G2/M-checkpoint activation in fasciata1 rescues an aberrant
S-phase checkpoint but causes genome instability

J 2021

G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability

EEKHOUT, T., Martina DVOŘÁČKOVÁ, J.A.P. GARCIA, Martina NEŠPOR DADEJOVÁ, P. KALHORZADEH et. al.

Základní údaje

Originální název

G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability

Autoři

EEKHOUT, T., Martina DVOŘÁČKOVÁ (203 Česká republika, domácí), J.A.P. GARCIA, Martina NEŠPOR DADEJOVÁ (203 Česká republika, domácí), P. KALHORZADEH, H. VAN DEN DAELE, I. VERCAUTEREN, Jiří FAJKUS (203 Česká republika, garant, domácí) a L. DE VEYLDER

Vydání

Plant Physiology, ROCKVILLE, American Society of Plant Physiologists, 2021, 0032-0889

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.005

Kód RIV

RIV/00216224:14740/21:00119672

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/plphys/kiab201

UT WoS

000703922000018

Klíčová slova anglicky

DNA-DAMAGE RESPONSEDEPENDENT KINASE INHIBITORSASSEMBLY FACTOR-IARABIDOPSIS-THALIANAHOMOLOGOUS RECOMBINATION45S RDNACAF-1ATRREPLICATIONDIFFERENTIATION

Štítky

CF PLANT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2022 10:57, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The WEE1 and ATM AND RAD3-RELATED (ATR) kinases are important regulators of the plant intra-S-phase checkpoint; consequently, WEE1(KO) and ATR(KO) roots are hypersensitive to replication-inhibitory drugs. Here, we report on a loss-of-function mutant allele of the FASCIATA1 (FAS1) subunit of the chromatin assembly factor 1 (CAF-1) complex that suppresses the phenotype of WEE1- or ATR-deficient Arabidopsis (Arabidopsis thaliana) plants. We demonstrate that lack of FAS1 activity results in the activation of an ATAXIA TELANGIECTASIA MUTATED (ATM)- and SUPPRESSOR OF GAMMA-RESPONSE 1 (SOG1)-mediated G2/M-arrest that renders the ATR and WEE1 checkpoint regulators redundant. This ATM activation accounts for the telomere erosion and loss of ribosomal DNA that are described for fast plants. Knocking out SOG1 in the fast weel background restores replication stress sensitivity, demonstrating that SOG1 is an important secondary checkpoint regulator in plants that fail to activate the intra-S-phase checkpoint.

Návaznosti

GJ19-11880Y, projekt VaV

Název: Chromatinové prostředí indukované genotoxickými látkami

Investor: Grantová agentura ČR, Chromatinové prostředí indukované genotoxickými látkami

GX20-01331X, projekt VaV

Název: Biogeneze a evoluce telomerázy u rostlin

Investor: Grantová agentura ČR, Biogenesis and evolution of telomerase in plants

LTC18048, projekt VaV

Název: Prostorové uspořádání významných jaderných domén a jejich proteinových složek

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Prostorové uspořádání významných jaderných domén a jejich proteinových složek, INTER-COST

LTC20003, projekt VaV

Název: Dynamics of telomeres and rDNA loci in plant cell nuclei

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Dynamics of telomeres and rDNA loci in plant cell nuclei, INTER-COST

Citovat

EEKHOUT, T., Martina DVOŘÁČKOVÁ, J.A.P. GARCIA, Martina NEŠPOR DADEJOVÁ, P. KALHORZADEH, H. VAN DEN DAELE, I. VERCAUTEREN, Jiří FAJKUS a L. DE VEYLDER. G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability. Plant Physiology. ROCKVILLE: American Society of Plant Physiologists, 2021, roč. 186, č. 4, s. 1893-1907. ISSN 0032-0889. Dostupné z: https://dx.doi.org/10.1093/plphys/kiab201.

@article{1837260,
   author = {Eekhout, T. and Dvořáčková, Martina and Garcia, J.A.P. and Nešpor Dadejová, Martina and Kalhorzadeh, P. and Van den Daele, H. and Vercauteren, I. and Fajkus, Jiří and De Veylder, L.},
   article_location = {ROCKVILLE},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/plphys/kiab201},
   keywords = {DNA-DAMAGE RESPONSEDEPENDENT KINASE INHIBITORSASSEMBLY FACTOR-IARABIDOPSIS-THALIANAHOMOLOGOUS RECOMBINATION45S RDNACAF-1ATRREPLICATIONDIFFERENTIATION},
   language = {eng},
   issn = {0032-0889},
   journal = {Plant Physiology},
   title = {G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability},
   url = {https://academic.oup.com/plphys/article/186/4/1893/6270794},
   volume = {186},
   year = {2021}
}

TY  - JOUR
ID  - 1837260
AU  - Eekhout, T. - Dvořáčková, Martina - Garcia, J.A.P. - Nešpor Dadejová, Martina - Kalhorzadeh, P. - Van den Daele, H. - Vercauteren, I. - Fajkus, Jiří - De Veylder, L.
PY  - 2021
TI  - G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability
JF  - Plant Physiology
VL  - 186
IS  - 4
SP  - 1893-1907
EP  - 1893-1907
PB  - American Society of Plant Physiologists
SN  - 00320889
KW  - DNA-DAMAGE RESPONSEDEPENDENT KINASE INHIBITORSASSEMBLY FACTOR-IARABIDOPSIS-THALIANAHOMOLOGOUS RECOMBINATION45S RDNACAF-1ATRREPLICATIONDIFFERENTIATION
UR  - https://academic.oup.com/plphys/article/186/4/1893/6270794
N2  - The WEE1 and ATM AND RAD3-RELATED (ATR) kinases are important regulators of the plant intra-S-phase checkpoint; consequently, WEE1(KO) and ATR(KO) roots are hypersensitive to replication-inhibitory drugs. Here, we report on a loss-of-function mutant allele of the FASCIATA1 (FAS1) subunit of the chromatin assembly factor 1 (CAF-1) complex that suppresses the phenotype of WEE1- or ATR-deficient Arabidopsis (Arabidopsis thaliana) plants. We demonstrate that lack of FAS1 activity results in the activation of an ATAXIA TELANGIECTASIA MUTATED (ATM)- and SUPPRESSOR OF GAMMA-RESPONSE 1 (SOG1)-mediated G2/M-arrest that renders the ATR and WEE1 checkpoint regulators redundant. This ATM activation accounts for the telomere erosion and loss of ribosomal DNA that are described for fast plants. Knocking out SOG1 in the fast weel background restores replication stress sensitivity, demonstrating that SOG1 is an important secondary checkpoint regulator in plants that fail to activate the intra-S-phase checkpoint.
ER  -

EEKHOUT, T., Martina DVOŘÁČKOVÁ, J.A.P. GARCIA, Martina NEŠPOR DADEJOVÁ, P. KALHORZADEH, H. VAN DEN DAELE, I. VERCAUTEREN, Jiří FAJKUS a L. DE VEYLDER. G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability. \textit{Plant Physiology}. ROCKVILLE: American Society of Plant Physiologists, 2021, roč.~186, č.~4, s.~1893-1907. ISSN~0032-0889. Dostupné z: https://dx.doi.org/10.1093/plphys/kiab201.

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