Development of CE-(CD)-D-4 Method for Determination Tropane
Alkaloids

J 2021

Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids

GOLAB, Malgorzata, Martyna PRZYBYLOWSKA, Petr KUBÁŇ, Petra ITTERHEIMOVÁ, Michal WOZNIAKIEWICZ et. al.

Základní údaje

Originální název

Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids

Autoři

GOLAB, Malgorzata, Martyna PRZYBYLOWSKA, Petr KUBÁŇ (203 Česká republika), Petra ITTERHEIMOVÁ (203 Česká republika, domácí) a Michal WOZNIAKIEWICZ (garant)

Vydání

Molecules, MDPI, 2021, 1420-3049

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.927

Kód RIV

RIV/00216224:14740/21:00124340

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/molecules26195749

UT WoS

000708212100001

Klíčová slova anglicky

capillary electrophoresis; tropane alkaloids; atropine; scopolamine; CE-(CD)-D-4; conductivity detection

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2022 18:35, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

A fast method for the determination of tropane alkaloids, using a portable CE instrument with a capacitively coupled contactless conductivity detector (CE-C4D) was developed and validated for determination of atropine and scopolamine in seeds from Solanaceae family plants. Separation was obtained within 5 min, using an optimized background electrolyte consisting of 0.5 M acetic acid with 0.25% (w/v) β-CD. The limit of detection and quantification was 0.5 µg/mL and 1.5 µg/mL, respectively, for both atropine and scopolamine. The developed method was validated with the following parameters—precision (CV): 1.07–2.08%, accuracy of the assay (recovery, RE): 101.0–102.7% and matrix effect (ME): 92.99–94.23%. Moreover, the optimized CE-C4D method was applied to the analysis of plant extracts and pharmaceuticals, proving its applicability and accuracy.

Citovat

GOLAB, Malgorzata, Martyna PRZYBYLOWSKA, Petr KUBÁŇ, Petra ITTERHEIMOVÁ a Michal WOZNIAKIEWICZ. Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids. Molecules. MDPI, 2021, roč. 26, č. 19, s. 5749-5759. ISSN 1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26195749.

@article{1838257,
   author = {Golab, Malgorzata and Przybylowska, Martyna and Kubáň, Petr and Itterheimová, Petra and Wozniakiewicz, Michal},
   article_number = {19},
   doi = {http://dx.doi.org/10.3390/molecules26195749},
   keywords = {capillary electrophoresis; tropane alkaloids; atropine; scopolamine; CE-(CD)-D-4; conductivity detection},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids},
   url = {https://www.mdpi.com/1420-3049/26/19/5749},
   volume = {26},
   year = {2021}
}

TY  - JOUR
ID  - 1838257
AU  - Golab, Malgorzata - Przybylowska, Martyna - Kubáň, Petr - Itterheimová, Petra - Wozniakiewicz, Michal
PY  - 2021
TI  - Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids
JF  - Molecules
VL  - 26
IS  - 19
SP  - 5749
EP  - 5749
PB  - MDPI
SN  - 14203049
KW  - capillary electrophoresis
KW  - tropane alkaloids
KW  - atropine
KW  - scopolamine
KW  - CE-(CD)-D-4
KW  - conductivity detection
UR  - https://www.mdpi.com/1420-3049/26/19/5749
N2  - A fast method for the determination of tropane alkaloids, using a portable CE instrument with a capacitively coupled contactless conductivity detector (CE-C4D) was developed and validated for determination of atropine and scopolamine in seeds from Solanaceae family plants. Separation was obtained within 5 min, using an optimized background electrolyte consisting of 0.5 M acetic acid with 0.25% (w/v) β-CD. The limit of detection and quantification was 0.5 µg/mL and 1.5 µg/mL, respectively, for both atropine and scopolamine. The developed method was validated with the following parameters—precision (CV): 1.07–2.08%, accuracy of the assay (recovery, RE): 101.0–102.7% and matrix effect (ME): 92.99–94.23%. Moreover, the optimized CE-C4D method was applied to the analysis of plant extracts and pharmaceuticals, proving its applicability and accuracy.
ER  -

GOLAB, Malgorzata, Martyna PRZYBYLOWSKA, Petr KUBÁŇ, Petra ITTERHEIMOVÁ a Michal WOZNIAKIEWICZ. Development of CE-(CD)-D-4 Method for Determination Tropane Alkaloids. \textit{Molecules}. MDPI, 2021, roč.~26, č.~19, s.~5749-5759. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26195749.

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