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@article{1827859, author = {Štěpánová, Hana and Hlavová, Karolina and Machát, Radek and Jeklova, E and Faldyna, Martin}, article_location = {OXFORD}, doi = {http://dx.doi.org/10.1016/j.rvsc.2020.03.022}, language = {eng}, issn = {0034-5288}, journal = {RESEARCH IN VETERINARY SCIENCE}, title = {The effect of polyclonal activators on rabbit B cell antibody production}, volume = {130}, year = {2020} }
TY - JOUR ID - 1827859 AU - Štěpánová, Hana - Hlavová, Karolina - Machát, Radek - Jeklova, E - Faldyna, Martin PY - 2020 TI - The effect of polyclonal activators on rabbit B cell antibody production JF - RESEARCH IN VETERINARY SCIENCE VL - 130 SP - 193-196 EP - 193-196 PB - ELSEVIER SCI LTD SN - 00345288 N2 - Stimulation with polyclonal activators is a tool to increase antibody secretion in B cells. The aim of the present study was to select the most effective common commercially available polyclonal activators of rabbit B cells. Specifically, type B oligodeoxynucleotides with unmethylated deoxycytidyl-deoxyguanosine dinucleotides (CpG-ODN), recombinant rabbit interleukin-2 (rrIL-2), lipopolysaccharide (LPS), pokeweed mitogen (PWM) and Resiquimod (R848) were tested on B cells isolated from blood and spleen by fluorescence-activated cell sorting. Based on the obtained data, stimulation with CpG-ODN induced the highest antigen-specific antibody levels detected by ELISA in supernatants when a single activator was used. In contrast, LPS, PWM and R848 showed a weak or no stimulatory effect. Stimulation with a mix of activators was more effective than CpG-ODN alone, which indicates a synergistic effect in the stimulation of antibody production. ER -
ŠTĚPÁNOVÁ, Hana, Karolina HLAVOVÁ, Radek MACHÁT, E JEKLOVA and Martin FALDYNA. The effect of polyclonal activators on rabbit B cell antibody production. \textit{RESEARCH IN VETERINARY SCIENCE}. OXFORD: ELSEVIER SCI LTD, 2020, vol.~130, p.~193-196. ISSN~0034-5288. Available from: https://dx.doi.org/10.1016/j.rvsc.2020.03.022.
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