1H NMR Profiling of Honey Bee Bodies Revealed Metabolic
Differences between Summer and Winter Bees

J 2022

1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees

LEE, Saetbyeol, Filip KALCIC, Iola F. DUARTE, Dalibor TITERA, Martin KAMLER et. al.

Základní údaje

Originální název

1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees

Autoři

LEE, Saetbyeol, Filip KALCIC, Iola F. DUARTE, Dalibor TITERA, Martin KAMLER, Pavel MRNA, Pavel HYRŠL (203 Česká republika, domácí), Jiri DANIHLIK, Pavel DOBEŠ (203 Česká republika, domácí), Martin KUNC (203 Česká republika, domácí), Anna PUDLO a Jaroslav HAVLIK (garant)

Vydání

Insects, MDPI, 2022, 2075-4450

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10616 Entomology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.000

Kód RIV

RIV/00216224:14310/22:00119768

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/insects13020193

UT WoS

000769716500001

Klíčová slova anglicky

Apis mellifera; winter bees; nuclear magnetic resonance; metabolome; longevity

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2022 10:53, Mgr. Marie Šípková, DiS.

Anotace

V originále

In temperate climates, honey bee workers of the species Apis mellifera have different lifespans depending on the seasonal phenotype: summer bees (short lifespan) and winter bees (long lifespan). Many studies have revealed the biochemical parameters involved in the lifespan differentiation of summer and winter bees. However, comprehensive information regarding the metabolic changes occurring in their bodies between the two is limited. This study used proton nuclear magnetic resonance (1H NMR) spectroscopy to analyze the metabolic differences between summer and winter bees of the same age. The multivariate analysis showed that summer and winter bees could be distinguished based on their metabolic profiles. Among the 36 metabolites found, 28 metabolites have displayed significant changes from summer to winter bees. Compared to summer bees, trehalose in winter bees showed 1.9 times higher concentration, and all amino acids except for proline and alanine showed decreased patterns. We have also detected an unknown compound, with a CH3 singlet at 2.83 ppm, which is a potential biomarker that is about 13 times higher in summer bees. Our results show that the metabolites in summer and winter bees have distinctive characteristics; this information could provide new insights and support further studies on honey bee longevity and overwintering.

Návaznosti

QJ1610248, projekt VaV

Název: Dlouhověkost včel a její úloha v udržitelném chovu (Akronym: Dlouhověkost)

Investor: Ministerstvo zemědělství ČR, Dlouhověkost včel a její úloha v udržitelném chovu

QK1910286, projekt VaV

Název: Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

Investor: Ministerstvo zemědělství ČR, Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

Citovat

LEE, Saetbyeol, Filip KALCIC, Iola F. DUARTE, Dalibor TITERA, Martin KAMLER, Pavel MRNA, Pavel HYRŠL, Jiri DANIHLIK, Pavel DOBEŠ, Martin KUNC, Anna PUDLO a Jaroslav HAVLIK. 1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees. Insects. MDPI, 2022, roč. 13, č. 2, s. 1-13. ISSN 2075-4450. Dostupné z: https://dx.doi.org/10.3390/insects13020193.

@article{1835003,
   author = {Lee, Saetbyeol and Kalcic, Filip and Duarte, Iola F. and Titera, Dalibor and Kamler, Martin and Mrna, Pavel and Hyršl, Pavel and Danihlik, Jiri and Dobeš, Pavel and Kunc, Martin and Pudlo, Anna and Havlik, Jaroslav},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/insects13020193},
   keywords = {Apis mellifera; winter bees; nuclear magnetic resonance; metabolome; longevity},
   language = {eng},
   issn = {2075-4450},
   journal = {Insects},
   title = {1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees},
   url = {https://www.mdpi.com/2075-4450/13/2/193},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 1835003
AU  - Lee, Saetbyeol - Kalcic, Filip - Duarte, Iola F. - Titera, Dalibor - Kamler, Martin - Mrna, Pavel - Hyršl, Pavel - Danihlik, Jiri - Dobeš, Pavel - Kunc, Martin - Pudlo, Anna - Havlik, Jaroslav
PY  - 2022
TI  - 1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees
JF  - Insects
VL  - 13
IS  - 2
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 20754450
KW  - Apis mellifera
KW  - winter bees
KW  - nuclear magnetic resonance
KW  - metabolome
KW  - longevity
UR  - https://www.mdpi.com/2075-4450/13/2/193
N2  - In temperate climates, honey bee workers of the species Apis mellifera have different lifespans depending on the seasonal phenotype: summer bees (short lifespan) and winter bees (long lifespan). Many studies have revealed the biochemical parameters involved in the lifespan differentiation of summer and winter bees. However, comprehensive information regarding the metabolic changes occurring in their bodies between the two is limited. This study used proton nuclear magnetic resonance (1H NMR) spectroscopy to analyze the metabolic differences between summer and winter bees of the same age. The multivariate analysis showed that summer and winter bees could be distinguished based on their metabolic profiles. Among the 36 metabolites found, 28 metabolites have displayed significant changes from summer to winter bees. Compared to summer bees, trehalose in winter bees showed 1.9 times higher concentration, and all amino acids except for proline and alanine showed decreased patterns. We have also detected an unknown compound, with a CH3 singlet at 2.83 ppm, which is a potential biomarker that is about 13 times higher in summer bees. Our results show that the metabolites in summer and winter bees have distinctive characteristics; this information could provide new insights and support further studies on honey bee longevity and overwintering.
ER  -

LEE, Saetbyeol, Filip KALCIC, Iola F. DUARTE, Dalibor TITERA, Martin KAMLER, Pavel MRNA, Pavel HYRŠL, Jiri DANIHLIK, Pavel DOBEŠ, Martin KUNC, Anna PUDLO a Jaroslav HAVLIK. 1H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees. \textit{Insects}. MDPI, 2022, roč.~13, č.~2, s.~1-13. ISSN~2075-4450. Dostupné z: https://dx.doi.org/10.3390/insects13020193.

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