Nuclear organization in crucifer genomes: nucleolus-associated
telomere clustering is not a universal interphase configuration
in Brassicaceae

J 2021

Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae

SHAN, Wenbo, Michaela KUBOVÁ, Terezie MALÍK MANDÁKOVÁ a Martin LYSÁK

Základní údaje

Originální název

Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae

Autoři

SHAN, Wenbo (156 Čína, domácí), Michaela KUBOVÁ (203 Česká republika, domácí), Terezie MALÍK MANDÁKOVÁ (203 Česká republika, domácí) a Martin LYSÁK (203 Česká republika, garant, domácí)

Vydání

Plant Journal, Hoboken, Wiley-Blackwell, 2021, 0960-7412

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.091

Kód RIV

RIV/00216224:14740/21:00119689

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/tpj.15459

UT WoS

000694977300001

Klíčová slova anglicky

interphase; chromocenter-loop model; Rabl; centromere; telomere; nucleolus; Arabidopsis thaliana; Crucifereae

Štítky

CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 10. 2024 10:07, Ing. Martina Blahová

Anotace

V originále

Arabidopsis thaliana has become a major plant research model, where interphase nuclear organization exhibits unique features, including nucleolus-associated telomere clustering. The chromocenter (CC)-loop model, or rosette-like configuration, describes intranuclear chromatin organization in Arabidopsis as megabase-long loops anchored in, and emanating from, peripherally positioned CCs, with those containing telomeres associating with the nucleolus. To investigate whether the CC-loop organization is universal across the mustard family (crucifers), the nuclear distributions of centromeres, telomeres and nucleoli were analyzed by fluorescence in situ hybridization in seven diploid species (2n = 10-16) representing major crucifer clades with an up to 26-fold variation in genome size (160-4260 Mb). Nucleolus-associated telomere clustering was confirmed in Arabidopsis (157 Mb) and was newly identified as the major nuclear phenotype in other species with a small genome (215-381 Mb). In large-genome species (2611-4264 Mb), centromeres and telomeres adopted a Rabl-like configuration or dispersed distribution in the nuclear interior; telomeres only rarely associated with the nucleolus. In Arabis cypria (381 Mb) and Bunias orientalis (2611 Mb), tissue-specific patterns deviating from the major nuclear phenotypes were observed in anther and stem tissues, respectively. The rosette-like configuration, including nucleolus-associated telomere clustering in small-genome species from different infrafamiliar clades, suggests that genomic properties rather than phylogenetic position determine the interphase nuclear organization. Our data suggest that nuclear genome size, average chromosome size and degree of longitudinal chromosome compartmentalization affect interphase chromosome organization in crucifer genomes.

Návaznosti

GA18-20134S, projekt VaV

Název: Organizace interfázních chromosomů a časné meiotické párování chromosomů u brukvovitých

Investor: Grantová agentura ČR, Organizace interfázních chromosomů a časné meiotické párování chromosomů u brukvovitých

MUNI/IGA/1352/2020, interní kód MU

Název: Plant meiotic chromosome dynamics in three dimensions

Investor: Masarykova univerzita, Plant meiotic chromosome dynamics in three dimensions

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Citovat

SHAN, Wenbo, Michaela KUBOVÁ, Terezie MALÍK MANDÁKOVÁ a Martin LYSÁK. Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae. Plant Journal. Hoboken: Wiley-Blackwell, 2021, roč. 108, č. 2, s. 528-540. ISSN 0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.15459.

@article{1838542,
   author = {Shan, Wenbo and Kubová, Michaela and Malík Mandáková, Terezie and Lysák, Martin},
   article_location = {Hoboken},
   article_number = {2},
   doi = {http://dx.doi.org/10.1111/tpj.15459},
   keywords = {interphase; chromocenter-loop model; Rabl; centromere; telomere; nucleolus; Arabidopsis thaliana; Crucifereae},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.15459},
   volume = {108},
   year = {2021}
}

TY  - JOUR
ID  - 1838542
AU  - Shan, Wenbo - Kubová, Michaela - Malík Mandáková, Terezie - Lysák, Martin
PY  - 2021
TI  - Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae
JF  - Plant Journal
VL  - 108
IS  - 2
SP  - 528-540
EP  - 528-540
PB  - Wiley-Blackwell
SN  - 09607412
KW  - interphase
KW  - chromocenter-loop model
KW  - Rabl
KW  - centromere
KW  - telomere
KW  - nucleolus
KW  - Arabidopsis thaliana
KW  - Crucifereae
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.15459
N2  - Arabidopsis thaliana has become a major plant research model, where interphase nuclear organization exhibits unique features, including nucleolus-associated telomere clustering. The chromocenter (CC)-loop model, or rosette-like configuration, describes intranuclear chromatin organization in Arabidopsis as megabase-long loops anchored in, and emanating from, peripherally positioned CCs, with those containing telomeres associating with the nucleolus. To investigate whether the CC-loop organization is universal across the mustard family (crucifers), the nuclear distributions of centromeres, telomeres and nucleoli were analyzed by fluorescence in situ hybridization in seven diploid species (2n = 10-16) representing major crucifer clades with an up to 26-fold variation in genome size (160-4260 Mb). Nucleolus-associated telomere clustering was confirmed in Arabidopsis (157 Mb) and was newly identified as the major nuclear phenotype in other species with a small genome (215-381 Mb). In large-genome species (2611-4264 Mb), centromeres and telomeres adopted a Rabl-like configuration or dispersed distribution in the nuclear interior; telomeres only rarely associated with the nucleolus. In Arabis cypria (381 Mb) and Bunias orientalis (2611 Mb), tissue-specific patterns deviating from the major nuclear phenotypes were observed in anther and stem tissues, respectively. The rosette-like configuration, including nucleolus-associated telomere clustering in small-genome species from different infrafamiliar clades, suggests that genomic properties rather than phylogenetic position determine the interphase nuclear organization. Our data suggest that nuclear genome size, average chromosome size and degree of longitudinal chromosome compartmentalization affect interphase chromosome organization in crucifer genomes.
ER  -

SHAN, Wenbo, Michaela KUBOVÁ, Terezie MALÍK MANDÁKOVÁ a Martin LYSÁK. Nuclear organization in crucifer genomes: nucleolus-associated telomere clustering is not a universal interphase configuration in Brassicaceae. \textit{Plant Journal}. Hoboken: Wiley-Blackwell, 2021, roč.~108, č.~2, s.~528-540. ISSN~0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.15459.

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