Virion Structure and In Vitro Genome Release Mechanism of
Dicistrovirus Kashmir Bee Virus

J 2021

Virion Structure and In Vitro Genome Release Mechanism of Dicistrovirus Kashmir Bee Virus

MUKHAMEDOVA, Liya, Tibor FÜZIK, Jiří NOVÁČEK, Dominik HREBÍK, A. PRIDAL et. al.

Základní údaje

Originální název

Virion Structure and In Vitro Genome Release Mechanism of Dicistrovirus Kashmir Bee Virus

Autoři

MUKHAMEDOVA, Liya (643 Rusko, domácí), Tibor FÜZIK (703 Slovensko, domácí), Jiří NOVÁČEK (203 Česká republika, domácí), Dominik HREBÍK (703 Slovensko, domácí), A. PRIDAL, G.A. MARTI, D.M.A. GUERIN a Pavel PLEVKA (203 Česká republika, garant, domácí)

Vydání

Journal of Virology, WASHINGTON, American Society for Microbiology, 2021, 0022-538X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.549

Kód RIV

RIV/00216224:14740/21:00119637

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1128/JVI.01950-20

UT WoS

000652658800002

Klíčová slova anglicky

RNA; capsid opening; genome release; iflavirus

Štítky

CF CRYO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 14:19, Ing. Martina Blahová

Anotace

V originále

Infections with Kashmir bee virus (KBV) are lethal for honeybees and have been associated with colony collapse disorder. KBV and closely related viruses contribute to the ongoing decline in the number of honeybee colonies in North America, Europe, Australia, and other parts of the world. Despite the economic and ecological impact of KBV, its structure and infection process remain unknown. Here, we present the structure of the virion of KBV determined to a resolution of 2.8 angstrom. We show that the exposure of KBV to acidic pH induces a reduction in interpentamer contacts within capsids and the reorganization of its RNA genome from a uniform distribution to regions of high and low density. Capsids of KBV crack into pieces at acidic pH, resulting in the formation of open particles lacking pentamers of capsid proteins. The large openings of capsids enable the rapid release of genomes and thus limit the probability of their degradation by RNases. The opening of capsids may be a shared mechanism for the genome release of viruses from the family Dicistroviridae. IMPORTANCE The western honeybee (Apis mellifera) is indispensable for maintaining agricultural productivity as well as the abundance and diversity of wild flowering plants. However, bees suffer from environmental pollution, parasites, and pathogens, including viruses. Outbreaks of virus infections cause the deaths of individual honeybees as well as collapses of whole colonies. Kashmir bee virus has been associated with colony collapse disorder in the United States, and no cure for the disease is currently available. Here, we report the structure of an infectious particle of Kashmir bee virus and show how its protein capsid opens to release the genome. Our structural characterization of the infection process determined that therapeutic compounds stabilizing contacts between pentamers of capsid proteins could prevent the genome release of the virus.

Návaznosti

GX19-25982X, projekt VaV

Název: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Grantová agentura ČR, Structural study of enterovirus replication in situ

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

MUKHAMEDOVA, Liya, Tibor FÜZIK, Jiří NOVÁČEK, Dominik HREBÍK, A. PRIDAL, G.A. MARTI, D.M.A. GUERIN a Pavel PLEVKA. Virion Structure and In Vitro Genome Release Mechanism of Dicistrovirus Kashmir Bee Virus. Journal of Virology. WASHINGTON: American Society for Microbiology, 2021, roč. 95, č. 11, s. nestrankovano, 11 s. ISSN 0022-538X. Dostupné z: https://dx.doi.org/10.1128/JVI.01950-20.

@article{1832006,
   author = {Mukhamedova, Liya and Füzik, Tibor and Nováček, Jiří and Hrebík, Dominik and Pridal, A. and Marti, G.A. and Guerin, D.M.A. and Plevka, Pavel},
   article_location = {WASHINGTON},
   article_number = {11},
   doi = {http://dx.doi.org/10.1128/JVI.01950-20},
   keywords = {RNA; capsid opening; genome release; iflavirus},
   language = {eng},
   issn = {0022-538X},
   journal = {Journal of Virology},
   title = {Virion Structure and In Vitro Genome Release Mechanism of Dicistrovirus Kashmir Bee Virus},
   url = {https://journals.asm.org/doi/10.1128/JVI.01950-20},
   volume = {95},
   year = {2021}
}

TY  - JOUR
ID  - 1832006
AU  - Mukhamedova, Liya - Füzik, Tibor - Nováček, Jiří - Hrebík, Dominik - Pridal, A. - Marti, G.A. - Guerin, D.M.A. - Plevka, Pavel
PY  - 2021
TI  - Virion Structure and In Vitro Genome Release Mechanism of Dicistrovirus Kashmir Bee Virus
JF  - Journal of Virology
VL  - 95
IS  - 11
SP  - nestrankovano
EP  - nestrankovano
PB  - American Society for Microbiology
SN  - 0022538X
KW  - RNA
KW  - capsid opening
KW  - genome release
KW  - iflavirus
UR  - https://journals.asm.org/doi/10.1128/JVI.01950-20
N2  - Infections with Kashmir bee virus (KBV) are lethal for honeybees and have been associated with colony collapse disorder. KBV and closely related viruses contribute to the ongoing decline in the number of honeybee colonies in North America, Europe, Australia, and other parts of the world. Despite the economic and ecological impact of KBV, its structure and infection process remain unknown. Here, we present the structure of the virion of KBV determined to a resolution of 2.8 angstrom. We show that the exposure of KBV to acidic pH induces a reduction in interpentamer contacts within capsids and the reorganization of its RNA genome from a uniform distribution to regions of high and low density. Capsids of KBV crack into pieces at acidic pH, resulting in the formation of open particles lacking pentamers of capsid proteins. The large openings of capsids enable the rapid release of genomes and thus limit the probability of their degradation by RNases. The opening of capsids may be a shared mechanism for the genome release of viruses from the family Dicistroviridae. IMPORTANCE The western honeybee (Apis mellifera) is indispensable for maintaining agricultural productivity as well as the abundance and diversity of wild flowering plants. However, bees suffer from environmental pollution, parasites, and pathogens, including viruses. Outbreaks of virus infections cause the deaths of individual honeybees as well as collapses of whole colonies. Kashmir bee virus has been associated with colony collapse disorder in the United States, and no cure for the disease is currently available. Here, we report the structure of an infectious particle of Kashmir bee virus and show how its protein capsid opens to release the genome. Our structural characterization of the infection process determined that therapeutic compounds stabilizing contacts between pentamers of capsid proteins could prevent the genome release of the virus.
ER  -

MUKHAMEDOVA, Liya, Tibor FÜZIK, Jiří NOVÁČEK, Dominik HREBÍK, A. PRIDAL, G.A. MARTI, D.M.A. GUERIN a Pavel PLEVKA. Virion Structure and In Vitro Genome Release Mechanism of Dicistrovirus Kashmir Bee Virus. \textit{Journal of Virology}. WASHINGTON: American Society for Microbiology, 2021, roč.~95, č.~11, s.~nestrankovano, 11 s. ISSN~0022-538X. Dostupné z: https://dx.doi.org/10.1128/JVI.01950-20.

Podrobný výpis o publikaci