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@article{1842616,
author = {Dlouhy, O. and Javornik, U. and Zsiros, O. and Sket, P. and Karlicky, V. and Spunda, V. and Plavec, J. and Garab, G.},
article_location = {Orlando, Florida},
article_number = {9},
doi = {http://dx.doi.org/10.3390/cells10092354},
keywords = {P-31-NMR; bilayer membrane; DEM-dynamic exchange model; grana; H-II phase; isotropic phase; non-bilayer lipids; non-lamellar lipid phases; thylakoid membranes; structural flexibility},
language = {eng},
issn = {1079-9796},
journal = {Blood Cells, Molecules and Diseases},
title = {Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy},
url = {https://www.mdpi.com/2073-4409/10/9/2354},
volume = {10},
year = {2021}
}
TY - JOUR
ID - 1842616
AU - Dlouhy, O. - Javornik, U. - Zsiros, O. - Sket, P. - Karlicky, V. - Spunda, V. - Plavec, J. - Garab, G.
PY - 2021
TI - Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy
JF - Blood Cells, Molecules and Diseases
VL - 10
IS - 9
SP - 2354
EP - 2354
PB - Academic Press
SN - 10799796
KW - P-31-NMR
KW - bilayer membrane
KW - DEM-dynamic exchange model
KW - grana
KW - H-II phase
KW - isotropic phase
KW - non-bilayer lipids
KW - non-lamellar lipid phases
KW - thylakoid membranes
KW - structural flexibility
UR - https://www.mdpi.com/2073-4409/10/9/2354
N2 - Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been documented that fully functional thylakoid membranes, in addition to the bilayer, contain an inverted hexagonal (H-II) phase and two isotropic phases. To shed light on the origin of these non-lamellar phases, we performed P-31-NMR spectroscopy experiments on sub-chloroplast particles of spinach: stacked, granum and unstacked, stroma thylakoid membranes. These membranes exhibited similar lipid polymorphism as the whole thylakoids. Saturation transfer experiments, applying saturating pulses at characteristic frequencies at 5 degrees C, provided evidence for distinct lipid phases-with component spectra very similar to those derived from mathematical deconvolution of the P-31-NMR spectra. Wheat-germ lipase treatment of samples selectively eliminated the phases exhibiting sharp isotropic peaks, suggesting easier accessibility of these lipids compared to the bilayer and the H-II phases. Gradually increasing lipid exchanges were observed between the bilayer and the two isotropic phases upon gradually elevating the temperature from 5 to 35 degrees C, suggesting close connections between these lipid phases. Data concerning the identity and structural and functional roles of different lipid phases will be presented in the accompanying paper.
ER -
DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY, V. SPUNDA, J. PLAVEC and G. GARAB. Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy. \textit{Blood Cells, Molecules and Diseases}. Orlando, Florida: Academic Press, 2021, vol.~10, No~9, p.~2354-2369. ISSN~1079-9796. Available from: https://dx.doi.org/10.3390/cells10092354.
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