Split-root systems: detailed methodology, alternative
applications, and implications at leaf proteome level

J 2021

Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

SAIZ-FERNANDEZ, I., M. CERNY, Jan SKALÁK a B. BRZOBOHATY

Základní údaje

Originální název

Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

Autoři

SAIZ-FERNANDEZ, I., M. CERNY, Jan SKALÁK (203 Česká republika, garant, domácí) a B. BRZOBOHATY

Vydání

PLANT METHODS, LONDON, BIOMED CENTRAL LTD, 2021, 1746-4811

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.827

Kód RIV

RIV/00216224:14740/21:00124248

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1186/s13007-020-00706-1

UT WoS

000606465100001

Klíčová slova anglicky

Arabidopsis thaliana; Drought stress; Proteomics; Phytohormones; Split-root systems

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 2. 2022 15:44, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Background: Split-root systems (SRS) have many applications in plant sciences, but their implementation, depending on the experimental design, can be difficult and time-consuming. Additionally, the system is not exempt from limitations, since the time required for the establishment of the SRS imposes a limit to how early in plant development experiments can be performed. Here, we optimized and explained in detail a method for establishing a SRS in young Arabidopsis thaliana seedlings, both in vitro and in soil. Results: We found that the partial de-rooting minimized the recovery time compared to total de-rooting, thus allowing the establishment of the split-root system in younger plants. Analysis of changes in the Arabidopsis leaf proteome following the de-rooting procedure highlighted the distinct metabolic alterations that totally and partially de-rooted plants undergo during the healing process. This system was also validated for its use in drought experiments, as it offers a way to apply water-soluble compounds to plants subjected to drought stress. By growing plants in a split-root system with both halves being water-deprived, it is possible to apply the required compound to one half of the root system, which can be cut from the main plant once the compound has been absorbed, thus minimizing rehydration and maintaining drought conditions. Conclusions: Partial de-rooting is the suggested method for obtaining split-root systems in small plants like Arabidopsis thaliana, as growth parameters, survival rate, and proteomic analysis suggest that is a less stressful procedure than total de-rooting, leading to a final rosette area much closer to that of uncut plants. Additionally, we provide evidence that split root-systems can be used in drought experiments where water-soluble compounds are applied with minimal effects of rehydration.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

SAIZ-FERNANDEZ, I., M. CERNY, Jan SKALÁK a B. BRZOBOHATY. Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level. PLANT METHODS. LONDON: BIOMED CENTRAL LTD, 2021, roč. 17, č. 1, s. „7“, 19 s. ISSN 1746-4811. Dostupné z: https://dx.doi.org/10.1186/s13007-020-00706-1.

@article{1836384,
   author = {SaizandFernandez, I. and Cerny, M. and Skalák, Jan and Brzobohaty, B.},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s13007-020-00706-1},
   keywords = {Arabidopsis thaliana; Drought stress; Proteomics; Phytohormones; Split-root systems},
   language = {eng},
   issn = {1746-4811},
   journal = {PLANT METHODS},
   title = {Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level},
   url = {https://plantmethods.biomedcentral.com/articles/10.1186/s13007-020-00706-1},
   volume = {17},
   year = {2021}
}

TY  - JOUR
ID  - 1836384
AU  - Saiz-Fernandez, I. - Cerny, M. - Skalák, Jan - Brzobohaty, B.
PY  - 2021
TI  - Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level
JF  - PLANT METHODS
VL  - 17
IS  - 1
SP  - „7“
EP  - „7“
PB  - BIOMED CENTRAL LTD
SN  - 17464811
KW  - Arabidopsis thaliana
KW  - Drought stress
KW  - Proteomics
KW  - Phytohormones
KW  - Split-root systems
UR  - https://plantmethods.biomedcentral.com/articles/10.1186/s13007-020-00706-1
N2  - Background: Split-root systems (SRS) have many applications in plant sciences, but their implementation, depending on the experimental design, can be difficult and time-consuming. Additionally, the system is not exempt from limitations, since the time required for the establishment of the SRS imposes a limit to how early in plant development experiments can be performed. Here, we optimized and explained in detail a method for establishing a SRS in young Arabidopsis thaliana seedlings, both in vitro and in soil. Results: We found that the partial de-rooting minimized the recovery time compared to total de-rooting, thus allowing the establishment of the split-root system in younger plants. Analysis of changes in the Arabidopsis leaf proteome following the de-rooting procedure highlighted the distinct metabolic alterations that totally and partially de-rooted plants undergo during the healing process. This system was also validated for its use in drought experiments, as it offers a way to apply water-soluble compounds to plants subjected to drought stress. By growing plants in a split-root system with both halves being water-deprived, it is possible to apply the required compound to one half of the root system, which can be cut from the main plant once the compound has been absorbed, thus minimizing rehydration and maintaining drought conditions. Conclusions: Partial de-rooting is the suggested method for obtaining split-root systems in small plants like Arabidopsis thaliana, as growth parameters, survival rate, and proteomic analysis suggest that is a less stressful procedure than total de-rooting, leading to a final rosette area much closer to that of uncut plants. Additionally, we provide evidence that split root-systems can be used in drought experiments where water-soluble compounds are applied with minimal effects of rehydration.
ER  -

SAIZ-FERNANDEZ, I., M. CERNY, Jan SKALÁK a B. BRZOBOHATY. Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level. \textit{PLANT METHODS}. LONDON: BIOMED CENTRAL LTD, 2021, roč.~17, č.~1, s.~„7“, 19 s. ISSN~1746-4811. Dostupné z: https://dx.doi.org/10.1186/s13007-020-00706-1.

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