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@proceedings{1840789,
author = {Deissová, Tereza and Izakovičová Hollá, Lydie and Zákostelská Jirásková, Zuzana and Dolina, Jiří and Kroupa, Radek and Kunovský, Lumír and Pavlovský, Zdeněk and Lipový, Břetislav and Daněk, Zdeněk and Kala, Zdeněk and Slabý, Ondřej and Urban, Ondřej and Navrátil, Vít and Haruštiak, Tomáš and Lischke, Robert and Bořilová Linhartová, Petra},
booktitle = {UEG Week Virtual 2021, United European Gastroenterology Journal 2021; 9 (Supplement 1)},
keywords = {epidermal growth factor; gastroesophageal reflux; polymorphism; case-control study;},
language = {eng},
title = {Elevated epidermal growth factor receptor levels in Barrett’s esophagus},
year = {2021}
}
TY - CONF
ID - 1840789
AU - Deissová, Tereza - Izakovičová Hollá, Lydie - Zákostelská Jirásková, Zuzana - Dolina, Jiří - Kroupa, Radek - Kunovský, Lumír - Pavlovský, Zdeněk - Lipový, Břetislav - Daněk, Zdeněk - Kala, Zdeněk - Slabý, Ondřej - Urban, Ondřej - Navrátil, Vít - Haruštiak, Tomáš - Lischke, Robert - Bořilová Linhartová, Petra
PY - 2021
TI - Elevated epidermal growth factor receptor levels in Barrett’s esophagus
KW - epidermal growth factor
KW - gastroesophageal reflux
KW - polymorphism
KW - case-control study;
N2 - Introduction: Gastroesophageal reflux disease (GERD) can manifest through various symptoms including mucosal damage, in which case we speak of reflux esophagitis (RE), Barrett’s esophagus (BE) or esophageal adenocarcinoma (EAC). The epidermal growth factor (EGF) and its receptor (EGFR) is pivotal in the maintenance of the integrity of the esophageal mucosa and repair of injured tissues. On the other hand, upregulation of the EGF signaling pathway has been shown to lead to neoplastic transformation; in addition, EGFR expression was also associated with GERD progression.1-8 Aims & methods: The aim of our study was to compare the expression of EGF and EGFR in the esophageal tissues with vs. without pathological changes in GERD patients. The EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in 50 fresh esophageal tissue biopsies with/without pathological changes taken from 25 patients with GERD (RE [N=10], BE [N=8], EAC [N=7]). In addition, the EGFR protein expression was examined in 50 corresponding formalin-fixed paraffin esophageal tissues using immunohistochemistry. The mRNA and protein expressions were statistically evaluated using the Wilcoxon signed ranked test in Statistica v13.2. Results: No differences in the EGF or EGFR mRNA expressions in the esophageal tissues with and without pathological changes were detected in neither of the patient groups (p>0.05). However, the EGFR protein levels in BE biopsies were higher than in corresponding samples without metaplastic changes.(p=0.034). Conclusion: Although the study is limited by the small number of patients, our results suggest that EGFR may possibly serve as a biomarker of BE development. This is the first study comparing EGF and its receptor expressions in multiple sites of the esophagus in the same patient with GERD.
ER -
DEISSOVÁ, Tereza, Lydie IZAKOVIČOVÁ HOLLÁ, Zuzana ZÁKOSTELSKÁ JIRÁSKOVÁ, Jiří DOLINA, Radek KROUPA, Lumír KUNOVSKÝ, Zdeněk PAVLOVSKÝ, Břetislav LIPOVÝ, Zdeněk DANĚK, Zdeněk KALA, Ondřej SLABÝ, Ondřej URBAN, Vít NAVRÁTIL, Tomáš HARUŠTIAK, Robert LISCHKE a Petra BOŘILOVÁ LINHARTOVÁ. Elevated epidermal growth factor receptor levels in Barrett’s esophagus. In \textit{UEG Week Virtual 2021, United European Gastroenterology Journal 2021; 9 (Supplement 1)}. 2021.
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