Occurrence of cylindrospermopsin, anatoxin-a and their homologs
in the southern Czech Republic-Taxonomical, analytical, and
molecular approaches

J 2021

Occurrence of cylindrospermopsin, anatoxin-a and their homologs in the southern Czech Republic-Taxonomical, analytical, and molecular approaches

BLÁHOVÁ, Lucie, Luděk SEHNAL, Olga LEPSOVA SKACELOVA, Vendula SZMUCOVÁ, Pavel BABICA et. al.

Základní údaje

Originální název

Occurrence of cylindrospermopsin, anatoxin-a and their homologs in the southern Czech Republic-Taxonomical, analytical, and molecular approaches

Autoři

BLÁHOVÁ, Lucie (203 Česká republika, domácí), Luděk SEHNAL (203 Česká republika, domácí), Olga LEPSOVA SKACELOVA (203 Česká republika), Vendula SZMUCOVÁ (203 Česká republika, domácí), Pavel BABICA (203 Česká republika, domácí), Klára HILSCHEROVÁ (203 Česká republika, domácí), Jonna TEIKARI (246 Finsko), Kaarina SIVONEN (246 Finsko) a Luděk BLÁHA (203 Česká republika, garant, domácí)

Vydání

Harmful Algae, AMSTERDAM, Elsevier Science, 2021, 1568-9883

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10617 Marine biology, freshwater biology, limnology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.905

Kód RIV

RIV/00216224:14310/21:00119640

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.hal.2021.102101

UT WoS

000698761100005

Klíčová slova anglicky

Anatoxin-a; Homoanatoxin; Cylindrospermopsin; cyrJ gene; anaC gene; Cyanotoxin producers

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2022 11:08, Mgr. Marie Šípková, DiS.

Anotace

V originále

Water bloom-forming cyanobacteria have a severe impact on freshwater quality. Although some cyanobacterial toxins such as microcystins have been studied extensively, other toxins like anatoxin-a (ATX) and their structural analogs - as well as cyanobacterial taxa producing these toxins remain to be explored in detail. The present study investigated levels of ATX, CYN and their homologs along with the occurrence of anaC and cyrJ genes in water blooms in 16 sites in the Czech Republic that were pre-selected concerning the presence of potential toxin producers. Besides, we also studied toxins and genes in a series of strains available in our laboratories. ATX and its congener HATX were detected in 5 natural biomass samples from the Czech Republic (maximum concentration 2.8 micrograms per gram d.w.). Interestingly, the anaC gene coding for ATX production was not detected in any of these toxin-positive biomass samples. The concentrations of ATX congeners in cyanobacterial laboratory strains were about 10-times higher than those of the original ATX, which calls for further research addressing levels and hazards of ATX analogs. Regarding the CYN and 7-deoxyCYN (other CYN congeners were not analyzed in this study) - these toxins were identified in a single small pond in the Czech Republic at concentrations 4.3 and 2.7 micrograms per gram of biomass d.w., respectively (corresponded to dissolved concentrations higher than 1 microgram per liter). The CYN-positive sample was dominated by CYN-producing taxa Raphidiopsis (basionym Cylindrospermopsis) and Cuspidothrix. We also confirmed the presence of a specific cyrJ gene in this natural bloom sample. To our knowledge, this is the first study pointing to Raphidiopsis (Cylindrospermopsis) and Cuspidothrix as producers of CYN in Europe. This observation calls for further research because of their increasing occurrence in (Central) Europe along with the global change. The present study demonstrates the importance of using combined (taxonomical, analytical, and molecular) approaches in the assessment of hazardous cyanobacteria and their toxins in freshwaters.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

GA18-15199S, projekt VaV

Název: Bioaktivní látky ze sinic ovlivňující signálování jaderných receptorů a vývoj obratlovců

Investor: Grantová agentura ČR, Bioaktivní látky ze sinic ovlivňující signálování jaderných receptorů a vývoj obratlovců

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

Citovat

BLÁHOVÁ, Lucie, Luděk SEHNAL, Olga LEPSOVA SKACELOVA, Vendula SZMUCOVÁ, Pavel BABICA, Klára HILSCHEROVÁ, Jonna TEIKARI, Kaarina SIVONEN a Luděk BLÁHA. Occurrence of cylindrospermopsin, anatoxin-a and their homologs in the southern Czech Republic-Taxonomical, analytical, and molecular approaches. Harmful Algae. AMSTERDAM: Elsevier Science, 2021, roč. 108, August 2021, s. 1-9. ISSN 1568-9883. Dostupné z: https://dx.doi.org/10.1016/j.hal.2021.102101.

@article{1832439,
   author = {Bláhová, Lucie and Sehnal, Luděk and Lepsova Skacelova, Olga and Szmucová, Vendula and Babica, Pavel and Hilscherová, Klára and Teikari, Jonna and Sivonen, Kaarina and Bláha, Luděk},
   article_location = {AMSTERDAM},
   article_number = {August 2021},
   doi = {http://dx.doi.org/10.1016/j.hal.2021.102101},
   keywords = {Anatoxin-a; Homoanatoxin; Cylindrospermopsin; cyrJ gene; anaC gene; Cyanotoxin producers},
   language = {eng},
   issn = {1568-9883},
   journal = {Harmful Algae},
   title = {Occurrence of cylindrospermopsin, anatoxin-a and their homologs in the southern Czech Republic-Taxonomical, analytical, and molecular approaches},
   url = {https://www.sciencedirect.com/science/article/pii/S1568988321001311?via%3Dihub},
   volume = {108},
   year = {2021}
}

TY  - JOUR
ID  - 1832439
AU  - Bláhová, Lucie - Sehnal, Luděk - Lepsova Skacelova, Olga - Szmucová, Vendula - Babica, Pavel - Hilscherová, Klára - Teikari, Jonna - Sivonen, Kaarina - Bláha, Luděk
PY  - 2021
TI  - Occurrence of cylindrospermopsin, anatoxin-a and their homologs in the southern Czech Republic-Taxonomical, analytical, and molecular approaches
JF  - Harmful Algae
VL  - 108
IS  - August 2021
SP  - 1-9
EP  - 1-9
PB  - Elsevier Science
SN  - 15689883
KW  - Anatoxin-a
KW  - Homoanatoxin
KW  - Cylindrospermopsin
KW  - cyrJ gene
KW  - anaC gene
KW  - Cyanotoxin producers
UR  - https://www.sciencedirect.com/science/article/pii/S1568988321001311?via%3Dihub
N2  - Water bloom-forming cyanobacteria have a severe impact on freshwater quality. Although some cyanobacterial toxins such as microcystins have been studied extensively, other toxins like anatoxin-a (ATX) and their structural analogs - as well as cyanobacterial taxa producing these toxins remain to be explored in detail. The present study investigated levels of ATX, CYN and their homologs along with the occurrence of anaC and cyrJ genes in water blooms in 16 sites in the Czech Republic that were pre-selected concerning the presence of potential toxin producers. Besides, we also studied toxins and genes in a series of strains available in our laboratories. ATX and its congener HATX were detected in 5 natural biomass samples from the Czech Republic (maximum concentration 2.8 micrograms per gram d.w.). Interestingly, the anaC gene coding for ATX production was not detected in any of these toxin-positive biomass samples. The concentrations of ATX congeners in cyanobacterial laboratory strains were about 10-times higher than those of the original ATX, which calls for further research addressing levels and hazards of ATX analogs. Regarding the CYN and 7-deoxyCYN (other CYN congeners were not analyzed in this study) - these toxins were identified in a single small pond in the Czech Republic at concentrations 4.3 and 2.7 micrograms per gram of biomass d.w., respectively (corresponded to dissolved concentrations higher than 1 microgram per liter). The CYN-positive sample was dominated by CYN-producing taxa Raphidiopsis (basionym Cylindrospermopsis) and Cuspidothrix. We also confirmed the presence of a specific cyrJ gene in this natural bloom sample. To our knowledge, this is the first study pointing to Raphidiopsis (Cylindrospermopsis) and Cuspidothrix as producers of CYN in Europe. This observation calls for further research because of their increasing occurrence in (Central) Europe along with the global change. The present study demonstrates the importance of using combined (taxonomical, analytical, and molecular) approaches in the assessment of hazardous cyanobacteria and their toxins in freshwaters.
ER  -

BLÁHOVÁ, Lucie, Luděk SEHNAL, Olga LEPSOVA SKACELOVA, Vendula SZMUCOVÁ, Pavel BABICA, Klára HILSCHEROVÁ, Jonna TEIKARI, Kaarina SIVONEN a Luděk BLÁHA. Occurrence of cylindrospermopsin, anatoxin-a and their homologs in the southern Czech Republic-Taxonomical, analytical, and molecular approaches. \textit{Harmful Algae}. AMSTERDAM: Elsevier Science, 2021, roč.~108, August 2021, s.~1-9. ISSN~1568-9883. Dostupné z: https://dx.doi.org/10.1016/j.hal.2021.102101.

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