Sheep as a Potential Model of Intradiscal Infection by the
Bacterium Cutibacterium acnes

J 2021

Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes

COSCIA, E.C., N.S. ABUTALEB, B. HOSTETTER, M.N. SELEEM, G.J. BREUR et. al.

Základní údaje

Originální název

Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes

Autoři

COSCIA, E.C., N.S. ABUTALEB, B. HOSTETTER, M.N. SELEEM, G.J. BREUR, R.R. MCCAIN, C.J. CRAIN, Ondřej SLABÝ (203 Česká republika, garant, domácí), M.N. CAPOOR, A. MCDOWELL, externista FAHAD AHMED (840 Spojené státy), V. VIJAYANPILLAI, S.K. NARAYANAN a M.F. COSCIA

Vydání

Polish Journal of Veterinary Sciences, Olsztyn, Polish Academy of Sciences Committee of Veterinary Sciences, University of Warmia and Mazury in Olsztyn, 2021, 2306-7381

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.518

Kód RIV

RIV/00216224:14740/21:00124251

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/vetsci8030048

UT WoS

000634239500001

Klíčová slova anglicky

Cutibacterium acnes; sheep model; spinal intervertebral discs; percutaneous injections; bacterial discitis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 5. 2022 13:39, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The anaerobic bacterium Cutibacterium acnes has been increasingly linked to the development of degenerative disc disease (DDD), although causality is yet to be conclusively proven. To better study how this organism could contribute to the aetiology of DDD, improved animal models that are more reflective of human disc anatomy, biology and mechanical properties are required. Against this background, our proof-of concept study aimed to be the first demonstration that C. acnes could be safely administered percutaneously into sheep intervertebral discs (IVDs) for in vivo study. Following our protocol, two sheep were successfully injected with a strain of C. acnes (8.3 x 10(6) CFU/disc) previously recovered from a human degenerative disc. No adverse reactions were noted, and at one-month post inoculation all triplicate infected discs in our first animal grew C. acnes, albeit at a reduced load (5.12 x 10(4) to 6.67 x 10(4) CFU/disc). At six months, no growth was detected in discs from our second animal indicating bacterial clearance. This pilot study has demonstrated the feasibility of safe percutaneous injection of C. acnes into sheep IVDs under fluoroscopic guidance. The design of follow-up sheep studies to investigate the potential of C. acnes to drive pathological changes within infected discs should now be pursued.

Citovat

COSCIA, E.C., N.S. ABUTALEB, B. HOSTETTER, M.N. SELEEM, G.J. BREUR, R.R. MCCAIN, C.J. CRAIN, Ondřej SLABÝ, M.N. CAPOOR, A. MCDOWELL, externista FAHAD AHMED, V. VIJAYANPILLAI, S.K. NARAYANAN a M.F. COSCIA. Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes. Polish Journal of Veterinary Sciences. Olsztyn: Polish Academy of Sciences Committee of Veterinary Sciences, University of Warmia and Mazury in Olsztyn, 2021, roč. 8, č. 3, s. 48-56. ISSN 2306-7381. Dostupné z: https://dx.doi.org/10.3390/vetsci8030048.

@article{1836427,
   author = {Coscia, E.C. and Abutaleb, N.S. and Hostetter, B. and Seleem, M.N. and Breur, G.J. and McCain, R.R. and Crain, C.J. and Slabý, Ondřej and Capoor, M.N. and McDowell, A. and Fahad Ahmed, externista and Vijayanpillai, V. and Narayanan, S.K. and Coscia, M.F.},
   article_location = {Olsztyn},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/vetsci8030048},
   keywords = {Cutibacterium acnes; sheep model; spinal intervertebral discs; percutaneous injections; bacterial discitis},
   language = {eng},
   issn = {2306-7381},
   journal = {Polish Journal of Veterinary Sciences},
   title = {Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes},
   url = {https://www.mdpi.com/2306-7381/8/3/48},
   volume = {8},
   year = {2021}
}

TY  - JOUR
ID  - 1836427
AU  - Coscia, E.C. - Abutaleb, N.S. - Hostetter, B. - Seleem, M.N. - Breur, G.J. - McCain, R.R. - Crain, C.J. - Slabý, Ondřej - Capoor, M.N. - McDowell, A. - Fahad Ahmed, externista - Vijayanpillai, V. - Narayanan, S.K. - Coscia, M.F.
PY  - 2021
TI  - Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes
JF  - Polish Journal of Veterinary Sciences
VL  - 8
IS  - 3
SP  - 48
EP  - 48
PB  - Polish Academy of Sciences Committee of Veterinary Sciences, University of Warmia and Mazury in Olsztyn
SN  - 23067381
KW  - Cutibacterium acnes
KW  - sheep model
KW  - spinal intervertebral discs
KW  - percutaneous injections
KW  - bacterial discitis
UR  - https://www.mdpi.com/2306-7381/8/3/48
N2  - The anaerobic bacterium Cutibacterium acnes has been increasingly linked to the development of degenerative disc disease (DDD), although causality is yet to be conclusively proven. To better study how this organism could contribute to the aetiology of DDD, improved animal models that are more reflective of human disc anatomy, biology and mechanical properties are required. Against this background, our proof-of concept study aimed to be the first demonstration that C. acnes could be safely administered percutaneously into sheep intervertebral discs (IVDs) for in vivo study. Following our protocol, two sheep were successfully injected with a strain of C. acnes (8.3 x 10(6) CFU/disc) previously recovered from a human degenerative disc. No adverse reactions were noted, and at one-month post inoculation all triplicate infected discs in our first animal grew C. acnes, albeit at a reduced load (5.12 x 10(4) to 6.67 x 10(4) CFU/disc). At six months, no growth was detected in discs from our second animal indicating bacterial clearance. This pilot study has demonstrated the feasibility of safe percutaneous injection of C. acnes into sheep IVDs under fluoroscopic guidance. The design of follow-up sheep studies to investigate the potential of C. acnes to drive pathological changes within infected discs should now be pursued.
ER  -

COSCIA, E.C., N.S. ABUTALEB, B. HOSTETTER, M.N. SELEEM, G.J. BREUR, R.R. MCCAIN, C.J. CRAIN, Ondřej SLABÝ, M.N. CAPOOR, A. MCDOWELL, externista FAHAD AHMED, V. VIJAYANPILLAI, S.K. NARAYANAN a M.F. COSCIA. Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes. \textit{Polish Journal of Veterinary Sciences}. Olsztyn: Polish Academy of Sciences Committee of Veterinary Sciences, University of Warmia and Mazury in Olsztyn, 2021, roč.~8, č.~3, s.~48-56. ISSN~2306-7381. Dostupné z: https://dx.doi.org/10.3390/vetsci8030048.

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