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@article{1834647, author = {Capkova, Nikola and Pospíšilová, Veronika and Fedorová, Veronika and Raška, Jan and Pospisilova, Katerina and Dal Ben, Matteo and Dvorak, Ales and Viktorova, Jitka and Bohačiaková, Dáša and Vitek, Libor}, article_location = {Basel}, article_number = {10}, doi = {http://dx.doi.org/10.3390/antiox10101532}, keywords = {bilirubin; neurodevelopment; phototherapy}, language = {eng}, issn = {2076-3921}, journal = {Antioxidants}, title = {The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells}, url = {https://www.mdpi.com/2076-3921/10/10/1532}, volume = {10}, year = {2021} }
TY - JOUR ID - 1834647 AU - Capkova, Nikola - Pospíšilová, Veronika - Fedorová, Veronika - Raška, Jan - Pospisilova, Katerina - Dal Ben, Matteo - Dvorak, Ales - Viktorova, Jitka - Bohačiaková, Dáša - Vitek, Libor PY - 2021 TI - The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells JF - Antioxidants VL - 10 IS - 10 SP - 1-16 EP - 1-16 PB - MDPI SN - 20763921 KW - bilirubin KW - neurodevelopment KW - phototherapy UR - https://www.mdpi.com/2076-3921/10/10/1532 N2 - The 'gold standard' treatment of severe neonatal jaundice is phototherapy with blue-green light, which produces more polar photo-oxidation products that are easily excreted via the bile or urine. The aim of this study was to compare the effects of bilirubin (BR) and its major photo-oxidation product lumirubin (LR) on the proliferation, differentiation, morphology, and specific gene and protein expressions of self-renewing human pluripotent stem cell-derived neural stem cells (NSC). Neither BR nor LR in biologically relevant concentrations (12.5 and 25 mu mol/L) affected cell proliferation or the cell cycle phases of NSC. Although none of these pigments affected terminal differentiation to neurons and astrocytes, when compared to LR, BR exerted a dose-dependent cytotoxicity on self-renewing NSC. In contrast, LR had a substantial effect on the morphology of the NSC, inducing them to form highly polar rosette-like structures associated with the redistribution of specific cellular proteins (beta-catenin/N-cadherin) responsible for membrane polarity. This observation was accompanied by lower expressions of NSC-specific proteins (such as SOX1, NR2F2, or PAX6) together with the upregulation of phospho-ERK. Collectively, the data indicated that both BR and LR affect early human neurodevelopment in vitro, which may have clinical relevance in phototherapy-treated hyperbilirubinemic neonates. ER -
CAPKOVA, Nikola, Veronika POSPÍŠILOVÁ, Veronika FEDOROVÁ, Jan RAŠKA, Katerina POSPISILOVA, Matteo DAL BEN, Ales DVORAK, Jitka VIKTOROVA, Dáša BOHAČIAKOVÁ a Libor VITEK. The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells. \textit{Antioxidants}. Basel: MDPI, 2021, roč.~10, č.~10, s.~1-16. ISSN~2076-3921. Dostupné z: https://dx.doi.org/10.3390/antiox10101532.
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