Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane
Fusion and Disruption.

J 2022

Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.

KABELKA, Ivo, Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA, Karl LOHNER et. al.

Základní údaje

Originální název

Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.

Autoři

KABELKA, Ivo (203 Česká republika, domácí), Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA (703 Slovensko, domácí), Karl LOHNER, Georg PABST, Rumiana DIMOVA a Robert VÁCHA (203 Česká republika, garant, domácí)

Vydání

Biophysical Journal, New York, USA, Cell Press, 2022, 0006-3495

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.400

Kód RIV

RIV/00216224:14740/22:00125525

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.bpj.2021.12.035

UT WoS

000765012800016

Klíčová slova anglicky

Cell Membrane; Cryoelectron Microscopy; Lipid Bilayers; Magainins; Membrane Fusion

Štítky

CF CRYO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 10. 2024 11:23, Ing. Jana Kuchtová

Anotace

V originále

We previously speculated that the synergistically enhanced antimicrobial activity of Magainin 2 and PGLa is related to membrane adhesion, fusion, and further membrane remodeling. Here we combined computer simulations with time-resolved in vitro fluorescence microscopy, cryoelectron microscopy, and small-angle X-ray scattering to interrogate such morphological and topological changes of vesicles at nanoscopic and microscopic length scales in real time. Coarse grained simulations revealed formation of an elongated and bent fusion zone between vesicles in the presence of equimolar peptide mixtures. Vesicle adhesion and fusion were observed to occur within a few seconds by cryoelectron microscopy and corroborated by small-angle X-ray scattering measurements. The latter experiments indicated continued and time-extended structural remodeling for individual peptides or chemically linked peptide heterodimers but with different kinetics. Fluorescence microscopy further captured peptide-dependent adhesion, fusion, and occasional bursting of giant unilamellar vesicles a few seconds after peptide addition. The synergistic interactions between the peptides shorten the time response of vesicles and enhance membrane fusogenic and disruption properties of the equimolar mixture compared with the individual peptides.

Návaznosti

GA20-20152S, projekt VaV

Název: Proteinová přitažlivost a selektivita pro buněčné membrány

Investor: Grantová agentura ČR, Protein Affinity and Selectivity to Cellular Membranes

LL2007, projekt VaV

Název: Peptidoví zabijáci bakterií (Akronym: PeptideKillers)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Peptide Killers of Bacteria

LM2015085, projekt VaV

Název: CERIT Scientific Cloud (Akronym: CERIT-SC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CERIT Scientific Cloud

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

KABELKA, Ivo, Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA, Karl LOHNER, Georg PABST, Rumiana DIMOVA a Robert VÁCHA. Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption. Biophysical Journal. New York, USA: Cell Press, 2022, roč. 121, č. 5, s. 852-861. ISSN 0006-3495. Dostupné z: https://dx.doi.org/10.1016/j.bpj.2021.12.035.

@article{1841097,
   author = {Kabelka, Ivo and Georgiev, Vasil and Marx, Lisa and Pajtinka, Peter and Lohner, Karl and Pabst, Georg and Dimova, Rumiana and Vácha, Robert},
   article_location = {New York, USA},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.bpj.2021.12.035},
   keywords = {Cell Membrane; Cryoelectron Microscopy; Lipid Bilayers; Magainins; Membrane Fusion},
   language = {eng},
   issn = {0006-3495},
   journal = {Biophysical Journal},
   title = {Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.},
   url = {https://www.sciencedirect.com/science/article/pii/S000634952103959X?via%3Dihub},
   volume = {121},
   year = {2022}
}

TY  - JOUR
ID  - 1841097
AU  - Kabelka, Ivo - Georgiev, Vasil - Marx, Lisa - Pajtinka, Peter - Lohner, Karl - Pabst, Georg - Dimova, Rumiana - Vácha, Robert
PY  - 2022
TI  - Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption.
JF  - Biophysical Journal
VL  - 121
IS  - 5
SP  - 852-861
EP  - 852-861
PB  - Cell Press
SN  - 00063495
KW  - Cell Membrane
KW  - Cryoelectron Microscopy
KW  - Lipid Bilayers
KW  - Magainins
KW  - Membrane Fusion
UR  - https://www.sciencedirect.com/science/article/pii/S000634952103959X?via%3Dihub
N2  - We previously speculated that the synergistically enhanced antimicrobial activity of Magainin 2 and PGLa is related to membrane adhesion, fusion, and further membrane remodeling. Here we combined computer simulations with time-resolved in vitro fluorescence microscopy, cryoelectron microscopy, and small-angle X-ray scattering to interrogate such morphological and topological changes of vesicles at nanoscopic and microscopic length scales in real time. Coarse grained simulations revealed formation of an elongated and bent fusion zone between vesicles in the presence of equimolar peptide mixtures. Vesicle adhesion and fusion were observed to occur within a few seconds by cryoelectron microscopy and corroborated by small-angle X-ray scattering measurements. The latter experiments indicated continued and time-extended structural remodeling for individual peptides or chemically linked peptide heterodimers but with different kinetics. Fluorescence microscopy further captured peptide-dependent adhesion, fusion, and occasional bursting of giant unilamellar vesicles a few seconds after peptide addition. The synergistic interactions between the peptides shorten the time response of vesicles and enhance membrane fusogenic and disruption properties of the equimolar mixture compared with the individual peptides.
ER  -

KABELKA, Ivo, Vasil GEORGIEV, Lisa MARX, Peter PAJTINKA, Karl LOHNER, Georg PABST, Rumiana DIMOVA a Robert VÁCHA. Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption. \textit{Biophysical Journal}. New York, USA: Cell Press, 2022, roč.~121, č.~5, s.~852-861. ISSN~0006-3495. Dostupné z: https://dx.doi.org/10.1016/j.bpj.2021.12.035.

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