The oligomeric states of elicitins affect the hypersensitive
response and resistance in tobacco

J 2021

The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco

SOLANSKÝ, Martin, Kamil MIKULÁŠEK, Martina ZAPLETALOVÁ, Marek PETŘIVALSKÝ, Annick CHILTZ et. al.

Základní údaje

Originální název

The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco

Autoři

SOLANSKÝ, Martin (203 Česká republika, domácí), Kamil MIKULÁŠEK (203 Česká republika, domácí), Martina ZAPLETALOVÁ (203 Česká republika, domácí), Marek PETŘIVALSKÝ, Annick CHILTZ, Zbyněk ZDRÁHAL (203 Česká republika, domácí), Nathalie LEBORGNE-CASTEL a Jan LOCHMAN (203 Česká republika, garant, domácí)

Vydání

Journal of Experimental Botany, Oxford, Oxford University Press, 2021, 0022-0957

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.298

Kód RIV

RIV/00216224:14310/21:00124352

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1093/jxb/erab011

UT WoS

000642310500030

Klíčová slova anglicky

Cell wall; elicitin beta-CRY; ELICITIN RESPONSE; hypersensitive response; Nicotiana tabacum; oligomeric structure; Phytophthora; resistance; signalling; SUPPRESSOR OF BIR1-1 (SOBIR1); tobacco

Štítky

CF BIC, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 10. 2024 12:47, Ing. Marie Švancarová

Anotace

V originále

Successful plant defence against microbial pathogens is based on early recognition and fast activation of inducible responses. Key mechanisms include detection of microbe-associated molecular patterns by membrane-localized pattern recognition receptors that induce a basal resistance response. A well-described model of such responses to pathogens involves the interactions between Solanaceae plants and proteinaceous elicitors secreted by oomycetes, called elicitins. It has been hypothesized that the formation of oligomeric structures by elicitins could be involved in their recognition and activation of defensive transduction cascades. In this study, we tested this hypothesis using several approaches, and we observed differences in tobacco plant responses induced by the elicitin beta-cryptogein (beta-CRY) and its homodimer, beta-CRYDIM. We also found that the C-terminal domain of elicitins of other ELI (true-elicitin) clades plays a significant role in stabilization of their oligomeric structure and restraint in the cell wall. In addition, covalently cross-linking beta-CRYDIM impaired the formation of signalling complexes, thereby reducing its capacity to elicit the hypersensitive response and resistance in the host plant, with no significant changes in pathogenesis-related protein expression. By revealing the details of the effects of beta-CRY dimerization on recognition and defence responses in tobacco, our results shed light on the poorly understood role of elicitins' oligomeric structures in the interactions between oomycetes and plants.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

SOLANSKÝ, Martin, Kamil MIKULÁŠEK, Martina ZAPLETALOVÁ, Marek PETŘIVALSKÝ, Annick CHILTZ, Zbyněk ZDRÁHAL, Nathalie LEBORGNE-CASTEL a Jan LOCHMAN. The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco. Journal of Experimental Botany. Oxford: Oxford University Press, 2021, roč. 72, č. 8, s. 3219-3234. ISSN 0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/erab011.

@article{1838597,
   author = {Solanský, Martin and Mikulášek, Kamil and Zapletalová, Martina and Petřivalský, Marek and Chiltz, Annick and Zdráhal, Zbyněk and LeborgneandCastel, Nathalie and Lochman, Jan},
   article_location = {Oxford},
   article_number = {8},
   doi = {http://dx.doi.org/10.1093/jxb/erab011},
   keywords = {Cell wall; elicitin beta-CRY; ELICITIN RESPONSE; hypersensitive response; Nicotiana tabacum; oligomeric structure; Phytophthora; resistance; signalling; SUPPRESSOR OF BIR1-1 (SOBIR1); tobacco},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco},
   url = {https://academic.oup.com/jxb/article/72/8/3219/6105174},
   volume = {72},
   year = {2021}
}

TY  - JOUR
ID  - 1838597
AU  - Solanský, Martin - Mikulášek, Kamil - Zapletalová, Martina - Petřivalský, Marek - Chiltz, Annick - Zdráhal, Zbyněk - Leborgne-Castel, Nathalie - Lochman, Jan
PY  - 2021
TI  - The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco
JF  - Journal of Experimental Botany
VL  - 72
IS  - 8
SP  - 3219-3234
EP  - 3219-3234
PB  - Oxford University Press
SN  - 00220957
KW  - Cell wall
KW  - elicitin beta-CRY
KW  - ELICITIN RESPONSE
KW  - hypersensitive response
KW  - Nicotiana tabacum
KW  - oligomeric structure
KW  - Phytophthora
KW  - resistance
KW  - signalling
KW  - SUPPRESSOR OF BIR1-1 (SOBIR1)
KW  - tobacco
UR  - https://academic.oup.com/jxb/article/72/8/3219/6105174
N2  - Successful plant defence against microbial pathogens is based on early recognition and fast activation of inducible responses. Key mechanisms include detection of microbe-associated molecular patterns by membrane-localized pattern recognition receptors that induce a basal resistance response. A well-described model of such responses to pathogens involves the interactions between Solanaceae plants and proteinaceous elicitors secreted by oomycetes, called elicitins. It has been hypothesized that the formation of oligomeric structures by elicitins could be involved in their recognition and activation of defensive transduction cascades. In this study, we tested this hypothesis using several approaches, and we observed differences in tobacco plant responses induced by the elicitin beta-cryptogein (beta-CRY) and its homodimer, beta-CRYDIM. We also found that the C-terminal domain of elicitins of other ELI (true-elicitin) clades plays a significant role in stabilization of their oligomeric structure and restraint in the cell wall. In addition, covalently cross-linking beta-CRYDIM impaired the formation of signalling complexes, thereby reducing its capacity to elicit the hypersensitive response and resistance in the host plant, with no significant changes in pathogenesis-related protein expression. By revealing the details of the effects of beta-CRY dimerization on recognition and defence responses in tobacco, our results shed light on the poorly understood role of elicitins' oligomeric structures in the interactions between oomycetes and plants.
ER  -

SOLANSKÝ, Martin, Kamil MIKULÁŠEK, Martina ZAPLETALOVÁ, Marek PETŘIVALSKÝ, Annick CHILTZ, Zbyněk ZDRÁHAL, Nathalie LEBORGNE-CASTEL a Jan LOCHMAN. The oligomeric states of elicitins affect the hypersensitive response and resistance in tobacco. \textit{Journal of Experimental Botany}. Oxford: Oxford University Press, 2021, roč.~72, č.~8, s.~3219-3234. ISSN~0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/erab011.

Podrobný výpis o publikaci