Rap1 regulates TIP60 function during fate transition between
two-cell-like and pluripotent states

J 2022

Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states

RAYMOND MARIO, Barry, Olivia SACCO, Amel MAMERI, Martin STOJASPAL, William KARTSONIS et. al.

Základní údaje

Originální název

Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states

Autoři

RAYMOND MARIO, Barry, Olivia SACCO, Amel MAMERI, Martin STOJASPAL (203 Česká republika, domácí), William KARTSONIS, Pooja SHAH, Pablo DE IOANNES, Ctirad HOFR (203 Česká republika, garant, domácí), Jacques CÔTÉ a Agnel SFEIR

Vydání

Genes & Development, Cold Spring Harbor Laboratory Press, 2022, 0890-9369

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.500

Kód RIV

RIV/00216224:14310/22:00125447

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1101/gad.349039.121

UT WoS

000821506300006

Klíčová slova anglicky

2C-like; EPC1; MERVL; RAP1; TIP60; ZSCAN4; telomere

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 3. 2023 10:29, Mgr. Martin Stojaspal, Ph.D.

Anotace

V originále

In mammals, the conserved telomere binding protein Rap1 serves a diverse set of nontelomeric functions, including activation of the NF-kB signaling pathway, maintenance of metabolic function in vivo, and transcriptional regulation. Here, we uncover the mechanism by which Rap1 modulates gene expression. Using a separation-of-function allele, we show that Rap1 transcriptional regulation is largely independent of TRF2-mediated binding to telomeres and does not involve direct binding to genomic loci. Instead, Rap1 interacts with the TIP60/p400 complex and modulates its histone acetyltransferase activity. Notably, we show that deletion of Rap1 in mouse embryonic stem cells increases the fraction of two-cell-like cells. Specifically, Rap1 enhances the repressive activity of Tip60/p400 across a subset of two-cell-stage genes, including Zscan4 and the endogenous retrovirus MERVL. Preferential upregulation of genes proximal to MERVL elements in Rap1-deficient settings implicates these endogenous retroviral elements in the derepression of proximal genes. Altogether, our study reveals an unprecedented link between Rap1 and the TIP60/p400 complex in the regulation of pluripotency.

Návaznosti

GA19-18226S, projekt VaV

Název: Kritické interakce neuronového transkripčního faktoru REST se stabilizátorem TRF2: biofyzikální implikace pro návrh léčiv glioblastomu

Investor: Grantová agentura ČR, Kritické interakce neuronového transkripčního faktoru REST se stabilizátorem TRF2: biofyzikální implikace pro návrh léčiv glioblastomu

LTAUSA19024, projekt VaV

Název: Role regulace transkripce při chemoterapeutické rezistenci glioblastomu: in vitro a in vivo validace cíle pro molekulární terapii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Role regulace transkripce při chemoterapeutické rezistenci glioblastomu: in vitro a in vivo validace cíle pro molekulární terapii, INTER-ACTION

Citovat

RAYMOND MARIO, Barry, Olivia SACCO, Amel MAMERI, Martin STOJASPAL, William KARTSONIS, Pooja SHAH, Pablo DE IOANNES, Ctirad HOFR, Jacques CÔTÉ a Agnel SFEIR. Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states. Genes & Development. Cold Spring Harbor Laboratory Press, 2022, roč. 36, 5-6, s. 313-330. ISSN 0890-9369. Dostupné z: https://dx.doi.org/10.1101/gad.349039.121.

@article{1837781,
   author = {Raymond Mario, Barry and Sacco, Olivia and Mameri, Amel and Stojaspal, Martin and Kartsonis, William and Shah, Pooja and De Ioannes, Pablo and Hofr, Ctirad and Côté, Jacques and Sfeir, Agnel},
   article_number = {5-6},
   doi = {http://dx.doi.org/10.1101/gad.349039.121},
   keywords = {2C-like; EPC1; MERVL; RAP1; TIP60; ZSCAN4; telomere},
   language = {eng},
   issn = {0890-9369},
   journal = {Genes & Development},
   title = {Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states},
   url = {http://genesdev.cshlp.org/content/early/2022/02/23/gad.349039.121.long},
   volume = {36},
   year = {2022}
}

TY  - JOUR
ID  - 1837781
AU  - Raymond Mario, Barry - Sacco, Olivia - Mameri, Amel - Stojaspal, Martin - Kartsonis, William - Shah, Pooja - De Ioannes, Pablo - Hofr, Ctirad - Côté, Jacques - Sfeir, Agnel
PY  - 2022
TI  - Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states
JF  - Genes & Development
VL  - 36
IS  - 5-6
SP  - 313-330
EP  - 313-330
PB  - Cold Spring Harbor Laboratory Press
SN  - 08909369
KW  - 2C-like
KW  - EPC1
KW  - MERVL
KW  - RAP1
KW  - TIP60
KW  - ZSCAN4
KW  - telomere
UR  - http://genesdev.cshlp.org/content/early/2022/02/23/gad.349039.121.long
N2  - In mammals, the conserved telomere binding protein Rap1 serves a diverse set of nontelomeric functions, including activation of the NF-kB signaling pathway, maintenance of metabolic function in vivo, and transcriptional regulation. Here, we uncover the mechanism by which Rap1 modulates gene expression. Using a separation-of-function allele, we show that Rap1 transcriptional regulation is largely independent of TRF2-mediated binding to telomeres and does not involve direct binding to genomic loci. Instead, Rap1 interacts with the TIP60/p400 complex and modulates its histone acetyltransferase activity. Notably, we show that deletion of Rap1 in mouse embryonic stem cells increases the fraction of two-cell-like cells. Specifically, Rap1 enhances the repressive activity of Tip60/p400 across a subset of two-cell-stage genes, including Zscan4 and the endogenous retrovirus MERVL. Preferential upregulation of genes proximal to MERVL elements in Rap1-deficient settings implicates these endogenous retroviral elements in the derepression of proximal genes. Altogether, our study reveals an unprecedented link between Rap1 and the TIP60/p400 complex in the regulation of pluripotency.
ER  -

RAYMOND MARIO, Barry, Olivia SACCO, Amel MAMERI, Martin STOJASPAL, William KARTSONIS, Pooja SHAH, Pablo DE IOANNES, Ctirad HOFR, Jacques CÔTÉ a Agnel SFEIR. Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states. \textit{Genes \&{} Development}. Cold Spring Harbor Laboratory Press, 2022, roč.~36, 5-6, s.~313-330. ISSN~0890-9369. Dostupné z: https://dx.doi.org/10.1101/gad.349039.121.

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