU10 particles and cryo-electron tomography of infected E. colicells to describe the structural changes of the phage tail that are required for its genome ejection and delivery. The binding of the long tail fibers to the receptors in the outer bacterial membrane is followed by the straightening of nozzle proteins and rotation of short tail fibers by 135°. In the new arrangement, the nozzle proteins and short tail fibers alternate to form a nozzle that prolongs the tail by 28 nm. To open the tail channel, the tail needle detaches from the nozzle proteins. The inner core proteins of five types, one of which has the predicted peptidoglycan-degradation activity, are ejected from the SU10 head before or together with the genome. The nozzle with the putative extension formed by the inner core proteins enables the delivery of the SU10 genome into the bacterial cytoplasm. Tails of podoviridae phages are defined as short and non-contractile, however, here we show that upon infection kuravirus tails extend into a nozzle for genome delivery.

Návaznosti

LL1906, projekt VaV

Název: Replikace fágů v bakteriálním biofilmu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Replikace fágů v bakteriálním biofilmu

Citovat

ŠIBOROVÁ, Marta, Tibor FÜZIK, Michaela PROCHÁZKOVÁ, Jiří NOVÁČEK, Martin BENEŠÍK, AS. NILSSON a Pavel PLEVKA. Conformational changes of short tail fibers enable genome delivery of Podoviridae phage SU10. In Phage/Virus Assembly 2021. 2021.

@proceedings{1826506,
   author = {Šiborová, Marta and Füzik, Tibor and Procházková, Michaela and Nováček, Jiří and Benešík, Martin and Nilsson, AS. and Plevka, Pavel},
   booktitle = {Phage/Virus Assembly 2021},
   keywords = {phage SU10; Kuravirus; cryo-electron tomography},
   language = {eng},
   title = {Conformational changes of short tail fibers enable genome delivery of Podoviridae phage SU10},
   url = {https://pvaconference.wixsite.com/pva2021},
   year = {2021}
}

TY  - CONF
ID  - 1826506
AU  - Šiborová, Marta - Füzik, Tibor - Procházková, Michaela - Nováček, Jiří - Benešík, Martin - Nilsson, AS. - Plevka, Pavel
PY  - 2021
TI  - Conformational changes of short tail fibers enable genome delivery of Podoviridae phage SU10
KW  - phage SU10
KW  - Kuravirus
KW  - cryo-electron tomography
UR  - https://pvaconference.wixsite.com/pva2021
N2  - Escherichia coli phage SU10 belongs to the genus Kuravirusfrom the family Podoviridaeof phages with short tails. Kuraviruseshave the potential to be used in phage therapy against antibiotic-resistant bacterial infections, however, their structure and genome delivery mechanism have not been characterized yet. Here we used cryo-electron microscopy of purified SU10 particles and cryo-electron tomography of infected E. colicells to describe the structural changes of the phage tail that are required for its genome ejection and delivery. The binding of the long tail fibers to the receptors in the outer bacterial membrane is followed by the straightening of nozzle proteins and rotation of short tail fibers by 135°. In the new arrangement, the nozzle proteins and short tail fibers alternate to form a nozzle that prolongs the tail by 28 nm. To open the tail channel, the tail needle detaches from the nozzle proteins. The inner core proteins of five types, one of which has the predicted peptidoglycan-degradation activity, are ejected from the SU10 head before or together with the genome. The nozzle with the putative extension formed by the inner core proteins enables the delivery of the SU10 genome into the bacterial cytoplasm. Tails of podoviridae phages are defined as short and non-contractile, however, here we show that upon infection kuravirus tails extend into a nozzle for genome delivery.
ER  -

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