| KUBÁŇ, Petr, Věra DOSEDĚLOVÁ, Kert MARTMA, Indrek RANNAMA, Karmen REINPOLD a Ruth SHIMMO. Sub-Minute Analysis of Lactate from a Single Blood Drop Using Capillary Electrophoresis with Contactless Conductivity Detection in Monitoring of Athlete Performance. Molecules. MDPI, 2021, roč. 26, č. 19, s. 5817-5825. ISSN 1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26195817. |
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@article{1838559,
author = {Kubáň, Petr and Dosedělová, Věra and Martma, Kert and Rannama, Indrek and Reinpold, Karmen and Shimmo, Ruth},
article_number = {19},
doi = {http://dx.doi.org/10.3390/molecules26195817},
keywords = {capillary electrophoresis; lactate; blood plasma separation; CE-(CD)-D-4; training assessment},
language = {eng},
issn = {1420-3049},
journal = {Molecules},
title = {Sub-Minute Analysis of Lactate from a Single Blood Drop Using Capillary Electrophoresis with Contactless Conductivity Detection in Monitoring of Athlete Performance},
url = {https://www.mdpi.com/1420-3049/26/19/5817},
volume = {26},
year = {2021}
}
TY - JOUR
ID - 1838559
AU - Kubáň, Petr - Dosedělová, Věra - Martma, Kert - Rannama, Indrek - Reinpold, Karmen - Shimmo, Ruth
PY - 2021
TI - Sub-Minute Analysis of Lactate from a Single Blood Drop Using Capillary Electrophoresis with Contactless Conductivity Detection in Monitoring of Athlete Performance
JF - Molecules
VL - 26
IS - 19
SP - 5817
EP - 5817
PB - MDPI
SN - 14203049
KW - capillary electrophoresis
KW - lactate
KW - blood plasma separation
KW - CE-(CD)-D-4
KW - training assessment
UR - https://www.mdpi.com/1420-3049/26/19/5817
N2 - A simple and fast method for the analysis of lactate from a single drop of blood was developed. The finger-prick whole blood sample (10 mu L) was diluted (1:20) with a 7% (w/v) solution of [tris(hydroxymethyl)methylamino] propanesulfonic acid and applied to a blood plasma separation device. The device accommodates a membrane sandwich composed of an asymmetric polysulfone membrane and a supporting textile membrane that allows the collection of blood plasma into a narrow glass capillary in less than 20 s. Separated and simultaneously diluted blood plasma was directly injected into a capillary electrophoresis instrument with a contactless conductivity detector (CE-(CD)-D-4) and analyzed in less than one minute. A separation electrolyte consisted of 10 mmol/L l-histidine, 15 mmol/L dl-glutamic acid, and 30 mu mol/L cetyltrimethylammonium bromide. The whole procedure starting from the finger-prick sampling until the CE-(CD)-D-4 analysis was finished, took less than 5 min and was suitable for monitoring lactate increase in blood plasma during incremental cycling exercise. The observed lactate increase during the experiments measured by the developed CE-(CD)-D-4 method correlated well with the results from a hand-held lactate analyzer (R = 0.9882). The advantage of the developed CE method is the speed, significant savings per analysis, and the possibility to analyze other compounds from blood plasma.
ER -
KUBÁŇ, Petr, Věra DOSEDĚLOVÁ, Kert MARTMA, Indrek RANNAMA, Karmen REINPOLD a Ruth SHIMMO. Sub-Minute Analysis of Lactate from a Single Blood Drop Using Capillary Electrophoresis with Contactless Conductivity Detection in Monitoring of Athlete Performance. \textit{Molecules}. MDPI, 2021, roč.~26, č.~19, s.~5817-5825. ISSN~1420-3049. Dostupné z: https://dx.doi.org/10.3390/molecules26195817.
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