Variety of size and form of GRM2 bacterial microcompartment
particles

J 2021

Variety of size and form of GRM2 bacterial microcompartment particles

CESLE, E.E., Anatolij FILIMONĚNKO, K. TARS a G. KALNINS

Základní údaje

Originální název

Variety of size and form of GRM2 bacterial microcompartment particles

Autoři

CESLE, E.E., Anatolij FILIMONĚNKO (203 Česká republika, garant, domácí), K. TARS a G. KALNINS

Vydání

Protein Science, HOBOKEN, JOHN WILEY & SONS INC, 2021, 0961-8368

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.993

Kód RIV

RIV/00216224:14740/21:00124188

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/pro.4069

UT WoS

000635930300001

Klíčová slova anglicky

bacterial microcompartments; cryo‐ EM; GRM2; Klebsiella pneumoniae

Štítky

CF CRYO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 14:13, Ing. Martina Blahová

Anotace

V originále

Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi-permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cell from harmful intermediates. With the ability to encapsulate non-native enzymes, BMCs show high potential for applied use. For this goal, a detailed look into shell form variability is significant to predict shell adaptability. Here we present four novel 3D cryo-EM maps of recombinant Klebsiella pneumoniae GRM2 BMC shell particles with the resolution in range of 9 to 22 angstrom and nine novel 2D classes corresponding to discrete BMC shell forms. These structures reveal icosahedral, elongated, oblate, multi-layered and polyhedral traits of BMCs, indicating considerable variation in size and form as well as adaptability during shell formation processes.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

CESLE, E.E., Anatolij FILIMONĚNKO, K. TARS a G. KALNINS. Variety of size and form of GRM2 bacterial microcompartment particles. Protein Science. HOBOKEN: JOHN WILEY & SONS INC, 2021, roč. 30, č. 5, s. 1035-1043. ISSN 0961-8368. Dostupné z: https://dx.doi.org/10.1002/pro.4069.

@article{1834740,
   author = {Cesle, E.E. and Filimoněnko, Anatolij and Tars, K. and Kalnins, G.},
   article_location = {HOBOKEN},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/pro.4069},
   keywords = {bacterial microcompartments; cryo‐ EM; GRM2; Klebsiella pneumoniae},
   language = {eng},
   issn = {0961-8368},
   journal = {Protein Science},
   title = {Variety of size and form of GRM2 bacterial microcompartment particles},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/pro.4069},
   volume = {30},
   year = {2021}
}

TY  - JOUR
ID  - 1834740
AU  - Cesle, E.E. - Filimoněnko, Anatolij - Tars, K. - Kalnins, G.
PY  - 2021
TI  - Variety of size and form of GRM2 bacterial microcompartment particles
JF  - Protein Science
VL  - 30
IS  - 5
SP  - 1035-1043
EP  - 1035-1043
PB  - JOHN WILEY & SONS INC
SN  - 09618368
KW  - bacterial microcompartments
KW  - cryo‐ EM
KW  - GRM2
KW  - Klebsiella pneumoniae
UR  - https://onlinelibrary.wiley.com/doi/10.1002/pro.4069
N2  - Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi-permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cell from harmful intermediates. With the ability to encapsulate non-native enzymes, BMCs show high potential for applied use. For this goal, a detailed look into shell form variability is significant to predict shell adaptability. Here we present four novel 3D cryo-EM maps of recombinant Klebsiella pneumoniae GRM2 BMC shell particles with the resolution in range of 9 to 22 angstrom and nine novel 2D classes corresponding to discrete BMC shell forms. These structures reveal icosahedral, elongated, oblate, multi-layered and polyhedral traits of BMCs, indicating considerable variation in size and form as well as adaptability during shell formation processes.
ER  -

CESLE, E.E., Anatolij FILIMONĚNKO, K. TARS a G. KALNINS. Variety of size and form of GRM2 bacterial microcompartment particles. \textit{Protein Science}. HOBOKEN: JOHN WILEY \&{}amp; SONS INC, 2021, roč.~30, č.~5, s.~1035-1043. ISSN~0961-8368. Dostupné z: https://dx.doi.org/10.1002/pro.4069.

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