AGERBIRK, Niels, David I. PATTISON, Terezie MALÍK MANDÁKOVÁ, Martin LYSÁK, Sabine MONTAUT and Dan STAERK. Ancient Biosyntheses in an Oil Crop: Glucosinolate Profiles in Limnanthes alba and Its Relatives (Limnanthaceae, Brassicales). Journal of Agricultural and Food Chemistry. American Chemical Society, 2022, vol. 70, No 4, p. 1134-1147. ISSN 0021-8561. Available from: https://dx.doi.org/10.1021/acs.jafc.1c07299.
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Basic information
Original name Ancient Biosyntheses in an Oil Crop: Glucosinolate Profiles in Limnanthes alba and Its Relatives (Limnanthaceae, Brassicales)
Authors AGERBIRK, Niels, David I. PATTISON, Terezie MALÍK MANDÁKOVÁ (203 Czech Republic, belonging to the institution), Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution), Sabine MONTAUT and Dan STAERK.
Edition Journal of Agricultural and Food Chemistry, American Chemical Society, 2022, 0021-8561.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10611 Plant sciences, botany
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 6.100
RIV identification code RIV/00216224:14740/22:00125529
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1021/acs.jafc.1c07299
UT WoS 000748132600001
Keywords in English biosynthesis; evolution; authentic references; spiking; UHPLC; NMR; LC-QToF-MS; QToF MS/MS; mass spectrometry; fragmentation; Limnanthes; Floerkea; Limnanthaceae; Moringaceae; Brassicaceae; crucifers
Tags CF PLANT, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 19/12/2022 10:29.
Abstract
The glucosinolate (GSL) profiles of four Limnanthaceae species, including the oil crop Limnanthes alba (meadowfoam), were investigated by an ultrahigh-performance liquid chromatography-quadrupole time-of -fight tandem mass spectrometry (UHPLC-QToF-MS/MS) analysis of desulfoGSLs after desulfation of native GSLs, supplemented by NMR of desulfated 2-hydroxy-2-methylpropylGSL and 3-methoxybenzylGSL. Leaves, roots, and seeds were investigated, providing an overview of biosynthetic capabilities in the genera Floerkea and Limnanthes. Methoxyl groups on benzylGSLs were in meta but not para positions; two 3,5-disubstituted benzylGSLs are tentatively proposed. 2-Hydroxy-2-methylpropylGSL was accompanied by an isomer that was not a previously reported GSL. The combined GSL profile of the family included GSLs derived from valine, leucine, isoleucine, phenylalanine, and tyrosine, and possibly methionine and tryptophan. Substituted indole GSLs and GSLs derived from chain-elongated amino acids or alanine were searched for but not detected. Hypothetic glycosides of GSLs were detected at low levels. Based on biochemical interpretation, we suggest biosynthetic schemes and gene families (CYP79C, GSOH) relevant for tailoring GSL profiles in Limnanthes crops.
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