Transcriptomic and Proteomic Analysis of Drought Stress
Response in Opium Poppy Plants during the First Week of
Germination

J 2021

Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination

KUNDRATOVA, K., M. BARTAS, P. PECINKA, O. HEJNA, A. RYCHLA et. al.

Základní údaje

Originální název

Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination

Autoři

KUNDRATOVA, K., M. BARTAS, P. PECINKA, O. HEJNA, A. RYCHLA, V. CURN a J. CERVEN

Vydání

PLANTS-BASEL, BASEL, MDPI, 2021, 2223-7747

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.658

Kód RIV

RIV/00216224:14740/21:00124441

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/plants10091878

UT WoS

000701583900001

Klíčová slova anglicky

opium poppy; Papaver somniferum; drought response; transcriptomics; proteomics; dehydrins; gene expression; plant stress

Štítky

CF PROT, ne MU, rivok

Změněno: 23. 3. 2022 12:08, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive element-binding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

KUNDRATOVA, K., M. BARTAS, P. PECINKA, O. HEJNA, A. RYCHLA, V. CURN a J. CERVEN. Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination. PLANTS-BASEL. BASEL: MDPI, 2021, roč. 10, č. 9, s. 1878-1892. ISSN 2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants10091878.

@article{1842611,
   author = {Kundratova, K. and Bartas, M. and Pecinka, P. and Hejna, O. and Rychla, A. and Curn, V. and Cerven, J.},
   article_location = {BASEL},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/plants10091878},
   keywords = {opium poppy; Papaver somniferum; drought response; transcriptomics; proteomics; dehydrins; gene expression; plant stress},
   language = {eng},
   issn = {2223-7747},
   journal = {PLANTS-BASEL},
   title = {Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination},
   url = {https://www.mdpi.com/2223-7747/10/9/1878},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1842611
AU  - Kundratova, K. - Bartas, M. - Pecinka, P. - Hejna, O. - Rychla, A. - Curn, V. - Cerven, J.
PY  - 2021
TI  - Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination
JF  - PLANTS-BASEL
VL  - 10
IS  - 9
SP  - 1878
EP  - 1878
PB  - MDPI
SN  - 22237747
KW  - opium poppy
KW  - Papaver somniferum
KW  - drought response
KW  - transcriptomics
KW  - proteomics
KW  - dehydrins
KW  - gene expression
KW  - plant stress
UR  - https://www.mdpi.com/2223-7747/10/9/1878
N2  - Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive element-binding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages.
ER  -

KUNDRATOVA, K., M. BARTAS, P. PECINKA, O. HEJNA, A. RYCHLA, V. CURN a J. CERVEN. Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination. \textit{PLANTS-BASEL}. BASEL: MDPI, 2021, roč.~10, č.~9, s.~1878-1892. ISSN~2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants10091878.

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