Crucial factors governing the electrochemical impedance on
protein-modified surfaces

J 2021

Crucial factors governing the electrochemical impedance on protein-modified surfaces

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Josef HOUSER, Petr SKLÁDAL, Karel LACINA et. al.

Základní údaje

Originální název

Crucial factors governing the electrochemical impedance on protein-modified surfaces

Autoři

SOPOUŠEK, Jakub (203 Česká republika, domácí), Jakub VĚŽNÍK (203 Česká republika, domácí), Josef HOUSER (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Karel LACINA (203 Česká republika, garant, domácí)

Vydání

Electrochimica Acta, OXFORD, Elsevier, 2021, 0013-4686

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.336

Kód RIV

RIV/00216224:14740/21:00119677

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.electacta.2021.138616

UT WoS

000670355000003

Klíčová slova anglicky

Electrochemical impedance spectroscopy; Polystyrene nanoparticles; Polyacrylic acid; Bovine serum albumin; Cationized bovine serum albumin; Ionic strength; Ionic charge

Štítky

CF BIC, CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 10. 2024 17:37, Ing. Marie Švancarová

Anotace

V originále

Three different electrode surfaces - two nanoporous and one modified with polyelectrolyte - provided with positively and negatively charged model proteins were studied at different conditions to determine characteristics of electrochemical impedance spectroscopy in biosensing. The impedance response in the presence of [Fe(CN)(6)(4-/3-) was simultaneously influenced by two effects - ionic attraction/repulsion and steric hindrance of protein components. The ionic interactions dominated at low ionic strength and at low concentrations of redox probe. On the other hand, the steric hindrance of immobilized proteins affecting mass transfer towards the electrode gained impact gradually with the increasing ionic strength and with the concentration of the redox-active component. The simultaneous effect of these two variables can lead to confusing results which are often misinterpreted when evaluating results from impedimetric affinity biosensors. (C) 2021 Elsevier Ltd. All rights reserved.

Návaznosti

EF19_073/0016943, projekt VaV

Název: Interní grantová agentura Masarykovy univerzity

GJ19-16273Y, projekt VaV

Název: ROS-indukované uvolňování léčiv založené na interakcích ferocenylboronátů

Investor: Grantová agentura ČR, ROS-indukované uvolňování léčiv založené na interakcích ferocenylboronátů

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Josef HOUSER, Petr SKLÁDAL a Karel LACINA. Crucial factors governing the electrochemical impedance on protein-modified surfaces. Electrochimica Acta. OXFORD: Elsevier, 2021, roč. 388, AUG, s. 138616-138622. ISSN 0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2021.138616.

@article{1838000,
   author = {Sopoušek, Jakub and Věžník, Jakub and Houser, Josef and Skládal, Petr and Lacina, Karel},
   article_location = {OXFORD},
   article_number = {AUG},
   doi = {http://dx.doi.org/10.1016/j.electacta.2021.138616},
   keywords = {Electrochemical impedance spectroscopy; Polystyrene nanoparticles; Polyacrylic acid; Bovine serum albumin; Cationized bovine serum albumin; Ionic strength; Ionic charge},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Crucial factors governing the electrochemical impedance on protein-modified surfaces},
   url = {https://www.sciencedirect.com/science/article/pii/S0013468621009063?via%3Dihub},
   volume = {388},
   year = {2021}
}

TY  - JOUR
ID  - 1838000
AU  - Sopoušek, Jakub - Věžník, Jakub - Houser, Josef - Skládal, Petr - Lacina, Karel
PY  - 2021
TI  - Crucial factors governing the electrochemical impedance on protein-modified surfaces
JF  - Electrochimica Acta
VL  - 388
IS  - AUG
SP  - 138616
EP  - 138616
PB  - Elsevier
SN  - 00134686
KW  - Electrochemical impedance spectroscopy
KW  - Polystyrene nanoparticles
KW  - Polyacrylic acid
KW  - Bovine serum albumin
KW  - Cationized bovine serum albumin
KW  - Ionic strength
KW  - Ionic charge
UR  - https://www.sciencedirect.com/science/article/pii/S0013468621009063?via%3Dihub
N2  - Three different electrode surfaces - two nanoporous and one modified with polyelectrolyte - provided with positively and negatively charged model proteins were studied at different conditions to determine characteristics of electrochemical impedance spectroscopy in biosensing. The impedance response in the presence of [Fe(CN)(6)(4-/3-) was simultaneously influenced by two effects - ionic attraction/repulsion and steric hindrance of protein components. The ionic interactions dominated at low ionic strength and at low concentrations of redox probe. On the other hand, the steric hindrance of immobilized proteins affecting mass transfer towards the electrode gained impact gradually with the increasing ionic strength and with the concentration of the redox-active component. The simultaneous effect of these two variables can lead to confusing results which are often misinterpreted when evaluating results from impedimetric affinity biosensors. (C) 2021 Elsevier Ltd. All rights reserved.
ER  -

SOPOUŠEK, Jakub, Jakub VĚŽNÍK, Josef HOUSER, Petr SKLÁDAL a Karel LACINA. Crucial factors governing the electrochemical impedance on protein-modified surfaces. \textit{Electrochimica Acta}. OXFORD: Elsevier, 2021, roč.~388, AUG, s.~138616-138622. ISSN~0013-4686. Dostupné z: https://dx.doi.org/10.1016/j.electacta.2021.138616.

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