Multiscale Analysis of Extracellular Matrix Remodeling in the
Failing Heart

J 2021

Multiscale Analysis of Extracellular Matrix Remodeling in the Failing Heart

PERESTRELO, A. R., A. C. SILVA, J. OLIVER-DE LA CRUZ, Fabiana MARTINO, Vladimir HORVATH et. al.

Základní údaje

Originální název

Multiscale Analysis of Extracellular Matrix Remodeling in the Failing Heart

Autoři

PERESTRELO, A. R. (garant), A. C. SILVA, J. OLIVER-DE LA CRUZ, Fabiana MARTINO (380 Itálie, domácí), Vladimir HORVATH (203 Česká republika), Guido CALUORI (380 Itálie, domácí), Ondrej POLANSKY (203 Česká republika), Vladimir VINARSKY (203 Česká republika), G. AZZATO, G. DE MARCO, Víta ŽAMPACHOVÁ (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí), S. PAGLIARI, A. RAINER, P. PINTO-DO-O, A. CARAVELLA, Kamila KOCI (203 Česká republika), D. S. NASCIMENTO a Giancarlo FORTE (380 Itálie)

Vydání

Circulation research, Dallas, American Heart Association, 2021, 0009-7330

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 23.213

Kód RIV

RIV/00216224:14110/21:00123975

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1161/CIRCRESAHA.120.317685

UT WoS

000639316500005

Klíčová slova anglicky

cardiomyopathy; dilated; elasticity; extracellular matrix; fibroblasts

Štítky

14110112, 14110513, CF BIOIT, CF GEN, CF NANO

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 5. 2022 12:59, Mgr. Tereza Miškechová

Anotace

V originále

Rationale: Cardiac ECM (extracellular matrix) comprises a dynamic molecular network providing structural support to heart tissue function. Understanding the impact of ECM remodeling on cardiac cells during heart failure (HF) is essential to prevent adverse ventricular remodeling and restore organ functionality in affected patients. Objectives: We aimed to (1) identify consistent modifications to cardiac ECM structure and mechanics that contribute to HF and (2) determine the underlying molecular mechanisms. Methods and Results: We first performed decellularization of human and murine ECM (decellularized ECM) and then analyzed the pathological changes occurring in decellularized ECM during HF by atomic force microscopy, 2-photon microscopy, high-resolution 3-dimensional image analysis, and computational fluid dynamics simulation. We then performed molecular and functional assays in patient-derived cardiac fibroblasts based on YAP (yes-associated protein)-transcriptional enhanced associate domain (TEAD) mechanosensing activity and collagen contraction assays. The analysis of HF decellularized ECM resulting from ischemic or dilated cardiomyopathy, as well as from mouse infarcted tissue, identified a common pattern of modifications in their 3-dimensional topography. As compared with healthy heart, HF ECM exhibited aligned, flat, and compact fiber bundles, with reduced elasticity and organizational complexity. At the molecular level, RNA sequencing of HF cardiac fibroblasts highlighted the overrepresentation of dysregulated genes involved in ECM organization, or being connected to TGF beta 1 (transforming growth factor beta 1), interleukin-1, TNF-alpha, and BDNF signaling pathways. Functional tests performed on HF cardiac fibroblasts pointed at mechanosensor YAP as a key player in ECM remodeling in the diseased heart via transcriptional activation of focal adhesion assembly. Finally, in vitro experiments clarified pathological cardiac ECM prevents cell homing, thus providing further hints to identify a possible window of action for cell therapy in cardiac diseases. Conclusions: Our multiparametric approach has highlighted repercussions of ECM remodeling on cell homing, cardiac fibroblast activation, and focal adhesion protein expression via hyperactivated YAP signaling during HF.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

Citovat

PERESTRELO, A. R., A. C. SILVA, J. OLIVER-DE LA CRUZ, Fabiana MARTINO, Vladimir HORVATH, Guido CALUORI, Ondrej POLANSKY, Vladimir VINARSKY, G. AZZATO, G. DE MARCO, Víta ŽAMPACHOVÁ, Petr SKLÁDAL, S. PAGLIARI, A. RAINER, P. PINTO-DO-O, A. CARAVELLA, Kamila KOCI, D. S. NASCIMENTO a Giancarlo FORTE. Multiscale Analysis of Extracellular Matrix Remodeling in the Failing Heart. Circulation research. Dallas: American Heart Association, 2021, roč. 128, č. 1, s. 24-38. ISSN 0009-7330. Dostupné z: https://dx.doi.org/10.1161/CIRCRESAHA.120.317685.

@article{1827937,
   author = {Perestrelo, A. R. and Silva, A. C. and OliverandDe La Cruz, J. and Martino, Fabiana and Horvath, Vladimir and Caluori, Guido and Polansky, Ondrej and Vinarsky, Vladimir and Azzato, G. and de Marco, G. and Žampachová, Víta and Skládal, Petr and Pagliari, S. and Rainer, A. and PintoanddoandO, P. and Caravella, A. and Koci, Kamila and Nascimento, D. S. and Forte, Giancarlo},
   article_location = {Dallas},
   article_number = {1},
   doi = {http://dx.doi.org/10.1161/CIRCRESAHA.120.317685},
   keywords = {cardiomyopathy; dilated; elasticity; extracellular matrix; fibroblasts},
   language = {eng},
   issn = {0009-7330},
   journal = {Circulation research},
   title = {Multiscale Analysis of Extracellular Matrix Remodeling in the Failing Heart},
   url = {https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.120.317685},
   volume = {128},
   year = {2021}
}

TY  - JOUR
ID  - 1827937
AU  - Perestrelo, A. R. - Silva, A. C. - Oliver-De La Cruz, J. - Martino, Fabiana - Horvath, Vladimir - Caluori, Guido - Polansky, Ondrej - Vinarsky, Vladimir - Azzato, G. - de Marco, G. - Žampachová, Víta - Skládal, Petr - Pagliari, S. - Rainer, A. - Pinto-do-O, P. - Caravella, A. - Koci, Kamila - Nascimento, D. S. - Forte, Giancarlo
PY  - 2021
TI  - Multiscale Analysis of Extracellular Matrix Remodeling in the Failing Heart
JF  - Circulation research
VL  - 128
IS  - 1
SP  - 24-38
EP  - 24-38
PB  - American Heart Association
SN  - 00097330
KW  - cardiomyopathy
KW  - dilated
KW  - elasticity
KW  - extracellular matrix
KW  - fibroblasts
UR  - https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.120.317685
N2  - Rationale: Cardiac ECM (extracellular matrix) comprises a dynamic molecular network providing structural support to heart tissue function. Understanding the impact of ECM remodeling on cardiac cells during heart failure (HF) is essential to prevent adverse ventricular remodeling and restore organ functionality in affected patients. Objectives: We aimed to (1) identify consistent modifications to cardiac ECM structure and mechanics that contribute to HF and (2) determine the underlying molecular mechanisms. Methods and Results: We first performed decellularization of human and murine ECM (decellularized ECM) and then analyzed the pathological changes occurring in decellularized ECM during HF by atomic force microscopy, 2-photon microscopy, high-resolution 3-dimensional image analysis, and computational fluid dynamics simulation. We then performed molecular and functional assays in patient-derived cardiac fibroblasts based on YAP (yes-associated protein)-transcriptional enhanced associate domain (TEAD) mechanosensing activity and collagen contraction assays. The analysis of HF decellularized ECM resulting from ischemic or dilated cardiomyopathy, as well as from mouse infarcted tissue, identified a common pattern of modifications in their 3-dimensional topography. As compared with healthy heart, HF ECM exhibited aligned, flat, and compact fiber bundles, with reduced elasticity and organizational complexity. At the molecular level, RNA sequencing of HF cardiac fibroblasts highlighted the overrepresentation of dysregulated genes involved in ECM organization, or being connected to TGF beta 1 (transforming growth factor beta 1), interleukin-1, TNF-alpha, and BDNF signaling pathways. Functional tests performed on HF cardiac fibroblasts pointed at mechanosensor YAP as a key player in ECM remodeling in the diseased heart via transcriptional activation of focal adhesion assembly. Finally, in vitro experiments clarified pathological cardiac ECM prevents cell homing, thus providing further hints to identify a possible window of action for cell therapy in cardiac diseases. Conclusions: Our multiparametric approach has highlighted repercussions of ECM remodeling on cell homing, cardiac fibroblast activation, and focal adhesion protein expression via hyperactivated YAP signaling during HF.
ER  -

PERESTRELO, A. R., A. C. SILVA, J. OLIVER-DE LA CRUZ, Fabiana MARTINO, Vladimir HORVATH, Guido CALUORI, Ondrej POLANSKY, Vladimir VINARSKY, G. AZZATO, G. DE MARCO, Víta ŽAMPACHOVÁ, Petr SKLÁDAL, S. PAGLIARI, A. RAINER, P. PINTO-DO-O, A. CARAVELLA, Kamila KOCI, D. S. NASCIMENTO a Giancarlo FORTE. Multiscale Analysis of Extracellular Matrix Remodeling in the Failing Heart. \textit{Circulation research}. Dallas: American Heart Association, 2021, roč.~128, č.~1, s.~24-38. ISSN~0009-7330. Dostupné z: https://dx.doi.org/10.1161/CIRCRESAHA.120.317685.

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