| MARINOVIC, S., A. SKRTIC, Tina CATELA IVKOVIĆ, M. POLJAK a S. KAPITANOVIC. Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma. HUMAN CELL. TOKYO: SPRINGER JAPAN KK, 2021, roč. 34, č. 5, s. 1455-1465. ISSN 0914-7470. Dostupné z: https://dx.doi.org/10.1007/s13577-021-00576-2. |
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@article{1836441,
author = {Marinovic, S. and Skrtic, A. and Catela Ivković, Tina and Poljak, M. and Kapitanovic, S.},
article_location = {TOKYO},
article_number = {5},
doi = {http://dx.doi.org/10.1007/s13577-021-00576-2},
keywords = {Colon adenocarcinoma; Immunohistochemistry; KRAS; Let-7a; miR-544a},
language = {eng},
issn = {0914-7470},
journal = {HUMAN CELL},
title = {Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma},
url = {https://link.springer.com/article/10.1007/s13577-021-00576-2},
volume = {34},
year = {2021}
}
TY - JOUR
ID - 1836441
AU - Marinovic, S. - Skrtic, A. - Catela Ivković, Tina - Poljak, M. - Kapitanovic, S.
PY - 2021
TI - Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma
JF - HUMAN CELL
VL - 34
IS - 5
SP - 1455-1465
EP - 1455-1465
PB - SPRINGER JAPAN KK
SN - 09147470
KW - Colon adenocarcinoma
KW - Immunohistochemistry
KW - KRAS
KW - Let-7a
KW - miR-544a
UR - https://link.springer.com/article/10.1007/s13577-021-00576-2
N2 - Colorectal carcinoma (CRC) results from the accumulation of genetic mutations and alterations in signaling pathways. KRAS is mutated in 40% of CRC cases and is involved in increased tumor cells proliferation and survival. Although KRAS mutations are a dominant event in CRC tumorigenesis, increased wild-type KRAS expression has a similar effect on accelerated tumor growth. In this study, we investigated the KRAS status in correlation with clinicopathological features in sporadic CRC and more importantly the role of let-7a-5p and miR-544a-3p in the regulation of wild-type KRAS protein expression in the tumor center (T1) and invasive tumor front (T2). Analysis showed that 39.1% of tumor samples had KRAS mutations. In wild-type KRAS tumors, 62.0% were positive for KRAS protein expression and there was a higher percentage of KRAS-positive tumor cells and a higher intensity of immunohistochemical reaction in T2 than in T1 samples. This could not be attributed to differences in KRAS mRNA levels, suggesting regulation via miR-544a-3p expression which was significantly decreased in T2 samples. Furthermore, we demonstrated that tumor samples carrying the KRAS-LCS6 variant allele had significantly higher protein expression of the wild-type KRAS. Our results suggest the role of the KRAS-LCS6 polymorphism and miR-544a-3p expression in the regulation of wild-type KRAS protein expression in sporadic CRC.
ER -
MARINOVIC, S., A. SKRTIC, Tina CATELA IVKOVI$\backslash$'C, M. POLJAK a S. KAPITANOVIC. Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma. \textit{HUMAN CELL}. TOKYO: SPRINGER JAPAN KK, 2021, roč.~34, č.~5, s.~1455-1465. ISSN~0914-7470. Dostupné z: https://dx.doi.org/10.1007/s13577-021-00576-2.
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