Direct and Indirect Antioxidant Effects of Selected Plant
Phenolics in Cell-Based Assays

J 2021

Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays

TREML, Jakub, P. VEČEŘOVÁ, Petra HERCZOGOVÁ a Karel ŠMEJKAL

Základní údaje

Originální název

Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays

Autoři

TREML, Jakub (203 Česká republika, garant, domácí), P. VEČEŘOVÁ (203 Česká republika), Petra HERCZOGOVÁ (703 Slovensko, domácí) a Karel ŠMEJKAL (203 Česká republika, domácí)

Vydání

Molecules, Basel, MDPI, 2021, 1420-3049

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.927

Kód RIV

RIV/00216224:14160/21:00124216

Organizační jednotka

Farmaceutická fakulta

DOI

http://dx.doi.org/10.3390/molecules26092534

UT WoS

000650681600001

Klíčová slova anglicky

antioxidants; CAA; catalase; glucose toxicity; plant phenolics; superoxide dismutase; Nrf2-ARE

Štítky

rivok, ÚMF, ÚPL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 2. 2022 18:13, JUDr. Sabina Krejčiříková

Anotace

V originále

Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. Conclusions: Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays.

Citovat

TREML, Jakub, P. VEČEŘOVÁ, Petra HERCZOGOVÁ a Karel ŠMEJKAL. Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays. Molecules. Basel: MDPI, 2021, roč. 26, č. 9, s. 1-15. ISSN 1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26092534.

@article{1835161,
   author = {Treml, Jakub and Večeřová, P. and Herczogová, Petra and Šmejkal, Karel},
   article_location = {Basel},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/molecules26092534},
   keywords = {antioxidants; CAA; catalase; glucose toxicity; plant phenolics; superoxide dismutase; Nrf2-ARE},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays},
   url = {https://www.mdpi.com/1420-3049/26/9/2534/htm#},
   volume = {26},
   year = {2021}
}

TY  - JOUR
ID  - 1835161
AU  - Treml, Jakub - Večeřová, P. - Herczogová, Petra - Šmejkal, Karel
PY  - 2021
TI  - Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays
JF  - Molecules
VL  - 26
IS  - 9
SP  - 1-15
EP  - 1-15
PB  - MDPI
SN  - 14203049
KW  - antioxidants
KW  - CAA
KW  - catalase
KW  - glucose toxicity
KW  - plant phenolics
KW  - superoxide dismutase
KW  - Nrf2-ARE
UR  - https://www.mdpi.com/1420-3049/26/9/2534/htm#
N2  - Background: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. Methods: The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. Results: Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. Conclusions: Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays.
ER  -

TREML, Jakub, P. VEČEŘOVÁ, Petra HERCZOGOVÁ a Karel ŠMEJKAL. Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays. \textit{Molecules}. Basel: MDPI, 2021, roč.~26, č.~9, s.~1-15. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26092534.

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