Functional plasma-polymerized hydrogel coatings for
electrochemical biosensing

J 2022

Functional plasma-polymerized hydrogel coatings for electrochemical biosensing

LEVIEN, Monique, Zdeněk FARKA, Matěj PASTUCHA, Petr SKLÁDAL, Zahra NASRI et. al.

Základní údaje

Originální název

Functional plasma-polymerized hydrogel coatings for electrochemical biosensing

Autoři

LEVIEN, Monique (276 Německo), Zdeněk FARKA (203 Česká republika, garant, domácí), Matěj PASTUCHA (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí), Zahra NASRI, Klaus-Dieter WELTMANN a Katja FRICKE

Vydání

Applied Surface Science, Elsevier, 2022, 0169-4332

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.700

Kód RIV

RIV/00216224:14310/22:00125397

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.apsusc.2022.152511

UT WoS

000773715400002

Klíčová slova anglicky

Hydrogel coating; Functional surface; Atmospheric pressure plasma polymerization; Electrochemical biosensor; Amperometry; Glucose oxidase; Acetylcholinesterase

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 1. 2023 11:50, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Acrylate-based hydrogels with multifunctional properties have proven to be suitable candidates for the development of sensor systems. They gained popularity especially in combination with bioelectronics, as there is a need to understand and control the interactions of bionic devices with the human body and other environments. In this study, we present results on the biointeraction capability of plasma-polymerized (pp) hydrogels made of hydroxyethyl methacrylate (HEMA) and 2-(diethylamino)ethyl methacrylate (DEAEMA) mixtures on gold screen-printed electrodes (SPE). The hydrogels were generated by an atmospheric pressure plasma jet, and their chemical composition was characterized via FT-IR. The FT-IR analysis revealed several functional groups suitable for biomolecule immobilization, whereas the amount of -C-N, –OH, and -C-O-C groups differs depending on the mixture ratios. The pp HEMA:DEAEMA (HD) hydrogel coatings provide alternative interfacing materials for electrochemical biosensing. The enzymes glucose oxidase (GOx) and acetylcholinesterase (AChE) were coupled to the hydrogel-based surfaces, and the effects of the mixture ratios on the biomolecule immobilization were investigated. It is possible to address different functional groups of the mixtures with different immobilization strategies; thus, the sensor response can be optimized. Finally, glucose as GOx substrate and eserine as AChE inhibitor were detected by amperometry to demonstrate the practical biosensing applicability of the coatings.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

LEVIEN, Monique, Zdeněk FARKA, Matěj PASTUCHA, Petr SKLÁDAL, Zahra NASRI, Klaus-Dieter WELTMANN a Katja FRICKE. Functional plasma-polymerized hydrogel coatings for electrochemical biosensing. Applied Surface Science. Elsevier, 2022, roč. 584, May, s. 1-8. ISSN 0169-4332. Dostupné z: https://dx.doi.org/10.1016/j.apsusc.2022.152511.

@article{1834920,
   author = {Levien, Monique and Farka, Zdeněk and Pastucha, Matěj and Skládal, Petr and Nasri, Zahra and Weltmann, KlausandDieter and Fricke, Katja},
   article_number = {May},
   doi = {http://dx.doi.org/10.1016/j.apsusc.2022.152511},
   keywords = {Hydrogel coating; Functional surface; Atmospheric pressure plasma polymerization; Electrochemical biosensor; Amperometry; Glucose oxidase; Acetylcholinesterase},
   language = {eng},
   issn = {0169-4332},
   journal = {Applied Surface Science},
   title = {Functional plasma-polymerized hydrogel coatings for electrochemical biosensing},
   url = {https://www.sciencedirect.com/science/article/abs/pii/S0169433222000964},
   volume = {584},
   year = {2022}
}

TY  - JOUR
ID  - 1834920
AU  - Levien, Monique - Farka, Zdeněk - Pastucha, Matěj - Skládal, Petr - Nasri, Zahra - Weltmann, Klaus-Dieter - Fricke, Katja
PY  - 2022
TI  - Functional plasma-polymerized hydrogel coatings for electrochemical biosensing
JF  - Applied Surface Science
VL  - 584
IS  - May
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 01694332
KW  - Hydrogel coating
KW  - Functional surface
KW  - Atmospheric pressure plasma polymerization
KW  - Electrochemical biosensor
KW  - Amperometry
KW  - Glucose oxidase
KW  - Acetylcholinesterase
UR  - https://www.sciencedirect.com/science/article/abs/pii/S0169433222000964
N2  - Acrylate-based hydrogels with multifunctional properties have proven to be suitable candidates for the development of sensor systems. They gained popularity especially in combination with bioelectronics, as there is a need to understand and control the interactions of bionic devices with the human body and other environments. In this study, we present results on the biointeraction capability of plasma-polymerized (pp) hydrogels made of hydroxyethyl methacrylate (HEMA) and 2-(diethylamino)ethyl methacrylate (DEAEMA) mixtures on gold screen-printed electrodes (SPE). The hydrogels were generated by an atmospheric pressure plasma jet, and their chemical composition was characterized via FT-IR. The FT-IR analysis revealed several functional groups suitable for biomolecule immobilization, whereas the amount of -C-N, –OH, and -C-O-C groups differs depending on the mixture ratios. The pp HEMA:DEAEMA (HD) hydrogel coatings provide alternative interfacing materials for electrochemical biosensing. The enzymes glucose oxidase (GOx) and acetylcholinesterase (AChE) were coupled to the hydrogel-based surfaces, and the effects of the mixture ratios on the biomolecule immobilization were investigated. It is possible to address different functional groups of the mixtures with different immobilization strategies; thus, the sensor response can be optimized. Finally, glucose as GOx substrate and eserine as AChE inhibitor were detected by amperometry to demonstrate the practical biosensing applicability of the coatings.
ER  -

LEVIEN, Monique, Zdeněk FARKA, Matěj PASTUCHA, Petr SKLÁDAL, Zahra NASRI, Klaus-Dieter WELTMANN a Katja FRICKE. Functional plasma-polymerized hydrogel coatings for electrochemical biosensing. \textit{Applied Surface Science}. Elsevier, 2022, roč.~584, May, s.~1-8. ISSN~0169-4332. Dostupné z: https://dx.doi.org/10.1016/j.apsusc.2022.152511.

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