ŠTĚPÁNOVÁ, Hana, Karolina HLAVOVÁ, Radek MACHÁT, E JEKLOVA and Martin FALDYNA. The effect of polyclonal activators on rabbit B cell antibody production. RESEARCH IN VETERINARY SCIENCE. OXFORD: ELSEVIER SCI LTD, 2020, vol. 130, p. 193-196. ISSN 0034-5288. Available from: https://dx.doi.org/10.1016/j.rvsc.2020.03.022.
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Basic information
Original name The effect of polyclonal activators on rabbit B cell antibody production
Authors ŠTĚPÁNOVÁ, Hana, Karolina HLAVOVÁ, Radek MACHÁT, E JEKLOVA and Martin FALDYNA.
Edition RESEARCH IN VETERINARY SCIENCE, OXFORD, ELSEVIER SCI LTD, 2020, 0034-5288.
Other information
Original language English
Type of outcome Article in a journal
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.534
Doi http://dx.doi.org/10.1016/j.rvsc.2020.03.022
UT WoS 000525471600028
Tags RIV ne
Changed by Changed by: Mgr. Bc. Radek Machát, Ph.D., učo 270568. Changed: 27/1/2022 10:54.
Abstract
Stimulation with polyclonal activators is a tool to increase antibody secretion in B cells. The aim of the present study was to select the most effective common commercially available polyclonal activators of rabbit B cells. Specifically, type B oligodeoxynucleotides with unmethylated deoxycytidyl-deoxyguanosine dinucleotides (CpG-ODN), recombinant rabbit interleukin-2 (rrIL-2), lipopolysaccharide (LPS), pokeweed mitogen (PWM) and Resiquimod (R848) were tested on B cells isolated from blood and spleen by fluorescence-activated cell sorting. Based on the obtained data, stimulation with CpG-ODN induced the highest antigen-specific antibody levels detected by ELISA in supernatants when a single activator was used. In contrast, LPS, PWM and R848 showed a weak or no stimulatory effect. Stimulation with a mix of activators was more effective than CpG-ODN alone, which indicates a synergistic effect in the stimulation of antibody production.
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