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@article{1838257,
author = {Golab, Malgorzata and Przybylowska, Martyna and Kubáň, Petr and Itterheimová, Petra and Wozniakiewicz, Michal},
article_number = {19},
doi = {http://dx.doi.org/10.3390/molecules26195749},
keywords = {capillary electrophoresis; tropane alkaloids; atropine; scopolamine; CE-(CD)-D-4; conductivity detection},
language = {eng},
issn = {1420-3049},
journal = {Molecules},
title = {Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids},
url = {https://www.mdpi.com/1420-3049/26/19/5749},
volume = {26},
year = {2021}
}
TY - JOUR
ID - 1838257
AU - Golab, Malgorzata - Przybylowska, Martyna - Kubáň, Petr - Itterheimová, Petra - Wozniakiewicz, Michal
PY - 2021
TI - Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids
JF - Molecules
VL - 26
IS - 19
SP - 5749
EP - 5749
PB - MDPI
SN - 14203049
KW - capillary electrophoresis
KW - tropane alkaloids
KW - atropine
KW - scopolamine
KW - CE-(CD)-D-4
KW - conductivity detection
UR - https://www.mdpi.com/1420-3049/26/19/5749
N2 - A fast method for the determination of tropane alkaloids, using a portable CE instrument with a capacitively coupled contactless conductivity detector (CE-C4D) was developed and validated for determination of atropine and scopolamine in seeds from Solanaceae family plants. Separation was obtained within 5 min, using an optimized background electrolyte consisting of 0.5 M acetic acid with 0.25% (w/v) β-CD. The limit of detection and quantification was 0.5 µg/mL and 1.5 µg/mL, respectively, for both atropine and scopolamine. The developed method was validated with the following parameters—precision (CV): 1.07–2.08%, accuracy of the assay (recovery, RE): 101.0–102.7% and matrix effect (ME): 92.99–94.23%. Moreover, the optimized CE-C4D method was applied to the analysis of plant extracts and pharmaceuticals, proving its applicability and accuracy.
ER -
GOLAB, Malgorzata, Martyna PRZYBYLOWSKA, Petr KUBÁŇ, Petra ITTERHEIMOVÁ a Michal WOZNIAKIEWICZ. Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids. \textit{Molecules}. MDPI, 2021, roč.~26, č.~19, s.~5749-5759. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26195749.
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