Visualization of hydrogen atoms in a perdeuterated
lectin-fucose complex reveals key details of
protein-carbohydrate interactions

J 2021

Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions

GAJDOŠ, Lukáš, Matthew P. BLAKELEY, Atul KUMAR, Michaela WIMMEROVÁ, Michaela HAERTLEIN et. al.

Základní údaje

Originální název

Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions

Autoři

GAJDOŠ, Lukáš (703 Slovensko), Matthew P. BLAKELEY (250 Francie), Atul KUMAR (356 Indie, domácí), Michaela WIMMEROVÁ (203 Česká republika, garant, domácí), Michaela HAERTLEIN (250 Francie), Trevor V. FORSYTH (250 Francie), Anne IMBERTY (250 Francie) a Juliette M. DEVOS (250 Francie)

Vydání

Structure, CAMBRIDGE, CELL PRESS, 2021, 0969-2126

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.871

Kód RIV

RIV/00216224:14310/21:00119632

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.str.2021.03.003

UT WoS

000692378500008

Klíčová slova anglicky

lectin; neutron diffraction; X-ray

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2022 10:57, Mgr. Marie Šípková, DiS.

Anotace

V originále

Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the roomtemperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-pi stacking interactions between fucose and the aromatic amino acids in the binding site.

Návaznosti

GA18-18964S, projekt VaV

Název: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Grantová agentura ČR, Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Citovat

GAJDOŠ, Lukáš, Matthew P. BLAKELEY, Atul KUMAR, Michaela WIMMEROVÁ, Michaela HAERTLEIN, Trevor V. FORSYTH, Anne IMBERTY a Juliette M. DEVOS. Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. Structure. CAMBRIDGE: CELL PRESS, 2021, roč. 29, č. 9, s. "1003"-"1013.e4", 15 s. ISSN 0969-2126. Dostupné z: https://dx.doi.org/10.1016/j.str.2021.03.003.

@article{1830859,
   author = {Gajdoš, Lukáš and Blakeley, Matthew P. and Kumar, Atul and Wimmerová, Michaela and Haertlein, Michaela and Forsyth, Trevor V. and Imberty, Anne and Devos, Juliette M.},
   article_location = {CAMBRIDGE},
   article_number = {9},
   doi = {http://dx.doi.org/10.1016/j.str.2021.03.003},
   keywords = {lectin; neutron diffraction; X-ray},
   language = {eng},
   issn = {0969-2126},
   journal = {Structure},
   title = {Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions},
   url = {https://www.sciencedirect.com/science/article/pii/S0969212621000769?via%3Dihub},
   volume = {29},
   year = {2021}
}

TY  - JOUR
ID  - 1830859
AU  - Gajdoš, Lukáš - Blakeley, Matthew P. - Kumar, Atul - Wimmerová, Michaela - Haertlein, Michaela - Forsyth, Trevor V. - Imberty, Anne - Devos, Juliette M.
PY  - 2021
TI  - Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions
JF  - Structure
VL  - 29
IS  - 9
SP  - "1003"-"1013.e4"
EP  - "1003"-"1013.e4"
PB  - CELL PRESS
SN  - 09692126
KW  - lectin
KW  - neutron diffraction
KW  - X-ray
UR  - https://www.sciencedirect.com/science/article/pii/S0969212621000769?via%3Dihub
N2  - Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the roomtemperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-pi stacking interactions between fucose and the aromatic amino acids in the binding site.
ER  -

GAJDOŠ, Lukáš, Matthew P. BLAKELEY, Atul KUMAR, Michaela WIMMEROVÁ, Michaela HAERTLEIN, Trevor V. FORSYTH, Anne IMBERTY a Juliette M. DEVOS. Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. \textit{Structure}. CAMBRIDGE: CELL PRESS, 2021, roč.~29, č.~9, s.~''1003''-''1013.e4'', 15 s. ISSN~0969-2126. Dostupné z: https://dx.doi.org/10.1016/j.str.2021.03.003.

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