Complex Alterations of Fatty Acid Metabolism and
Phospholipidome Uncovered in Isolated Colon Cancer Epithelial
Cells

J 2021

Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells

HOFMANOVÁ, Jiřina, Josef SLAVIK, Miroslav CIGANEK, Petra OVESNÁ, Zuzana TYLICHOVA et. al.

Základní údaje

Originální název

Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells

Autoři

HOFMANOVÁ, Jiřina (203 Česká republika, domácí), Josef SLAVIK (203 Česká republika), Miroslav CIGANEK (203 Česká republika), Petra OVESNÁ (203 Česká republika, domácí), Zuzana TYLICHOVA (203 Česká republika), Martina KARASOVÁ (203 Česká republika, domácí), Ondrej ZAPLETAL (203 Česká republika), Nicol STRAKOVA (203 Česká republika), Jirina PROCHAZKOVA (203 Česká republika), Jan BOUCHAL (203 Česká republika), Zdenek KOLAR (203 Česká republika), Jiri EHRMANN (203 Česká republika), Monika LEVKOVA (203 Česká republika), Zlatka HUSKOVA (203 Česká republika), Pavel SKALICKY (203 Česká republika), Alois KOZUBÍK (203 Česká republika, domácí), Miroslav MACHALA (203 Česká republika) a Jan VONDRÁČEK (203 Česká republika, garant)

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.208

Kód RIV

RIV/00216224:14310/21:00124278

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/ijms22136650

UT WoS

000671202800001

Klíčová slova anglicky

phospholipids; lysophospholipids; fatty acid synthesis; desaturation; colorectal carcinoma; EpCAM; epithelial cells; lipidomics

Štítky

14119612, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 2. 2022 09:02, Mgr. Marie Šípková, DiS.

Anotace

V originále

The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.

Citovat

HOFMANOVÁ, Jiřina, Josef SLAVIK, Miroslav CIGANEK, Petra OVESNÁ, Zuzana TYLICHOVA, Martina KARASOVÁ, Ondrej ZAPLETAL, Nicol STRAKOVA, Jirina PROCHAZKOVA, Jan BOUCHAL, Zdenek KOLAR, Jiri EHRMANN, Monika LEVKOVA, Zlatka HUSKOVA, Pavel SKALICKY, Alois KOZUBÍK, Miroslav MACHALA a Jan VONDRÁČEK. Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2021, roč. 22, č. 13, s. 1-16. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms22136650.

@article{1836814,
   author = {Hofmanová, Jiřina and Slavik, Josef and Ciganek, Miroslav and Ovesná, Petra and Tylichova, Zuzana and Karasová, Martina and Zapletal, Ondrej and Strakova, Nicol and Prochazkova, Jirina and Bouchal, Jan and Kolar, Zdenek and Ehrmann, Jiri and Levkova, Monika and Huskova, Zlatka and Skalicky, Pavel and Kozubík, Alois and Machala, Miroslav and Vondráček, Jan},
   article_location = {Basel},
   article_number = {13},
   doi = {http://dx.doi.org/10.3390/ijms22136650},
   keywords = {phospholipids; lysophospholipids; fatty acid synthesis; desaturation; colorectal carcinoma; EpCAM; epithelial cells; lipidomics},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells},
   url = {https://www.mdpi.com/1422-0067/22/13/6650},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1836814
AU  - Hofmanová, Jiřina - Slavik, Josef - Ciganek, Miroslav - Ovesná, Petra - Tylichova, Zuzana - Karasová, Martina - Zapletal, Ondrej - Strakova, Nicol - Prochazkova, Jirina - Bouchal, Jan - Kolar, Zdenek - Ehrmann, Jiri - Levkova, Monika - Huskova, Zlatka - Skalicky, Pavel - Kozubík, Alois - Machala, Miroslav - Vondráček, Jan
PY  - 2021
TI  - Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 13
SP  - 1-16
EP  - 1-16
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - phospholipids
KW  - lysophospholipids
KW  - fatty acid synthesis
KW  - desaturation
KW  - colorectal carcinoma
KW  - EpCAM
KW  - epithelial cells
KW  - lipidomics
UR  - https://www.mdpi.com/1422-0067/22/13/6650
N2  - The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.
ER  -

HOFMANOVÁ, Jiřina, Josef SLAVIK, Miroslav CIGANEK, Petra OVESNÁ, Zuzana TYLICHOVA, Martina KARASOVÁ, Ondrej ZAPLETAL, Nicol STRAKOVA, Jirina PROCHAZKOVA, Jan BOUCHAL, Zdenek KOLAR, Jiri EHRMANN, Monika LEVKOVA, Zlatka HUSKOVA, Pavel SKALICKY, Alois KOZUBÍK, Miroslav MACHALA a Jan VONDRÁČEK. Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2021, roč.~22, č.~13, s.~1-16. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms22136650.

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