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@article{1834647,
author = {Capkova, Nikola and Pospíšilová, Veronika and Fedorová, Veronika and Raška, Jan and Pospisilova, Katerina and Dal Ben, Matteo and Dvorak, Ales and Viktorova, Jitka and Bohačiaková, Dáša and Vitek, Libor},
article_location = {Basel},
article_number = {10},
doi = {http://dx.doi.org/10.3390/antiox10101532},
keywords = {bilirubin; neurodevelopment; phototherapy},
language = {eng},
issn = {2076-3921},
journal = {Antioxidants},
title = {The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells},
url = {https://www.mdpi.com/2076-3921/10/10/1532},
volume = {10},
year = {2021}
}
TY - JOUR
ID - 1834647
AU - Capkova, Nikola - Pospíšilová, Veronika - Fedorová, Veronika - Raška, Jan - Pospisilova, Katerina - Dal Ben, Matteo - Dvorak, Ales - Viktorova, Jitka - Bohačiaková, Dáša - Vitek, Libor
PY - 2021
TI - The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells
JF - Antioxidants
VL - 10
IS - 10
SP - 1-16
EP - 1-16
PB - MDPI
SN - 20763921
KW - bilirubin
KW - neurodevelopment
KW - phototherapy
UR - https://www.mdpi.com/2076-3921/10/10/1532
N2 - The 'gold standard' treatment of severe neonatal jaundice is phototherapy with blue-green light, which produces more polar photo-oxidation products that are easily excreted via the bile or urine. The aim of this study was to compare the effects of bilirubin (BR) and its major photo-oxidation product lumirubin (LR) on the proliferation, differentiation, morphology, and specific gene and protein expressions of self-renewing human pluripotent stem cell-derived neural stem cells (NSC). Neither BR nor LR in biologically relevant concentrations (12.5 and 25 mu mol/L) affected cell proliferation or the cell cycle phases of NSC. Although none of these pigments affected terminal differentiation to neurons and astrocytes, when compared to LR, BR exerted a dose-dependent cytotoxicity on self-renewing NSC. In contrast, LR had a substantial effect on the morphology of the NSC, inducing them to form highly polar rosette-like structures associated with the redistribution of specific cellular proteins (beta-catenin/N-cadherin) responsible for membrane polarity. This observation was accompanied by lower expressions of NSC-specific proteins (such as SOX1, NR2F2, or PAX6) together with the upregulation of phospho-ERK. Collectively, the data indicated that both BR and LR affect early human neurodevelopment in vitro, which may have clinical relevance in phototherapy-treated hyperbilirubinemic neonates.
ER -
CAPKOVA, Nikola, Veronika POSPÍŠILOVÁ, Veronika FEDOROVÁ, Jan RAŠKA, Katerina POSPISILOVA, Matteo DAL BEN, Ales DVORAK, Jitka VIKTOROVA, Dáša BOHAČIAKOVÁ a Libor VITEK. The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells. \textit{Antioxidants}. Basel: MDPI, 2021, roč.~10, č.~10, s.~1-16. ISSN~2076-3921. Dostupné z: https://dx.doi.org/10.3390/antiox10101532.
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