The Effects of Bilirubin and Lumirubin on the Differentiation
of Human Pluripotent Cell-Derived Neural Stem Cells

J 2021

The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells

CAPKOVA, Nikola; Veronika POSPÍŠILOVÁ; Veronika FEDOROVÁ; Jan RAŠKA; Katerina POSPISILOVA et. al.

Základní údaje

Originální název

The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells

Autoři

CAPKOVA, Nikola (203 Česká republika); Veronika POSPÍŠILOVÁ (203 Česká republika, domácí); Veronika FEDOROVÁ (203 Česká republika, domácí); Jan RAŠKA (203 Česká republika, domácí); Katerina POSPISILOVA (203 Česká republika); Matteo DAL BEN; Ales DVORAK (203 Česká republika); Jitka VIKTOROVA (203 Česká republika); Dáša BOHAČIAKOVÁ (703 Slovensko, domácí) a Libor VITEK (203 Česká republika, garant)

Vydání

Antioxidants, Basel, MDPI, 2021, 2076-3921

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.675

Kód RIV

RIV/00216224:14110/21:00119650

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.3390/antiox10101532

UT WoS

000712298000001

EID Scopus

2-s2.0-85115777407

Klíčová slova anglicky

bilirubin; neurodevelopment; phototherapy

Štítky

14110517, CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 5. 2025 13:04, Mgr. Tereza Miškechová

Anotace

V originále

The 'gold standard' treatment of severe neonatal jaundice is phototherapy with blue-green light, which produces more polar photo-oxidation products that are easily excreted via the bile or urine. The aim of this study was to compare the effects of bilirubin (BR) and its major photo-oxidation product lumirubin (LR) on the proliferation, differentiation, morphology, and specific gene and protein expressions of self-renewing human pluripotent stem cell-derived neural stem cells (NSC). Neither BR nor LR in biologically relevant concentrations (12.5 and 25 mu mol/L) affected cell proliferation or the cell cycle phases of NSC. Although none of these pigments affected terminal differentiation to neurons and astrocytes, when compared to LR, BR exerted a dose-dependent cytotoxicity on self-renewing NSC. In contrast, LR had a substantial effect on the morphology of the NSC, inducing them to form highly polar rosette-like structures associated with the redistribution of specific cellular proteins (beta-catenin/N-cadherin) responsible for membrane polarity. This observation was accompanied by lower expressions of NSC-specific proteins (such as SOX1, NR2F2, or PAX6) together with the upregulation of phospho-ERK. Collectively, the data indicated that both BR and LR affect early human neurodevelopment in vitro, which may have clinical relevance in phototherapy-treated hyperbilirubinemic neonates.

Návaznosti

GA21-01799S, projekt VaV

Název: Flavonoidy jako unikátní třída molekul fotochemicky uvolňujících oxid uhelnatý

Investor: Grantová agentura ČR, Flavonoidy jako unikátní třída molekul fotochemicky uvolňujících oxid uhelnatý

GJ18-25429Y, projekt VaV

Název: Funkční studie mikroRNA u nerálních kmenových buněk v průběhu diferenciace

Investor: Grantová agentura ČR, Funkční studie mikroRNA u nerálních kmenových buněk v průběhu diferenciace

NV18-07-00342, projekt VaV

Název: Studium degradačních produktů bilirubinu vznikajících při fototerapii novorozenecké žloutenky

Investor: Ministerstvo zdravotnictví ČR, Studium degradačních produktů bilirubinu vznikajících při fototerapii novorozenecké žloutenky

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

CAPKOVA, Nikola; Veronika POSPÍŠILOVÁ; Veronika FEDOROVÁ; Jan RAŠKA; Katerina POSPISILOVA; Matteo DAL BEN; Ales DVORAK; Jitka VIKTOROVA; Dáša BOHAČIAKOVÁ a Libor VITEK. The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells. Antioxidants. Basel: MDPI, 2021, roč. 10, č. 10, s. 1-16. ISSN 2076-3921. Dostupné z: https://doi.org/10.3390/antiox10101532.

@article{1834647,
   author = {Capkova, Nikola and Pospíšilová, Veronika and Fedorová, Veronika and Raška, Jan and Pospisilova, Katerina and Dal Ben, Matteo and Dvorak, Ales and Viktorova, Jitka and Bohačiaková, Dáša and Vitek, Libor},
   article_location = {Basel},
   article_number = {10},
   doi = {https://doi.org/10.3390/antiox10101532},
   keywords = {bilirubin; neurodevelopment; phototherapy},
   language = {eng},
   issn = {2076-3921},
   journal = {Antioxidants},
   title = {The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells},
   url = {https://www.mdpi.com/2076-3921/10/10/1532},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1834647
AU  - Capkova, Nikola - Pospíšilová, Veronika - Fedorová, Veronika - Raška, Jan - Pospisilova, Katerina - Dal Ben, Matteo - Dvorak, Ales - Viktorova, Jitka - Bohačiaková, Dáša - Vitek, Libor
PY  - 2021
TI  - The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells
JF  - Antioxidants
VL  - 10
IS  - 10
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 20763921
KW  - bilirubin
KW  - neurodevelopment
KW  - phototherapy
UR  - https://www.mdpi.com/2076-3921/10/10/1532
N2  - The 'gold standard' treatment of severe neonatal jaundice is phototherapy with blue-green light, which produces more polar photo-oxidation products that are easily excreted via the bile or urine. The aim of this study was to compare the effects of bilirubin (BR) and its major photo-oxidation product lumirubin (LR) on the proliferation, differentiation, morphology, and specific gene and protein expressions of self-renewing human pluripotent stem cell-derived neural stem cells (NSC). Neither BR nor LR in biologically relevant concentrations (12.5 and 25 mu mol/L) affected cell proliferation or the cell cycle phases of NSC. Although none of these pigments affected terminal differentiation to neurons and astrocytes, when compared to LR, BR exerted a dose-dependent cytotoxicity on self-renewing NSC. In contrast, LR had a substantial effect on the morphology of the NSC, inducing them to form highly polar rosette-like structures associated with the redistribution of specific cellular proteins (beta-catenin/N-cadherin) responsible for membrane polarity. This observation was accompanied by lower expressions of NSC-specific proteins (such as SOX1, NR2F2, or PAX6) together with the upregulation of phospho-ERK. Collectively, the data indicated that both BR and LR affect early human neurodevelopment in vitro, which may have clinical relevance in phototherapy-treated hyperbilirubinemic neonates.
ER  -

CAPKOVA, Nikola; Veronika POSPÍŠILOVÁ; Veronika FEDOROVÁ; Jan RAŠKA; Katerina POSPISILOVA; Matteo DAL BEN; Ales DVORAK; Jitka VIKTOROVA; Dáša BOHAČIAKOVÁ a Libor VITEK. The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells. \textit{Antioxidants}. Basel: MDPI, 2021, roč.~10, č.~10, s.~1-16. ISSN~2076-3921. Dostupné z: https://doi.org/10.3390/antiox10101532.

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