A hypomorphic allele of telomerase uncovers the minimal
functional length of telomeres in Arabidopsis

J 2021

A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis

WATSON, J.M., J. TRIEB, M. TROESTL, K. RENFREW, Terezie MALÍK MANDÁKOVÁ et. al.

Základní údaje

Originální název

A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis

Autoři

WATSON, J.M., J. TRIEB, M. TROESTL, K. RENFREW, Terezie MALÍK MANDÁKOVÁ (203 Česká republika, domácí), Jaroslav FULNEČEK (203 Česká republika, domácí), D.E. SHIPPEN a Karel ŘÍHA (203 Česká republika, garant, domácí)

Vydání

Genetics, BETHESDA (USA), GENETICS SOCIETY AMERICA, 2021, 0016-6731

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.402

Kód RIV

RIV/00216224:14740/21:00119673

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/genetics/iyab126

UT WoS

000708515000019

Klíčová slova anglicky

telomeres; telomerase; genome stability; subtelomere; t-loop

Štítky

CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 10. 2024 10:02, Ing. Martina Blahová

Anotace

V originále

Despite the essential requirement of telomeric DNA for genome stability, the length of telomere tracts between species substantially differs, raising the question of the minimal length of telomeric DNA necessary for proper function. Here, we address this question using a hypomorphic allele of the telomerase catalytic subunit, TERT. We show that although this construct partially restored telomerase activity to a tert mutant, telomeres continued to shorten over several generations, ultimately stabilizing at a bimodal size distribution. Telomeres on two chromosome arms were maintained at a length of 1 kb, while the remaining telomeres were maintained at 400 bp. The longest telomeres identified in this background were also significantly longer in wild-type populations, suggesting cis-acting elements on these arms either promote telomerase processivity or recruitment. Genetically disrupting telomerase processivity in this background resulted in immediate lethality. Thus, telomeres of 400 bp are both necessary and sufficient for Arabidopsis viability. As this length is the estimated minimal length for t-loop formation, our data suggest that telomeres long enough to form a t-loop constitute the minimal functional length.

Návaznosti

EF15_003/0000479, projekt VaV

Název: Regulace rostlinné meiózy

GA18-20134S, projekt VaV

Název: Organizace interfázních chromosomů a časné meiotické párování chromosomů u brukvovitých

Investor: Grantová agentura ČR, Organizace interfázních chromosomů a časné meiotické párování chromosomů u brukvovitých

GA19-21961S, projekt VaV

Název: Chemická modulace aktivity Ku komplexu a využití v editaci genomů.

Investor: Grantová agentura ČR, Chemická modulace aktivity Ku komplexu a využití v editaci genomů

1304130933, interní kód MU

Název: Blunt-ended telomeres: dissecting the novel mechanism of chromosome end protection

Investor: EMBO (European Molecular Biology Organization), Blunt-ended telomeres: dissecting the novel mechanism of chromosome end protection

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

WATSON, J.M., J. TRIEB, M. TROESTL, K. RENFREW, Terezie MALÍK MANDÁKOVÁ, Jaroslav FULNEČEK, D.E. SHIPPEN a Karel ŘÍHA. A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis. Genetics. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2021, roč. 219, č. 2, s. „iyab126“, 9 s. ISSN 0016-6731. Dostupné z: https://dx.doi.org/10.1093/genetics/iyab126.

@article{1837299,
   author = {Watson, J.M. and Trieb, J. and Troestl, M. and Renfrew, K. and Malík Mandáková, Terezie and Fulneček, Jaroslav and Shippen, D.E. and Říha, Karel},
   article_location = {BETHESDA (USA)},
   article_number = {2},
   doi = {http://dx.doi.org/10.1093/genetics/iyab126},
   keywords = {telomeres; telomerase; genome stability; subtelomere; t-loop},
   language = {eng},
   issn = {0016-6731},
   journal = {Genetics},
   title = {A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis},
   url = {https://academic.oup.com/genetics/article-abstract/219/2/iyab126/6339584?redirectedFrom=fulltext},
   volume = {219},
   year = {2021}
}

TY  - JOUR
ID  - 1837299
AU  - Watson, J.M. - Trieb, J. - Troestl, M. - Renfrew, K. - Malík Mandáková, Terezie - Fulneček, Jaroslav - Shippen, D.E. - Říha, Karel
PY  - 2021
TI  - A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis
JF  - Genetics
VL  - 219
IS  - 2
SP  - „iyab126“
EP  - „iyab126“
PB  - GENETICS SOCIETY AMERICA
SN  - 00166731
KW  - telomeres
KW  - telomerase
KW  - genome stability
KW  - subtelomere
KW  - t-loop
UR  - https://academic.oup.com/genetics/article-abstract/219/2/iyab126/6339584?redirectedFrom=fulltext
N2  - Despite the essential requirement of telomeric DNA for genome stability, the length of telomere tracts between species substantially differs, raising the question of the minimal length of telomeric DNA necessary for proper function. Here, we address this question using a hypomorphic allele of the telomerase catalytic subunit, TERT. We show that although this construct partially restored telomerase activity to a tert mutant, telomeres continued to shorten over several generations, ultimately stabilizing at a bimodal size distribution. Telomeres on two chromosome arms were maintained at a length of 1 kb, while the remaining telomeres were maintained at 400 bp. The longest telomeres identified in this background were also significantly longer in wild-type populations, suggesting cis-acting elements on these arms either promote telomerase processivity or recruitment. Genetically disrupting telomerase processivity in this background resulted in immediate lethality. Thus, telomeres of 400 bp are both necessary and sufficient for Arabidopsis viability. As this length is the estimated minimal length for t-loop formation, our data suggest that telomeres long enough to form a t-loop constitute the minimal functional length.
ER  -

WATSON, J.M., J. TRIEB, M. TROESTL, K. RENFREW, Terezie MALÍK MANDÁKOVÁ, Jaroslav FULNEČEK, D.E. SHIPPEN a Karel ŘÍHA. A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis. \textit{Genetics}. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2021, roč.~219, č.~2, s.~„iyab126“, 9 s. ISSN~0016-6731. Dostupné z: https://dx.doi.org/10.1093/genetics/iyab126.

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