2022
B1-type cyclins control microtubule organization during cell division in Arabidopsis
MOTTA, M.R., X. ZHAO, M. PASTUGLIA, K. BELCRAM, F. ROODBARKELARI et. al.Základní údaje
Originální název
B1-type cyclins control microtubule organization during cell division in Arabidopsis
Autoři
MOTTA, M.R., X. ZHAO, M. PASTUGLIA, K. BELCRAM, F. ROODBARKELARI, M. KOMAKI, H. HARASHIMA, S. KOMAKI, M. KUMAR, P. BULANKOVA, M. HEESE, Karel ŘÍHA (203 Česká republika, garant, domácí), D. BOUCHEZ a A. SCHNITTGER
Vydání
EMBO reports, Hoboken, Wiley-Blackwell, 2022, 1469-221X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10611 Plant sciences, botany
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 7.700
Kód RIV
RIV/00216224:14740/22:00125443
Organizační jednotka
Středoevropský technologický institut
UT WoS
000728233300001
Klíčová slova anglicky
CDK; CYCB1; endosperm; microtubule nucleation; mitosis
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 6. 2. 2023 19:25, Mgr. Pavla Foltynová, Ph.D.
Anotace
V originále
Flowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitotic cyclins in cell cycle control during Arabidopsis development. While we reveal CYCB1;5 to be a pseudogene, the remaining four members were found to be expressed in dividing cells. Mutant analyses showed a complex pattern of overlapping, development-specific requirements of B1-type cyclins with CYCB1;2 playing a central role. The double mutant cycb1;1 cycb1;2 is severely compromised in growth, yet viable beyond the seedling stage, hence representing a unique opportunity to study the function of B1-type cyclin activity at the organismic level. Immunolocalization of microtubules in cycb1;1 cycb1;2 and treating mutants with the microtubule drug oryzalin revealed a key role of B1-type cyclins in orchestrating mitotic microtubule networks. Subsequently, we identified the GAMMA-TUBULIN COMPLEX PROTEIN 3-INTERACTING PROTEIN 1 (GIP1/MOZART) as an in vitro substrate of B1-type cyclin complexes and further genetic analyses support a potential role in the regulation of GIP1 by CYCB1s.
Návaznosti
EF15_003/0000479, projekt VaV |
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