Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two
Variants of nimB Resistance Gene from Bacteroides fragilis, and
Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera
Isolated from Chicken Caeca

J 2022

Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca

KRÁLOVÁ, Stanislava, Lenka DAVIDOVA-GERZOVA, Adam VALCEK, Matěj BEZDÍČEK, Ivan RYCHLÍK et. al.

Základní údaje

Originální název

Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca

Autoři

KRÁLOVÁ, Stanislava (703 Slovensko, garant, domácí), Lenka DAVIDOVA-GERZOVA, Adam VALCEK, Matěj BEZDÍČEK (203 Česká republika, domácí), Ivan RYCHLÍK (203 Česká republika), Veronika REZACOVA a Alois ČÍŽEK (203 Česká republika)

Vydání

Microbiology Spectrum, American Society for Microbiology, 2022, 2165-0497

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700

Kód RIV

RIV/00216224:14310/22:00125419

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1128/spectrum.01954-21

UT WoS

000766015800155

Klíčová slova anglicky

phylogenomics; polyphasic taxonomy; Paraphocaeicola brunensis gen. nov.; sp. nov.; Caecibacteroides pullorum gen. nov.; sp. nov.; Bacteroidaceae; metronidazole resistance; nimB gene

Štítky

14110212, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 12. 2022 13:04, Mgr. Marie Šípková, DiS.

Anotace

V originále

Three difficult-to-cultivate, strictly anaerobic strains, AN20T, AN421T, and AN502, were analyzed within a project studying possible probiotics for newly hatched chickens. Phylogenetic analyses showed that strains AN20T, AN421T, and AN502 formed two well-separated phylogenetic lineages in all phylogenetic and phylogenomic trees comprising members of the family Bacteroidaceae. Comparison to reference genomes of type species Bacteroides fragilis NCTC 9343T, Phocaeicola abscessus CCUG 55929T, and Capsularis zoogleoformans ATCC 33285T showed low relatedness based on the calculated genome-to-genome distance and orthologous average nucleotide identity. Analysis of fatty acid profiles showed iso-C15:0, anteiso-C15:0, C16:0, C18:1 ω9c, and iso-C17:0 3OH as the major fatty acids for all three strains and additionally C16:0 3OH for AN421T and AN502. A specific combination of respiratory quinones different from related taxa was found in analyzed strains, MK-5 plus MK-11 in strain AN20T and MK-5 plus MK-10 in strains AN421T and AN502. Strains AN421T and AN502 harbor complete CRISPR loci with CRISPR array, type II-C, accompanied by a set of cas genes (cas9, cas1, and cas2) in close proximity. Interestingly, strain AN20T was found to harbor two copies of nimB gene with >95% similarity to nimB of B. fragilis, suggesting a horizontal gene transfer between these taxa. In summary, three isolates characterized in this study represent two novel species, which we proposed to be classified in two novel genera of the family Bacteroidaceae, for which the names Paraphocaeicola brunensis sp. nov. (AN20T = CCM 9041T = DSM 111154T) and Caecibacteroides pullorum sp. nov. (AN421T= CCM 9040T = DSM 111155T) are proposed.

Citovat

KRÁLOVÁ, Stanislava, Lenka DAVIDOVA-GERZOVA, Adam VALCEK, Matěj BEZDÍČEK, Ivan RYCHLÍK, Veronika REZACOVA a Alois ČÍŽEK. Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca. Microbiology Spectrum. American Society for Microbiology, 2022, roč. 10, č. 1, s. 1-21. ISSN 2165-0497. Dostupné z: https://dx.doi.org/10.1128/spectrum.01954-21.

@article{1835963,
   author = {Králová, Stanislava and DavidovaandGerzova, Lenka and Valcek, Adam and Bezdíček, Matěj and Rychlík, Ivan and Rezacova, Veronika and Čížek, Alois},
   article_number = {1},
   doi = {http://dx.doi.org/10.1128/spectrum.01954-21},
   keywords = {phylogenomics; polyphasic taxonomy; Paraphocaeicola brunensis gen. nov.; sp. nov.; Caecibacteroides pullorum gen. nov.; sp. nov.; Bacteroidaceae; metronidazole resistance; nimB gene},
   language = {eng},
   issn = {2165-0497},
   journal = {Microbiology Spectrum},
   title = {Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca},
   url = {https://journals.asm.org/doi/10.1128/spectrum.01954-21},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 1835963
AU  - Králová, Stanislava - Davidova-Gerzova, Lenka - Valcek, Adam - Bezdíček, Matěj - Rychlík, Ivan - Rezacova, Veronika - Čížek, Alois
PY  - 2022
TI  - Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca
JF  - Microbiology Spectrum
VL  - 10
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - American Society for Microbiology
SN  - 21650497
KW  - phylogenomics
KW  - polyphasic taxonomy
KW  - Paraphocaeicola brunensis gen. nov.
KW  - sp. nov.
KW  - Caecibacteroides pullorum gen. nov.
KW  - sp. nov.
KW  - Bacteroidaceae
KW  - metronidazole resistance
KW  - nimB gene
UR  - https://journals.asm.org/doi/10.1128/spectrum.01954-21
N2  - Three difficult-to-cultivate, strictly anaerobic strains, AN20T, AN421T, and AN502, were analyzed within a project studying possible probiotics for newly hatched chickens. Phylogenetic analyses showed that strains AN20T, AN421T, and AN502 formed two well-separated phylogenetic lineages in all phylogenetic and phylogenomic trees comprising members of the family Bacteroidaceae. Comparison to reference genomes of type species Bacteroides fragilis NCTC 9343T, Phocaeicola abscessus CCUG 55929T, and Capsularis zoogleoformans ATCC 33285T showed low relatedness based on the calculated genome-to-genome distance and orthologous average nucleotide identity. Analysis of fatty acid profiles showed iso-C15:0, anteiso-C15:0, C16:0, C18:1 ω9c, and iso-C17:0 3OH as the major fatty acids for all three strains and additionally C16:0 3OH for AN421T and AN502. A specific combination of respiratory quinones different from related taxa was found in analyzed strains, MK-5 plus MK-11 in strain AN20T and MK-5 plus MK-10 in strains AN421T and AN502. Strains AN421T and AN502 harbor complete CRISPR loci with CRISPR array, type II-C, accompanied by a set of cas genes (cas9, cas1, and cas2) in close proximity. Interestingly, strain AN20T was found to harbor two copies of nimB gene with >95% similarity to nimB of B. fragilis, suggesting a horizontal gene transfer between these taxa. In summary, three isolates characterized in this study represent two novel species, which we proposed to be classified in two novel genera of the family Bacteroidaceae, for which the names Paraphocaeicola brunensis sp. nov. (AN20T = CCM 9041T = DSM 111154T) and Caecibacteroides pullorum sp. nov. (AN421T= CCM 9040T = DSM 111155T) are proposed.
ER  -

KRÁLOVÁ, Stanislava, Lenka DAVIDOVA-GERZOVA, Adam VALCEK, Matěj BEZDÍČEK, Ivan RYCHLÍK, Veronika REZACOVA a Alois ČÍŽEK. Paraphocaeicola brunensis gen. nov., sp. nov., Carrying Two Variants of nimB Resistance Gene from Bacteroides fragilis, and Caecibacteroides pullorum gen. nov., sp. nov., Two Novel Genera Isolated from Chicken Caeca. \textit{Microbiology Spectrum}. American Society for Microbiology, 2022, roč.~10, č.~1, s.~1-21. ISSN~2165-0497. Dostupné z: https://dx.doi.org/10.1128/spectrum.01954-21.

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